CHPT1 (NM_020244) Human Tagged ORF Clone

CAT#: RC208385

CHPT1 (Myc-DDK-tagged)-Human choline phosphotransferase 1 (CHPT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY412578) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208385 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHPT1
• Synonyms: CPT; CPT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208385 representing NM_020244
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGGCGCCGGGGCCGGGTCCGCGCCGCGCTGGCTGAGGGCGCTGAGCGAGCCGCTGAGCGCGG
  CGCAGCTGCGGCGACTGGAGGAGCACCGCTACAGCGCGGCGGGCGTCTCGCTGCTCGAGCCGCCGCTGCA
  GCTCTACTGGACCTGGCTGCTCCAGTGGATCCCGCTCTGGATGGCCCCCAACTCCATCACCCTGCTGGGG
  CTCGCCGTCAACGTGGTCACCACGCTCGTGCTCATCTCCTACTGTCCCACGGCCACCGAAGAGGCACCAT
  ACTGGACATACCTTTTATGTGCACTGGGACTTTTTATTTACCAGTCACTGGATGCTATTGATGGGAAACA
  AGCCAGAAGAACAAACTCTTGTTCCCCTTTAGGGGAGCTCTTTGACCATGGCTGTGACTCTCTTTCCACA
  GTATTTATGGCAGTGGGAGCTTCAATTGCCGCTCGCTTAGGAACTTATCCTGACTGGTTTTTTTTCTGCT
  CTTTTATTGGGATGTTTGTGTTTTATTGCGCTCATTGGCAGACTTATGTTTCAGGCATGTTGAGATTTGG
  AAAAGTGGATGTAACTGAAATTCAGATAGCTTTAGTGATTGTCTTTGTGTTGTCTGCATTTGGAGGAGCA
  ACAATGTGGGACTATACGATTCCTATTCTAGAAATAAAATTGAAGATCCTTCCAGTTCTTGGATTTCTAG
  GTGGAGTAATATTTTCCTGTTCAAATTATTTCCATGTTATCCTCCATGGTGGTGTTGGCAAGAATGGATC
  CACTATAGCAGGCACCAGTGTCTTGTCACCTGGACTCCACATAGGACTAATTATTATACTGGCAATAATG
  ATCTATAAAAAGTCAGCAACTGATGTGTTTGAAAAGCATCCTTGTCTTTATATCCTAATGTTTGGATGTG
  TCTTTGCTAAAGTCTCACAAAAATTAGTGGTAGCTCACATGACCAAAAGTGAACTATATCTTCAAGACAC
  TGTCTTTTTGGGGCCAGGTCTTTTGTTTTTAGACCAGTACTTTAATAACTTTATAGACGAATATGTTGTT
  CTATGGATGGCAATGGTGATTTCTTCATTTGATATGGTGATATACTTTAGTGCTTTGTGCCTGCAAATTT
  CAAGACACCTTCATCTAAATATATTCAAGACTGCATGTCATCAAGCACCTGAACAGGTTCAAGTTCTTTC
  TTCAAAGAGTCATCAGAATAACATGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208385 representing NM_020244
  Red=Cloning site Green=Tags(s)
  
  MAAGAGAGSAPRWLRALSEPLSAAQLRRLEEHRYSAAGVSLLEPPLQLYWTWLLQWIPLWMAPNSITLLG
  LAVNVVTTLVLISYCPTATEEAPYWTYLLCALGLFIYQSLDAIDGKQARRTNSCSPLGELFDHGCDSLST
  VFMAVGASIAARLGTYPDWFFFCSFIGMFVFYCAHWQTYVSGMLRFGKVDVTEIQIALVIVFVLSAFGGA
  TMWDYTIPILEIKLKILPVLGFLGGVIFSCSNYFHVILHGGVGKNGSTIAGTSVLSPGLHIGLIIILAIM
  IYKKSATDVFEKHPCLYILMFGCVFAKVSQKLVVAHMTKSELYLQDTVFLGPGLLFLDQYFNNFIDEYVV
  LWMAMVISSFDMVIYFSALCLQISRHLHLNIFKTACHQAPEQVQVLSSKSHQNNMD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020244
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020244.1, NM_020244.2, NP_064629.2
• RefSeq Size: 1567
• RefSeq ORF: 1221
• Locus ID: 56994
• Domains: CDP-OH_P_transf
• Protein Families: Transmembrane
• Protein Pathways: Ether lipid metabolism, Glycerophospholipid metabolism, Metabolic pathways
• MW: 44.9 kDa
• Gene Summary: Catalyzes phosphatidylcholine biosynthesis from CDP-choline. It thereby plays a central role in the formation and maintenance of vesicular membranes. [UniProtKB/Swiss-Prot Function]