PLEKHA3 (NM_019091) Human Tagged ORF Clone

CAT#: RC208433

PLEKHA3 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 3 (PLEKHA3)

Product Images

Western blot validation of overexpression lysate (Cat# LY402733) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208433 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PLEKHA3 protein (Cat# TP308433). The protein was produced from HEK293T cells transfected with PLEKHA3 cDNA clone (Cat# RC208433) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHA3
• Synonyms: FAPP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208433 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGGTGTTGTACAAGTGGACCAACTATCTCACAGGCTGGCAGCCTCGTTGGTTTGTTTTAGATA
  ATGGAATCTTATCCTACTATGATTCACAAGATGATGTTTGCAAAGGGAGCAAAGGAAGCATAAAGATGGC
  AGTTTGTGAAATTAAAGTTCATTCAGCAGACAACACAAGAATGGAATTAATCATTCCTGGAGAGCAGCAT
  TTCTACATGAAGGCAGTGAATGCAGCTGAAAGACAGAGGTGGCTGGTCGCTCTGGGGAGCTCCAAAGCAT
  GTTTGACTGATACAAGGACTAAAAAAGAAAAAGAAATAAGTGAAACCAGTGAATCGCTGAAAACCAAAAT
  GTCTGAACTTCGCCTCTACTGTGACCTCTTAATGCAGCAAGTTCATACAATACAGGAATTTGTTCACCAT
  GATGAGAATCATTCATCTCCTAGTGCAGAGAACATGAATGAAGCCTCTTCTCTGCTTAGTGCCACGTGTA
  ACACATTCATCACAACGCTTGAGGAATGTGTGAAGATAGCCAATGCCAAGTTTAAACCTGAGATGTTTCA
  ACTGCACCATCCGGATCCCTTAGTTTCTCCTGTGTCACCTTCTCCTGTTCAAATGATGAAGCGTTCTGTC
  AGCCACCCTGGTTCTTGCAGTTCAGAGAGGAGTAGCCACTCTATAAAAGAACCAGTATCTACACTTCACC
  GACTCTCCCAGCGACGCCGAAGAACCTACTCAGATACAGATTCTTGTAGTGATATTCCTCTTGAAGACCC
  AGATAGACCTGTTCACTGTTCAAAAAATACACTTAATGGAGATTTGGCATCAGCAACCATTCCTGAAGAA
  AGCAGACTTATGGCCAAAAAACAATCTGAATCAGAAGATACTCTTCCATCCTTCTCTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208433 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGVLYKWTNYLTGWQPRWFVLDNGILSYYDSQDDVCKGSKGSIKMAVCEIKVHSADNTRMELIIPGEQH
  FYMKAVNAAERQRWLVALGSSKACLTDTRTKKEKEISETSESLKTKMSELRLYCDLLMQQVHTIQEFVHH
  DENHSSPSAENMNEASSLLSATCNTFITTLEECVKIANAKFKPEMFQLHHPDPLVSPVSPSPVQMMKRSV
  SHPGSCSSERSSHSIKEPVSTLHRLSQRRRRTYSDTDSCSDIPLEDPDRPVHCSKNTLNGDLASATIPEE
  SRLMAKKQSESEDTLPSFSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019091
• ORF Size: 900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019091.2, NM_019091.3, NP_061964.3
• RefSeq Size: 2516 bp
• RefSeq ORF: 903 bp
• Locus ID: 65977
• Domains: PH
• MW: 33.9 kDa
• Gene Summary: Involved in Golgi to cell surface membrane traffic. Induces membrane tubulation. Binds preferentially to phosphatidylinositol 4-phosphate (PtdIns4P). [UniProtKB/Swiss-Prot Function]