CELF3 (NM_007185) Human Tagged ORF Clone

CAT#: RC208465

CELF3 (Myc-DDK-tagged)-Human CUGBP, Elav-like family member 3 (CELF3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416136) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208465 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CELF3 protein (Cat# TP308465). The protein was produced from HEK293T cells transfected with CELF3 cDNA clone (Cat# RC208465) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CELF3
• Synonyms: BRUNOL1; CAGH4; ERDA4; ETR-1; TNRC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208465 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGCCGGATGCCATCAAGCTGTTTGTGGGGCAGATCCCGAGGCACCTGGAGGAGAAGGACCTGA
  AGCCCATCTTCGAACAGTTTGGTCGGATCTTTGAGCTGACTGTCATCAAGGACAAGTACACCGGGCTGCA
  CAAGGGATGTGCCTTCCTGACATACTGTGCCCGGGATTCAGCCCTGAAGGCCCAGAGCGCCCTGCACGAA
  CAGAAGACGCTTCCAGGGATGAACAGGCCGATCCAGGTCAAGCCAGCCGACAGCGAGAGCCGAGGAGAAG
  ACCGGAAGCTCTTTGTGGGGATGCTAGGGAAGCAGCAGACAGATGAGGACGTCCGGAAGATGTTTGAGCC
  CTTCGGGACCATCGACGAGTGCACTGTGCTCCGGGGGCCAGATGGCACCAGCAAAGGCTGCGCCTTCGTG
  AAGTTCCAGACCCACGCTGAGGCCAAGGCGGCCATCAACACCCTTCACAGCAGCCGGACCCTGCCAGGTG
  CCTCGTCCAGCCTGGTGGTGAAGTTTGCTGACACTGAGAAGGAGCGAGGTCTCCGCCGCATGCAGCAGGT
  GGCCACCCAGTTGGGCATGTTCAGCCCCATCACCCTCCAGTTTGGAGCCTACAGCGCCTACACCCAGGCC
  CTGATGCAGCAGCAGGCGGCCCTGGTAGCGGCTCACAGTGCCTACCTCAGCCCCATGGCCACCATGGCTG
  CCGTGCAGATGCAGCACATGGCTGCCATCAATGCCAATGGCCTCATCGCCACCCCCATCACCCCATCCTC
  AGGAACCAGCACCCCTCCTGCCATCGCTGCCACGCCTGTCTCTGCCATTCCGGCTGCCCTGGGCGTCAAC
  GGCTACAGCCAGGTGCCCACCCAGCCCACTGGGCAGCCTGCCCCTGATGCTCTGTATCCCAACGGGGTTC
  ACCCCTACCCAGCCCAGAGCCCCGCGGCCCCCGTGGACCCCCTGCAGCAGGCCTACGCGGGGATGCAGCA
  CTACACAGCCTACCCAGCAGCCTACAGCCTGGTTGCACCTGCGTTCCCGCAGCCTCCAGCCCTGGTCGCC
  CAGCAGCCCCCACCACCACCTCAACAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAAAGAG
  AAGGCCCTGATGGCTGCAACATCTTCATCTACCACCTGCCCCAGGAGTTCACTGACTCAGAGATCCTCCA
  GATGTTTGTCCCCTTTGGCCACGTCATCTCAGCCAAAGTCTTTGTTGACCGAGCCACCAATCAAAGCAAA
  TGTTTTGGCTTTGTGAGTTTCGACAATCCGGCCAGTGCCCAGGCTGCCATCCAGGCCATGAATGGCTTCC
  AGATCGGCATGAAGCGCCTCAAAGTCCAGCTAAAGCGGCCTAAGGATGCCAACCGGCCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208465 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKEPDAIKLFVGQIPRHLEEKDLKPIFEQFGRIFELTVIKDKYTGLHKGCAFLTYCARDSALKAQSALHE
  QKTLPGMNRPIQVKPADSESRGEDRKLFVGMLGKQQTDEDVRKMFEPFGTIDECTVLRGPDGTSKGCAFV
  KFQTHAEAKAAINTLHSSRTLPGASSSLVVKFADTEKERGLRRMQQVATQLGMFSPITLQFGAYSAYTQA
  LMQQQAALVAAHSAYLSPMATMAAVQMQHMAAINANGLIATPITPSSGTSTPPAIAATPVSAIPAALGVN
  GYSQVPTQPTGQPAPDALYPNGVHPYPAQSPAAPVDPLQQAYAGMQHYTAYPAAYSLVAPAFPQPPALVA
  QQPPPPPQQQQQQQQQQQQQQQREGPDGCNIFIYHLPQEFTDSEILQMFVPFGHVISAKVFVDRATNQSK
  CFGFVSFDNPASAQAAIQAMNGFQIGMKRLKVQLKRPKDANRPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007185
• ORF Size: 1035 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007185.2, NM_007185.3, NM_007185.4, NM_007185.5, NM_007185.6, NP_009116.3
• RefSeq Size: 5615 bp
• RefSeq ORF: 1398 bp
• Locus ID: 11189
• Domains: RRM
• Protein Families: Transcription Factors
• MW: 50.5 kDa
• Gene Summary: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]