APCDD1 (NM_153000) Human Tagged ORF Clone

CAT#: RC208639

APCDD1 (Myc-DDK-tagged)-Human adenomatosis polyposis coli down-regulated 1 (APCDD1)

Product Images

Western blot validation of overexpression lysate (Cat# LY403498) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208639 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified APCDD1 protein (Cat# TP308639). The protein was produced from HEK293T cells transfected with APCDD1 cDNA clone (Cat# RC208639) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APCDD1
• Synonyms: B7323; DRAPC1; FP7019; HHS; HTS; HYPT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208639 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGGCCGCGCCGCCTCCTGCTCAGATACCTGTTCCCGGCCCTCCTGCTTCACGGGCTGGGAGAGG
  GTTCTGCCCTCCTTCATCCAGACAGCAGGTCTCATCCTAGGTCCTTAGAGAAAAGTGCCTGGAGGGCTTT
  TAAGGAGTCACAGTGCCATCACATGCTCAAACATCTCCACAATGGTGCAAGGATCACAGTGCAGATGCCA
  CCTACAATCGAGGGCCACTGGGTCTCCACAGGCTGTGAAGTAAGGTCAGGCCCAGAGTTCATCACAAGGT
  CCTACAGATTCTACCACAATAACACCTTCAAGGCCTACCAATTTTATTATGGCAGCAACCGGTGCACAAA
  TCCCACTTATACTCTCATCATCCGGGGCAAGATCCGCCTCCGCCAGGCCTCCTGGATCATCCGAGGGGGC
  ACGGAAGCCGACTACCAGCTGCACAACGTCCAGGTGATCTGCCACACAGAGGCGGTGGCCGAGAAGCTCG
  GCCAGCAGGTGAACCGCACATGCCCGGGCTTCCTCGCAGACGGGGGTCCCTGGGTGCAGGACGTGGCCTA
  TGACCTCTGGCGAGAGGAGAACGGCTGTGAGTGCACCAAGGCCGTGAACTTTGCCATGCATGAACTTCAG
  CTCATCCGGGTGGAGAAGCAGTACCTTCACCACAACCTCGACCACCTGGTCGAGGAGCTCTTCCTTGGTG
  ACATTCACACTGATGCCACCCAGAGGATGTTCTACCGGCCCTCCAGTTACCAGCCCCCTCTGCAGAATGC
  CAAGAACCACGACCATGCCTGCATCGCCTGTCGGATCATCTATCGGTCAGACGAGCACCACCCTCCCATC
  CTGCCCCCAAAGGCAGACCTGACCATCGGCCTGCACGGGGAGTGGGTGAGCCAGCGCTGTGAGGTGCGCC
  CCGAAGTCCTCTTCCTCACCCGCCACTTCATCTTCCATGACAACAACAACACCTGGGAGGGCCACTACTA
  CCACTACTCAGACCCGGTGTGCAAGCACCCCACCTTCTCCATCTACGCCCGGGGCCGCTACAGCCGCGGC
  GTCCTCTCGTCCAGGGTCATGGGAGGCACCGAGTTCGTGTTCAAAGTGAATCACATGAAGGTCACCCCCA
  TGGATGCGGCCACAGCCTCACTGCTCAACGTCTTCAACGGGAATGAGTGCGGGGCCGAGGGCTCCTGGCA
  GGTGGGCATCCAGCAGGATGTGACCCACACCAATGGCTGCGTGGCCCTGGGCATCAAACTACCTCACACG
  GAGTACGAGATCTTCAAAATGGAACAGGATGCCCGGGGGCGCTATCTGCTGTTCAACGGTCAGAGGCCCA
  GCGACGGGTCCAGCCCAGACAGGCCAGAGAAGAGAGCCACGTCCTACCAGATGCCCTTGGTCCAGTGTGC
  CTCCTCTTCGCCGAGGGCAGAGGACCTCGCAGAAGACAGTGGAAGCAGCCTGTATGGCCGGGCCCCTGGG
  AGGCACACCTGGTCCCTGCTGCTGGCTGCACTTGCCTGCCTTGTCCCTCTGCTGCATTGGAACATCCGCA
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208639 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSWPRRLLLRYLFPALLLHGLGEGSALLHPDSRSHPRSLEKSAWRAFKESQCHHMLKHLHNGARITVQMP
  PTIEGHWVSTGCEVRSGPEFITRSYRFYHNNTFKAYQFYYGSNRCTNPTYTLIIRGKIRLRQASWIIRGG
  TEADYQLHNVQVICHTEAVAEKLGQQVNRTCPGFLADGGPWVQDVAYDLWREENGCECTKAVNFAMHELQ
  LIRVEKQYLHHNLDHLVEELFLGDIHTDATQRMFYRPSSYQPPLQNAKNHDHACIACRIIYRSDEHHPPI
  LPPKADLTIGLHGEWVSQRCEVRPEVLFLTRHFIFHDNNNTWEGHYYHYSDPVCKHPTFSIYARGRYSRG
  VLSSRVMGGTEFVFKVNHMKVTPMDAATASLLNVFNGNECGAEGSWQVGIQQDVTHTNGCVALGIKLPHT
  EYEIFKMEQDARGRYLLFNGQRPSDGSSPDRPEKRATSYQMPLVQCASSSPRAEDLAEDSGSSLYGRAPG
  RHTWSLLLAALACLVPLLHWNIRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153000
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153000.1, NM_153000.2, NM_153000.3, NM_153000.4, NP_694545.1
• RefSeq Size: 2579 bp
• RefSeq ORF: 1545 bp
• Locus ID: 147495
• Protein Families: Transmembrane
• MW: 58.8 kDa
• Gene Summary: This locus encodes an inhibitor of the Wnt signaling pathway. Mutations at this locus have been associated with hereditary hypotrichosis simplex. Increased expression of this gene may also be associated with colorectal carcinogenesis. [provided by RefSeq, Sep 2010]