DDX11 (NM_152438) Human Tagged ORF Clone

CAT#: RC208483

DDX11 (Myc-DDK-tagged)-Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (DDX11), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDX11
• Synonyms: CHL1; CHLR1; KRG2; WABS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208483 representing NM_152438
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAATGAAACACAGAAGGTTGGTGCCATCCATTTTCCTTTTCCCTTCACACCCTATTCCATCCAGG
  AAGACTTCATGGCAGAGCTGTACCGGGTTTTGGAGGCTGGCAAGATTGGGATATTTGAGAGTCCAACTGG
  CACTGGGAAGTCCTTAAGTCTTATTTGTGGGGCCCTCTCTTGGCTCCGTGACTTTGAACAGAAGAAGCGT
  GAAGAAGAGGCACGACTCCTTGAAACTGGAACTGGCCCCTTACATGATGAGAAAGATGAATCCCTGTGTC
  TGTCTTCTTCCTGCGAAGGGGCTGCAGGCACCCCGAGGCCTGCTGGAGAACCGGCCTGGGTTACTCAGTT
  TGTGCAGAAGAAAGAAGAGAGGGACCTGGTGGACCGACTAAAGGCGGAGCAGGCCAGGAGGAAGCAGCGA
  GAAGAACGCCTGCAGCAGCTGCAGCACAGGGTGCAGCTCAAGTATGCAGCCAAGCGCCTGAGGCAGGAAG
  AAGAAGAAAGAGAGAATCTCCTCCGCCTCAGCAGGGAGATGCTAGAGACAGGCCCGGAGGCTGAGCGGCT
  GGAGCAGCTGGAGTCTGGGGAGGAGGAGCTGGTCCTCGCCGAATACGAGAGTGATGAGGAGAAAAAGGTG
  GCGAGCAGAGTGGATGAGGATGAGGATGACCTGGAGGAAGAACACATAACTAAGATTTATTACTGTAGTC
  GGACACACTCCCAGCTGGCCCAGTTTGTGCATGAGGTGAAGAAGAGCCCCTTTGGCAAGGATGTTCGGCT
  GGTCTCCCTTGGCTCCCGGCAGAACCTTTGTGTAAATGAAGACGTGAAAAGCCTAGGTTCTGTGCAGCTT
  ATCAACGACCGCTGTGTGGACATGCAGAGAAGCAGGCACGAGAAGAAGAAAGGAGCTGAGGAGGAGAAGC
  CAAAGAGGAGGAGGCAGGAGAAGCAGGCAGCCTGCCCCTTCTACAACCACGAGCAGATGGGCCTTCTCCG
  GGATGAGGCCCTGGCAGAGGTGAAGGACATGGAGCAGCTGCTGGCCCTTGGGAAGGAGGCCCGGGCCTGT
  CCCTATTACGGGAGCCGCCTTGCCATCCCTGCAGCCCAGCTGGTGGTGCTGCCCTATCAGATGCTGCTGC
  ATGCGGCCACTCGGCAGGCCGCGGGCATCCGGCTGCAGGACCAGGTGGTGATCATCGACGAGGCGCACAA
  CCTGATCGACACCATCACGGGCATGCACAGCGTGGAGGTCAGCGGCTCCCAGCTCTGCCAGGCCCATTCC
  CAGCTGCTGCAGTACGTGGAGCGATACGGGAAGCGTTTGAAGGCCAAGAACCTGATGTACCTGAAGCAGA
  TCCTGTATTTGCTGGAGAAATTCGTGGCTGTGCTAGGGGGGAACATTAAGCAAAATCCCAATACACAGAG
  TCTGTCACAGACAGGGACGGAGCTGAAGACCATCAACGACTTTCTCTTCCAGAGCCAGATCGACAACATC
  AACCTGTTCAAGGTGCAGCGATACTGTGAGAAGAGCATGATCAGCAGAAAGCTCTTTGGATTCACTGAAC
  GGTACGGAGCAGTGTTCTCATCCCGGGAGCAGCCCAAACTGGCTGGGTTTCAGCAATTCCTGCAGAGCCT
  GCAGCCCAGGACGACTGAAGCTCTTGCAGCCCCTGCAGACGAGAGTCAGGCCAGCACCCTGCGACCAGCT
  TCTCCACTGATGCACATCCAAGGCTTCCTGGCAGCTCTCACTATGGCCAACCAGGACGGCAGGGTCATCC
  TGAGCCGCCAAGGCAGCCTCAGTCAGAGCACCCTGAAGTTTTTGCTCCTGAATCCAGCTGTGCACTTTGC
  CCAAGTGGTGAAGGAATGCCGGGCAGTGGTCATTGCGGGGGGTACCATGCAGCCGGTGTCTGACTTCCGG
  CAGCAGCTGCTGGCCTGTGCCGGGGTGGAAGCTGAGCGCGTGGTGGAGTTTTCCTGTGGTCACGTGATCC
  CTCCAGACAACATCCTGCCCCTCGTCATCTGCAGCGGGATCTCCAACCAGCCGCTGGAATTCACGTTCCA
  GAAAAGAGAGCTGCCTCAGATGATGGACGAGGTGGGTCGCATTCTCTGTAACCTGTGCGGTGTGGTTCCT
  GGAGGGGTGGTCTGTTTCTTCCCCTCCTACGAGTACCTGCGCCAGGTCCATGCCCACTGGGAGAAGGGTG
  GCCTGCTGGGCCGTCTGGCTGCCAGGAAGAAGATATTCCAGGAACCTAAGAGCGCACACCAGGTGGAGCA
  GGTGCTGCTGGCATATTCCAGGTGCATCCAGGCCTGTGGCCAGGAGAGAGGCCAGGTGACAGGGGCCCTG
  CTCCTCTCTGTGGTTGGAGGAAAGATGAGTGAAGGGATCAACTTCTCTGACAACCTAGGCCGGTGTGTGG
  TGATGGTGGGCATGCCCTTCCCCAACATCAGGTCTGCAGAGCTGCAGGAGAAGATGGCCTACTTGGATCA
  AACCCTCAGCCCCCGGCCAGGCACCCCCAGGGAAGGCTCTGGTGGAGAACCTGTGCATGAAGGCCGTCAA
  CCAGTCCATAGGCAGGGCCATCAGGCACCAGAAGGATTTTGCCAGCGTAGTGCTCCTGGACCAGCGATAT
  GCCCGGCCCCCTGTCCTGGCCAAGCTGCCGGCCTGGATCCGAGCCCGTGTGGAGGTCAAAGCTACCTTTG
  GCCCCGCCATTGCTGCTGTGCAGAAGTTTCACCGGGAGAAGTCGGCCTCTTCCTGATGGGCAACCACACC
  ACTGCCTGGCGCCGTGCCCTTCCTTTGTCCTGCCCGCTGGAGACAGTGTTTGTCGTGGGCGTGGTCTGCG
  GGGATCCTGTTACAAAGGTGAAACCCAGGAGGAGAGTGTGGAGTCCAGAGTGCTGCCAGGACCCAGGCAC
  AGGCGTTAGCTCCCGTAGGAGAAAATGGGGGAATCCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208483 representing NM_152438
  Red=Cloning site Green=Tags(s)
  
  MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKR
  EEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQR
  EERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKV
  ASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQL
  INDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGKEARAC
  PYYGSRLAIPAAQLVVLPYQMLLHAATRQAAGIRLQDQVVIIDEAHNLIDTITGMHSVEVSGSQLCQAHS
  QLLQYVERYGKRLKAKNLMYLKQILYLLEKFVAVLGGNIKQNPNTQSLSQTGTELKTINDFLFQSQIDNI
  NLFKVQRYCEKSMISRKLFGFTERYGAVFSSREQPKLAGFQQFLQSLQPRTTEALAAPADESQASTLRPA
  SPLMHIQGFLAALTMANQDGRVILSRQGSLSQSTLKFLLLNPAVHFAQVVKECRAVVIAGGTMQPVSDFR
  QQLLACAGVEAERVVEFSCGHVIPPDNILPLVICSGISNQPLEFTFQKRELPQMMDEVGRILCNLCGVVP
  GGVVCFFPSYEYLRQVHAHWEKGGLLGRLAARKKIFQEPKSAHQVEQVLLAYSRCIQACGQERGQVTGAL
  LLSVVGGKMSEGINFSDNLGRCVVMVGMPFPNIRSAELQEKMAYLDQTLSPRPGTPREGSGGEPVHEGRQ
  PVHRQGHQAPEGFCQRSAPGPAICPAPCPGQAAGLDPSPCGGQSYLWPRHCCCAEVSPGEVGLFLMGNHT
  TAWRRALPLSCPLETVFVVGVVCGDPVTKVKPRRRVWSPECCQDPGTGVSSRRRKWGNPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152438
• ORF Size: 2910 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152438.1, NP_689651.1
• RefSeq Size: 3942 bp
• RefSeq ORF: 2913 bp
• Locus ID: 1663
• Cytogenetics: 12p11.21
• Protein Families: Stem cell - Pluripotency
• MW: 108.1 kDa
• Gene Summary: 'DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]'