TRP1 (TYRP1) (NM_000550) Human Tagged ORF Clone

CAT#: RC208595

TYRP1 (Myc-DDK-tagged)-Human tyrosinase-related protein 1 (TYRP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY400187) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208595 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TYRP1 protein (Cat# TP308595). The protein was produced from HEK293T cells transfected with TYRP1 cDNA clone (Cat# RC208595) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TYRP1
• Synonyms: b-PROTEIN; CAS2; CATB; GP75; OCA3; TRP; TRP1; TYRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208595 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCTCCTAAACTCCTCTCTCTGGGCTGTATCTTCTTCCCCTTGCTACTTTTTCAGCAGGCCCGGG
  CTCAATTCCCAAGACAGTGTGCCACTGTTGAGGCTTTGAGAAGTGGTATGTGTTGCCCAGACCTGTCCCC
  TGTGTCTGGGCCTGGGACAGACCGCTGTGGCTCATCATCAGGGAGGGGCAGATGTGAGGCAGTGACTGCA
  GACTCCCGGCCCCACAGCCCTCAGTATCCCCATGATGGCAGAGATGATCGGGAGGTCTGGCCCTTGCGCT
  TCTTCAATAGGACATGTCACTGCAACGGCAATTTCTCAGGACACAACTGTGGGACGTGCCGTCCTGGCTG
  GAGAGGAGCTGCCTGTGACCAGAGGGTTCTCATAGTCAGGAGAAATCTTCTGGACTTAAGTAAAGAAGAA
  AAGAACCACTTTGTCCGGGCCCTGGATATGGCAAAGCGCACAACTCACCCTTTATTTGTCATTGCCACCA
  GGAGATCAGAAGAAATACTGGGGCCAGATGGCAACACGCCACAATTTGAGAACATTTCCATTTATAACTA
  CTTTGTTTGGACACACTATTACTCAGTCAAAAAGACTTTCCTTGGGGTAGGACAGGAAAGCTTTGGTGAA
  GTGGATTTCTCTCATGAGGGACCAGCTTTTCTCACATGGCACAGGTACCACCTCCTGCGTCTGGAGAAAG
  ACATGCAGGAAATGTTGCAAGAGCCTTCTTTCTCCCTTCCTTACTGGAATTTTGCAACGGGGAAAAATGT
  CTGTGATATCTGCACGGATGACTTGATGGGATCCAGAAGCAACTTTGATTCCACTCTAATAAGCCCAAAC
  TCTGTCTTTTCTCAATGGCGAGTGGTCTGTGACTCCTTGGAAGATTATGATACCCTGGGAACACTTTGTA
  ACAGCACCGAGGATGGGCCAATTAGGAGAAATCCAGCTGGAAATGTGGCCAGACCAATGGTGCAACGTCT
  TCCTGAACCACAGGATGTCGCTCAGTGCTTGGAAGTTGGTTTATTTGACACGCCTCCTTTTTATTCCAAC
  TCTACAAACAGTTTCCGAAACACAGTGGAAGGTTACAGTGACCCCACGGGAAAGTATGACCCTGCTGTTC
  GAAGTCTTCACAATTTGGCTCATCTATTCCTGAATGGAACAGGGGGACAAACCCATTTGTCTCCAAATGA
  TCCTATTTTTGTCCTCCTGCACACCTTCACAGATGCAGTCTTTGATGAATGGCTGAGGAGATACAATGCT
  GATATATCCACATTTCCATTGGAAAATGCCCCTATTGGACATAATAGACAATACAACATGGTGCCATTCT
  GGCCCCCAGTCACCAACACAGAAATGTTTGTTACTGCTCCAGACAACCTGGGATACACTTATGAAATTCA
  ATGGCCAAGTCGGGAGTTTAGTGTACCTGAGATAATTGCCATAGCAGTAGTTGGCGCTTTGTTACTGGTT
  GCACTCATTTTTGGGACTGCTTCTTATCTGATTCGTGCCAGACGCAGTATGGATGAAGCTAACCAGCCTC
  TCCTCACTGATCAGTATCAATGCTATGCTGAAGAATATGAAAAACTCCAGAATCCTAATCAGTCTGTGGT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208595 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAPKLLSLGCIFFPLLLFQQARAQFPRQCATVEALRSGMCCPDLSPVSGPGTDRCGSSSGRGRCEAVTA
  DSRPHSPQYPHDGRDDREVWPLRFFNRTCHCNGNFSGHNCGTCRPGWRGAACDQRVLIVRRNLLDLSKEE
  KNHFVRALDMAKRTTHPLFVIATRRSEEILGPDGNTPQFENISIYNYFVWTHYYSVKKTFLGVGQESFGE
  VDFSHEGPAFLTWHRYHLLRLEKDMQEMLQEPSFSLPYWNFATGKNVCDICTDDLMGSRSNFDSTLISPN
  SVFSQWRVVCDSLEDYDTLGTLCNSTEDGPIRRNPAGNVARPMVQRLPEPQDVAQCLEVGLFDTPPFYSN
  STNSFRNTVEGYSDPTGKYDPAVRSLHNLAHLFLNGTGGQTHLSPNDPIFVLLHTFTDAVFDEWLRRYNA
  DISTFPLENAPIGHNRQYNMVPFWPPVTNTEMFVTAPDNLGYTYEIQWPSREFSVPEIIAIAVVGALLLV
  ALIFGTASYLIRARRSMDEANQPLLTDQYQCYAEEYEKLQNPNQSVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000550
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000550.1, NM_000550.2, NP_000541.1
• RefSeq Size: 2876 bp
• RefSeq ORF: 1614 bp
• Locus ID: 7306
• Cytogenetics: 9p23
• Domains: tyrosinase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Melanogenesis, Metabolic pathways, Tyrosine metabolism
• MW: 60.7 kDa
• Gene Summary: 'This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009]'