Mitocondrial Translational Initiation Factor 3 (MTIF3) (NM_152912) Human Tagged ORF Clone

CAT#: RC208533

MTIF3 (Myc-DDK-tagged)-Human mitochondrial translational initiation factor 3 (MTIF3), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY407210) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208533 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MTIF3 protein (Cat# TP308533). The protein was produced from HEK293T cells transfected with MTIF3 cDNA clone (Cat# RC208533) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTIF3
• Synonyms: IF3mt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208533 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTCTTTTTCTAAAGAGGTTAACACTACAAACTGTAAAGTCTGAAAATAGTTGCATTAGATGTT
  TTGGTAAACACATCCTGCAAAAGACAGCACCAGCACAGTTGTCCCCTATTGCTTCTGCCCCAAGACTCTC
  CTTCCTAATTCATGCAAAAGCCTTTAGTACCGCTGAAGACACCCAGAATGAAGGAAAAAAGATAAAAAAG
  AATAAAACAGCTTTTAGTAACGTTGGAAGAAAAATTAGTCAGCGAGTTATTCACTTATTTGATGAGAAGG
  GCAATGATTTGGGAAACATGCACCGAGCAAATGTGATTAGACTTATGGATGAGCGAGACCTGCGACTGGT
  TCAAAGGAACACCAGCACAGAACCTGCAGAGTATCAGCTCATGACAGGATTGCAGATCCTCCAGGAGCGG
  CAGAGGCTGAGGGAGATGGAGAAGGCGAACCCCAAAACTGGACCAACCCTGAGAAAGGAACTGATTTTGT
  CTTCAAATATTGGACAACATGATTTGGACACAAAGACTAAACAGATTCAGCAGTGGATTAAGAAAAAACA
  CCTAGTCCAGATTACCATAAAGAAAGGAAAAAATGTAGACGTGTCAGAAAATGAAATGGAGGAGATATTT
  CATCAAATACTCCAGACTATGCCTGGAATAGCTACATTCTCATCTAGGCCACAAGCTGTTCAAGGAGGAA
  AAGCTTTAATGTGTGTTCTTCGTGCTTTGAGCAAAAATGAGGAGAAGGCATATAAAGAAACTCAAGAGAC
  CCAGGAAAGAGACACTTTGAACAAAGACCATGGAAATGATAAGGAATCAAATGTTCTGCATCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208533 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALFLKRLTLQTVKSENSCIRCFGKHILQKTAPAQLSPIASAPRLSFLIHAKAFSTAEDTQNEGKKIKK
  NKTAFSNVGRKISQRVIHLFDEKGNDLGNMHRANVIRLMDERDLRLVQRNTSTEPAEYQLMTGLQILQER
  QRLREMEKANPKTGPTLRKELILSSNIGQHDLDTKTKQIQQWIKKKHLVQITIKKGKNVDVSENEMEEIF
  HQILQTMPGIATFSSRPQAVQGGKALMCVLRALSKNEEKAYKETQETQERDTLNKDHGNDKESNVLHQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152912
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152912.1, NM_152912.2, NM_152912.3, NM_152912.4, NP_690876.2
• RefSeq Size: 1037 bp
• RefSeq ORF: 837 bp
• Locus ID: 219402
• Domains: IF3_N
• MW: 31.7 kDa
• Gene Summary: This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq, Oct 2009]