ERO1LB (ERO1B) (NM_019891) Human Tagged ORF Clone

CAT#: RC208445

ERO1LB (Myc-DDK-tagged)-Human ERO1-like beta (S. cerevisiae) (ERO1LB)

Product Images

Western blot validation of overexpression lysate (Cat# LY402743) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208445 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ERO1B protein (Cat# TP308445). The protein was produced from HEK293T cells transfected with ERO1B cDNA clone (Cat# RC208445) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ERO1B
• Synonyms: Ero1beta; ERO1LB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208445 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAAGGGGTCCGCCGGGCAGGCGCTGGGCAGGGGGTAGCGGCCGCGGTGCAGCTGCTGGTCACCC
  TGAGCTTCCTGCGGAGCGTCGTCGAGGCGCAGGTCACTGGAGTTCTGGATGATTGCTTGTGTGATATTGA
  CAGCATCGATAACTTCAATACCTACAAAATCTTCCCCAAAATAAAAAAATTGCAAGAGAGAGACTATTTT
  CGTTATTACAAGGTTAATCTGAAGCGACCTTGTCCTTTCTGGGCAGAAGATGGCCACTGTTCAATAAAAG
  ACTGTCATGTGGAGCCCTGTCCAGAGAGTAAAATTCCGGTTGGAATAAAAGCTGGGCATTCTAATAAGTA
  CTTGAAAATGGCAAACAATACCAAAGAATTAGAAGTTTGTGAGCAAGCTAATAAACTGGGAGCAATTAAC
  AGCACATTAAGTAATCAAAGCAAAGAAGCTTTCATTGACTGGGCAAGATATGATGATTCACGGGATCACT
  TTTGTGAACTTGATGATGAGAGATCTCCAGCTGCTCAGTATGTAGACCTATTGCTGAACCCAGAGCGTTA
  CACTGGCTATAAAGGGACCTCTGCATGGAGAGTGTGGAACAGCATCTATGAAGAGAACTGTTTCAAGCCT
  CGATCTGTTTATCGTCCTTTAAATCCTCTGGCGCCTAGCCGAGGCGAAGATGATGGAGAATCATTCTACA
  CATGGCTAGAAGGTTTGTGTCTGGAGAAAAGAGTCTTCTATAAGCTTATATCGGGACTTCATGCTAGCAT
  CAATTTACATCTATGCGCAAATTATCTTTTGGAAGAAACCTGGGGTAAGCCCAGTTGGGGACCTAATATT
  AAAGAATTCAAACACCGCTTTGACCCTGTGGAAACCAAGGGAGAAGGTCCAAGAAGGCTCAAGAATCTTT
  ACTTTTTATACTTGATTGAGCTTCGAGCTTTGTCAAAGGTGGCTCCATATTTTGAGCGCTCAATTGTCGA
  TCTTTACACTGGAAATGCAGAAGAAGATGCTGACACAAAAACTCTTCTACTGAATATCTTTCAAGATACA
  AAGTCCTTTCCCATGCACTTTGATGAGAAATCCATGTTTGCAGGTGACAAAAAAGGGGCCAAGTCACTAA
  AGGAGGAATTCCGATTACATTTCAAGAATATCTCCCGTATAATGGACTGTGTTGGATGTGACAAATGCAG
  ATTATGGGGAAAATTACAGACTCAGGGTTTAGGAACTGCCCTGAAGATATTATTCTCTGAAAAAGAAATC
  CAAAAGCTTCCAGAGAATAGTCCATCTAAAGGCTTCCAACTCACCCGACAGGAAATAGTTGCTCTTTTAA
  ATGCTTTTGGAAGGCTTTCTACAAGTATAAGAGACTTACAGAATTTTAAAGTCTTATTACAACACAGTAG
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208445 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQGVRRAGAGQGVAAAVQLLVTLSFLRSVVEAQVTGVLDDCLCDIDSIDNFNTYKIFPKIKKLQERDYF
  RYYKVNLKRPCPFWAEDGHCSIKDCHVEPCPESKIPVGIKAGHSNKYLKMANNTKELEVCEQANKLGAIN
  STLSNQSKEAFIDWARYDDSRDHFCELDDERSPAAQYVDLLLNPERYTGYKGTSAWRVWNSIYEENCFKP
  RSVYRPLNPLAPSRGEDDGESFYTWLEGLCLEKRVFYKLISGLHASINLHLCANYLLEETWGKPSWGPNI
  KEFKHRFDPVETKGEGPRRLKNLYFLYLIELRALSKVAPYFERSIVDLYTGNAEEDADTKTLLLNIFQDT
  KSFPMHFDEKSMFAGDKKGAKSLKEEFRLHFKNISRIMDCVGCDKCRLWGKLQTQGLGTALKILFSEKEI
  QKLPENSPSKGFQLTRQEIVALLNAFGRLSTSIRDLQNFKVLLQHSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019891
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019891.1, NM_019891.2, NM_019891.3, NP_063944.2
• RefSeq Size: 5070 bp
• RefSeq ORF: 1404 bp
• Locus ID: 56605
• Domains: ERO1
• MW: 53.5 kDa