Upstream Binding Protein 1 (UBP1) (NM_014517) Human Tagged ORF Clone

CAT#: RC208469

UBP1 (Myc-DDK-tagged)-Human upstream binding protein 1 (LBP-1a) (UBP1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426903) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225928 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBP1
• Synonyms: LBP-1a; LBP-1B; LBP1A; LBP1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208469 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGGTGCTCAAGATGGACGAGGTGATCGAGTCCGGGCTGGTGCACGACTTCGACGCCAGCCTCT
  CGGGCATCGGGCAGGAACTGGGCGCCGGCGCTTACAGCATGAGTGATGTCTTGGCATTGCCCATTTTCAA
  GCAGGAAGATTCCAGCCTTCCATTGGATGGTGAAACAGAGCACCCACCCTTTCAGTATGTGATGTGTGCT
  GCAACGTCACCAGCAGTAAAACTGCATGATGAAACGCTTACTTATTTGAACCAAGGTCAGTCATATGAAA
  TTCGGATGCTGGATAATCGGAAAATGGGTGATATGCCTGAGATCAATGGAAAATTAGTAAAGAGCATCAT
  AAGGGTTGTATTCCATGACAGACGGCTACAATACACAGAGCATCAGCAACTTGAAGGATGGAAGTGGAAT
  CGCCCAGGAGACAGACTTCTTGATTTAGATATTCCAATGTCTGTGGGAATAATTGACACAAGGACGAATC
  CAAGCCAGTTAAATGCGGTTGAATTTCTGTGGGACCCAGCAAAACGCACCTCTGCTTTCATTCAGGTACA
  CTGCATCAGCACAGAATTTACTCCACGGAAGCACGGAGGTGAAAAGGGAGTGCCCTTTAGGATCCAGGTT
  GACGCCTTTAAGCAGAATGAAAATGGAGAATACACAGATCATCTACACTCAGCTAGCTGCCAAATCAAAG
  TTTTTAAGCCTAAAGGTGCAGACAGGAAACAAAAAACTGACCGAGAGAAGATGGAGAAGAGAACAGCTCA
  TGAAAAAGAAAAGTATCAGCCGTCCTATGATACCACAATCCTCACAGAGATGAGGCTTGAGCCTATAATT
  GAAGATGCAGTTGAACATGAGCAGAAAAAGTCCAGCAAGCGGACTTTGCCAGCAGACTACGGTGATTCTC
  TGGCAAAGCGAGGCAGTTGTTCTCCGTGGCCCGATGCCCCCACAGCCTATGTGAATAACAGCCCTTCCCC
  AGCGCCCACTTTCACCTCCCCACAGCAGAGCACTTGCAGTGTCCCAGACAGCAATTCTTCTTCCCCAAAT
  CATCAGGGAGATGGAGCTTCACAGACCTCTGGTGAACAAATTCAGCCTTCAGCTACGATCCAGGAAACAC
  AGCAATGGCTGCTCAAAAACAGATTCTCTTCCTACACAAGACTGTTCTCTAATTTTTCAGGTGCCGACTT
  ATTAAAACTGACAAAGGAGGATTTAGTTCAAATTTGTGGTGCAGCCGATGGAATTCGGCTCTATAATTCA
  CTGAAGTCAAGGTCGGTTAGACCCCGTTTAACCATCTATGTCTGCCGGGAGCAGCCAAGCAGCACAGTGC
  TGCAAGGGCAGCAGCAAGCTGCAAGCAGTGCAAGCGAGAATGGCAGTGGGGCACCCTATGTTTATCATGC
  AATCTACTTGGAAGAAATGATTGCCTCAGAAGTTGCTCGAAAACTTGCGCTGGTGTTTAATATCCCTCTC
  CACCAAATTAATCAGGTTTACAGACAGGGTCCCACCGGTATTCACATTCTTGTTAGTGATCAGATGGTTC
  AGAATTTTCAAGATGAGAGTTGTTTTTTATTCTCCACAGTGAAAGCTGAAAGTAGTGATGGCATCCACAT
  AATTTTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208469 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAWVLKMDEVIESGLVHDFDASLSGIGQELGAGAYSMSDVLALPIFKQEDSSLPLDGETEHPPFQYVMCA
  ATSPAVKLHDETLTYLNQGQSYEIRMLDNRKMGDMPEINGKLVKSIIRVVFHDRRLQYTEHQQLEGWKWN
  RPGDRLLDLDIPMSVGIIDTRTNPSQLNAVEFLWDPAKRTSAFIQVHCISTEFTPRKHGGEKGVPFRIQV
  DAFKQNENGEYTDHLHSASCQIKVFKPKGADRKQKTDREKMEKRTAHEKEKYQPSYDTTILTEMRLEPII
  EDAVEHEQKKSSKRTLPADYGDSLAKRGSCSPWPDAPTAYVNNSPSPAPTFTSPQQSTCSVPDSNSSSPN
  HQGDGASQTSGEQIQPSATIQETQQWLLKNRFSSYTRLFSNFSGADLLKLTKEDLVQICGAADGIRLYNS
  LKSRSVRPRLTIYVCREQPSSTVLQGQQQAASSASENGSGAPYVYHAIYLEEMIASEVARKLALVFNIPL
  HQINQVYRQGPTGIHILVSDQMVQNFQDESCFLFSTVKAESSDGIHIILK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014517
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014517.1, NM_014517.2, NM_014517.3, NM_014517.4, NP_055332.2
• RefSeq Size: 4162 bp
• RefSeq ORF: 1623 bp
• Locus ID: 7342
• Cytogenetics: 3p22.3
• Domains: CP2
• Protein Families: Transcription Factors
• MW: 60.5 kDa
• Gene Summary: ''