SLC15A2 (NM_021082) Human Tagged ORF Clone

CAT#: RC208443

SLC15A2 (Myc-DDK-tagged)-Human solute carrier family 15 (H+/peptide transporter), member 2 (SLC15A2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC15A2
• Synonyms: PEPT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208443 representing NM_021082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCTTTCCAGAAAAATGAGTCCAAGGAAACTCTTTTTTCACCTGTCTCCATTGAAGAGGTACCAC
  CTCGACCACCTAGCCCTCCAAAGAAGCCATCTCCGACAATCTGTGGCTCCAACTATCCACTGAGCATTGC
  CTTCATTGTGGTGAATGAATTCTGCGAGCGCTTTTCCTATTATGGAATGAAAGCTGTGCTGATCCTGTAT
  TTCCTGTATTTCCTGCACTGGAATGAAGATACCTCCACATCTATATACCATGCCTTCAGCAGCCTCTGTT
  ATTTTACTCCCATCCTGGGAGCAGCCATTGCTGACTCGTGGTTGGGAAAATTCAAGACAATCATCTATCT
  CTCCTTGGTGTATGTGCTTGGCCATGTGATCAAGTCCTTGGGTGCCTTACCAATACTGGGAGGACAAGTG
  GTACACACAGTCCTATCATTGATCGGCCTGAGTCTAATAGCTTTGGGGACAGGAGGCATCAAACCCTGTG
  TGGCAGCTTTTGGTGGAGACCAGTTTGAAGAAAAACATGCAGAGGAACGGACTAGATACTTCTCAGTCTT
  CTACCTGTCCATCAATGCAGGGAGCTTGATTTCTACATTTATCACACCCATGCTGAGAGGAGATGTGCAA
  TGTTTTGGAGAAGACTGCTATGCATTGGCTTTTGGAGTTCCAGGACTGCTCATGGTAATTGCACTTGTTG
  TGTTTGCAATGGGAAGCAAAATATACAATAAACCACCCCCTGAAGGAAACATAGTGGCTCAAGTTTTCAA
  ATGTATCTGGTTTGCTATTTCCAATCGTTTCAAGAACCGTTCTGGAGACATTCCAAAGCGACAGCACTGG
  CTAGACTGGGCGGCTGAGAAATATCCAAAGCAGCTCATTATGGATGTAAAGGCACTGACCAGGGTACTAT
  TCCTTTATATCCCATTGCCCATGTTCTGGGCTCTTTTGGATCAGCAGGGTTCACGATGGACTTTGCAAGC
  CATCAGGATGAATAGGAATTTGGGGTTTTTTGTGCTTCAGCCGGACCAGATGCAGGTTCTAAATCCCCTT
  CTGGTTCTTATCTTCATCCCGTTGTTTGACTTTGTCATTTATCGTCTGGTCTCCAAGTGTGGAATTAACT
  TCTCATCACTTAGGAAAATGGCTGTTGGTATGATCCTAGCATGCCTGGCATTTGCAGTTGCGGCAGCTGT
  AGAGATAAAAATAAATGAAATGGCCCCAGCCCAGCCAGGTCCCCAGGAGGTTTTCCTACAAGTCTTGAAT
  CTGGCAGATGATGAGGTGAAGGTGACAGTGGTGGGAAATGAAAACAATTCTCTGTTGATAGAGTCCATCA
  AATCCTTTCAGAAAACACCACACTATTCCAAACTGCACCTGAAAACAAAAAGCCAGGATTTTCACTTCCA
  CCTGAAATATCACAATTTGTCTCTCTACACTGAGCATTCTGTGCAGGAGAAGAACTGGTACAGTCTTGTC
  ATTCGTGAAGATGGGAACAGTATCTCCAGCATGATGGTAAAGGATACAGAAAGCAGAACAACCAATGGGA
  TGACAACCGTGAGGTTTGTTAACACTTTGCATAAAGATGTCAACATCTCCCTGAGTACAGATACCTCTCT
  CAATGTTGGTGAAGACTATGGTGTGTCTGCTTATAGAACTGTGCAAAGAGGAGAATACCCTGCAGTGCAC
  TGTAGAACAGAAGATAAGAACTTTTCTCTGAATTTGGGTCTTCTAGACTTTGGTGCAGCATATCTGTTTG
  TTATTACTAATAACACCAATCAGGGTCTTCAGGCCTGGAAGATTGAAGACATTCCAGCCAACAAAATGTC
  CATTGCGTGGCAGCTACCACAATATGCCCTGGTTACAGCTGGGGAGGTCATGTTCTCTGTCACAGGTCTT
  GAGTTTTCTTATTCTCAGGCTCCCTCTAGCATGAAATCTGTGCTCCAGGCAGCTTGGCTATTGACAATTG
  CAGTTGGGAATATCATCGTGCTTGTTGTGGCACAGTTCAGTGGCCTGGTACAGTGGGCCGAATTCATTTT
  GTTTTCCTGCCTCCTGCTGGTGATCTGCCTGATCTTCTCCATCATGGGCTACTACTATGTTCCTGTAAAG
  ACAGAGGATATGCGGGGTCCAGCAGATAAGCACATTCCTCACATCCAGGGGAACATGATCAAACTAGAGA
  CCAAGAAGACAAAACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208443 representing NM_021082
  Red=Cloning site Green=Tags(s)
  
  MNPFQKNESKETLFSPVSIEEVPPRPPSPPKKPSPTICGSNYPLSIAFIVVNEFCERFSYYGMKAVLILY
  FLYFLHWNEDTSTSIYHAFSSLCYFTPILGAAIADSWLGKFKTIIYLSLVYVLGHVIKSLGALPILGGQV
  VHTVLSLIGLSLIALGTGGIKPCVAAFGGDQFEEKHAEERTRYFSVFYLSINAGSLISTFITPMLRGDVQ
  CFGEDCYALAFGVPGLLMVIALVVFAMGSKIYNKPPPEGNIVAQVFKCIWFAISNRFKNRSGDIPKRQHW
  LDWAAEKYPKQLIMDVKALTRVLFLYIPLPMFWALLDQQGSRWTLQAIRMNRNLGFFVLQPDQMQVLNPL
  LVLIFIPLFDFVIYRLVSKCGINFSSLRKMAVGMILACLAFAVAAAVEIKINEMAPAQPGPQEVFLQVLN
  LADDEVKVTVVGNENNSLLIESIKSFQKTPHYSKLHLKTKSQDFHFHLKYHNLSLYTEHSVQEKNWYSLV
  IREDGNSISSMMVKDTESRTTNGMTTVRFVNTLHKDVNISLSTDTSLNVGEDYGVSAYRTVQRGEYPAVH
  CRTEDKNFSLNLGLLDFGAAYLFVITNNTNQGLQAWKIEDIPANKMSIAWQLPQYALVTAGEVMFSVTGL
  EFSYSQAPSSMKSVLQAAWLLTIAVGNIIVLVVAQFSGLVQWAEFILFSCLLLVICLIFSIMGYYYVPVK
  TEDMRGPADKHIPHIQGNMIKLETKKTKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021082
• ORF Size: 2187 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021082.1, NM_021082.2, NM_021082.3, NP_066568.2
• RefSeq Size: 2846 bp
• RefSeq ORF: 2190 bp
• Locus ID: 6565
• Cytogenetics: 3q13.33
• Domains: PTR2
• Protein Families: Transmembrane
• MW: 81.6 kDa
• Gene Summary: 'The mammalian kidney expresses a proton-coupled peptide transporter that is responsible for the absorption of small peptides, as well as beta-lactam antibiotics and other peptide-like drugs, from the tubular filtrate. This transporter, SLC15A2, belongs to the same gene family as SLC15A1 (MIM 600544), the proton-coupled peptide transporter found in the small intestine (Liu et al, 1995 [PubMed 7756356]).[supplied by OMIM, Feb 2011]'