HDAC5 (NM_001015053) Human Tagged ORF Clone

CAT#: RC208656

HDAC5 (Myc-DDK-tagged)-Human histone deacetylase 5 (HDAC5), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HDAC5
• Synonyms: HD5; NY-CO-9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208656 representing NM_001015053
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCTCCCAACGAGTCGGCAGATGGGATGTCAGGTCGGGAACCATCCTTGGAAATCCTGCCGCGGA
  CTTCTCTGCACAGCATCCCTGTGACAGTGGAGGTGAAGCCGGTGCTGCCAAGAGCCATGCCCAGTTCCAT
  GGGGGGTGGGGGTGGAGGCAGCCCCAGCCCTGTGGAGCTACGGGGGGCTCTGGTGGGCTCTGTGGACCCC
  ACACTGCGGGAGCAGCAACTGCAGCAGGAGCTCCTGGCGCTCAAGCAGCAGCAGCAGCTGCAGAAGCAGC
  TCCTGTTCGCTGAGTTCCAGAAACAGCATGACCACCTGACAAGGCAGCATGAGGTCCAGCTGCAGAAGCA
  CCTCAAGCAGCAGCAGGAGATGCTGGCAGCCAAGCAGCAGCAGGAGATGCTGGCAGCCAAGCGGCAGCAG
  GAGCTGGAGCAGCAGCGGCAGCGGGAGCAGCAGCGGCAGGAAGAGCTGGAGAAGCAGCGGCTGGAGCAGC
  AGCTGCTCATCCTGCGGAACAAGGAGAAGAGCAAAGAGAGTGCCATTGCCAGCACTGAGGTAAAGCTGAG
  GCTCCAGGAATTCCTCTTGTCGAAGTCAAAGGAGCCCACACCAGGCGGCCTCAACCATTCCCTCCCACAG
  CACCCCAAATGCTGGGGAGCCCACCATGCTTCTTTGGACCAGAGTTCCCCTCCCCAGAGCGGCCCCCCTG
  GGACGCCTCCCTCCTACAAACTGCCTTTGCCTGGGCCCTACGACAGTCGAGACGACTTCCCCCTCCGCAA
  AACAGCCTCTGAACCCAACTTGAAAGTGCGTTCAAGGCTAAAACAGAAGGTGGCTGAGCGGAGAAGCAGT
  CCCCTCCTGCGTCGCAAGGATGGGACTGTTATTAGCACCTTTAAGAAGAGAGCTGTTGAGATCACAGGTG
  CCGGGCCTGGGGCGTCGTCCGTGTGTAACAGCGCACCCGGCTCCGGCCCCAGCTCTCCCAACAGCTCCCA
  CAGCACCATCGCTGAGAATGGCTTTACTGGCTCAGTCCCCAACATCCCCACTGAGATGCTCCCTCAGCAC
  CGAGCCCTCCCTCTGGACAGCTCCCCCAACCAGTTCAGCCTCTACACGTCTCCTTCTCTGCCCAACATCT
  CCCTAGGGCTGCAGGCCACGGTCACTGTCACCAACTCACACCTCACTGCCTCCCCGAAGCTGTCGACACA
  GCAGGAGGCCGAGAGGCAGGCCCTCCAGTCCCTGCGGCAGGGTGGCACGCTGACCGGCAAGTTCATGAGC
  ACATCCTCTATTCCTGGCTGCCTGCTGGGCGTGGCACTGGAGGGCGACGGGAGCCCCCACGGGCATGCCT
  CCCTGCTGCAGCATGTGCTGTTGCTGGAGCAGGCCCGGCAGCAGAGCACCCTCATTGCTGTGCCACTCCA
  CGGGCAGTCCCCACTAGTGACGGGTGAACGTGTGGCCACCAGCATGCGGACGGTAGGCAAGCTCCCGCGG
  CATCGGCCCCTGAGCCGCACTCAGTCCTCACCGCTGCCGCAGAGTCCCCAGGCCCTGCAGCAGCTGGTCA
  TGCAACAACAGCACCAGCAGTTCCTGGAGAAGCAGAAGCAGCAGCAGCTACAGCTGGGCAAGATCCTCAC
  CAAGACAGGGGAGCTGCCCAGGCAGCCCACCACCCACCCTGAGGAGACAGAGGAGGAGCTGACGGAGCAG
  CAGGAGGTCTTGCTGGGGGAGGGAGCCCTGACCATGCCCCGGGAGGGCTCCACAGAGAGTGAGAGCACAC
  AGGAAGACCTGGAGGAGGAGGACGAGGAAGACGATGGGGAGGAGGAGGAGGATTGCATCCAGGTTAAGGA
  CGAGGAGGGCGAGAGTGGTGCTGAGGAGGGGCCCGACTTGGAGGAGCCTGGTGCTGGATACAAAAAACTG
  TTCTCAGATGCCCAGCCGCTGCAGCCTTTGCAGGTGTACCAGGCGCCCCTCAGCCTGGCCACTGTGCCCC
  ACCAGGCCCTGGGCCGTACCCAGTCCTCCCCTGCTGCCCCTGGGGGCATGAAGAGCCCCCCAGACCAGCC
  CGTCAAGCACCTCTTCACCACAGGTGTGGTCTACGACACGTTCATGCTAAAGCACCAGTGCATGTGCGGG
  AACACACACGTGCACCCTGAGCATGCTGGCCGGATCCAGAGCATCTGGTCCCGGCTGCAGGAGACAGGCC
  TGCTTAGCAAGTGCGAGCGGATCCGAGGTCGCAAAGCCACGCTAGATGAGATCCAGACAGTGCACTCTGA
  ATACCACACCCTGCTCTATGGGACCAGTCCCCTCAACCGGCAGAAGCTAGACAGCAAGAAGTTGCTCGGC
  CCCATCAGCCAGAAGATGTATGCTGTGCTGCCTTGTGGGGGCATCGGGGTGGACAGTGACACCGTGTGGA
  ATGAGATGCACTCCTCCAGTGCTGTGCGCATGGCAGTGGGCTGCCTGCTGGAGCTGGCCTTCAAGGTGGC
  TGCAGGAGAGCTCAAGAATGGATTTGCCATCATCCGGCCCCCAGGACACCACGCCGAGGAATCCACAGCC
  ATGGGATTCTGCTTCTTCAACTCTGTAGCCATCACCGCAAAACTCCTACAGCAGAAGTTGAACGTGGGCA
  AGGTCCTCATCGTGGACTGGGACATTCACCATGGCAATGGCACCCAGCAGGCGTTCTACAATGACCCCTC
  TGTGCTCTACATCTCTCTGCATCGCTATGACAACGGGAACTTCTTTCCAGGCTCTGGGGCTCCTGAAGAG
  GTTGGTGGAGGACCAGGCGTGGGGTACAATGTGAACGTGGCATGGACAGGAGGTGTGGACCCCCCCATTG
  GAGACGTGGAGTACCTTACAGCCTTCAGGACAGTGGTGATGCCCATTGCCCACGAGTTCTCACCTGATGT
  GGTCCTAGTCTCCGCCGGGTTTGATGCTGTTGAAGGACATCTGTCTCCTCTGGGTGGCTACTCTGTCACC
  GCCAGATGTTTTGGCCACTTGACCAGGCAGCTGATGACCCTGGCAGGGGGCCGGGTGGTGCTGGCCCTGG
  AGGGAGGCCATGACTTGACCGCCATCTGTGATGCCTCTGAGGCTTGTGTCTCGGCTCTGCTCAGTGTAGA
  GCTGCAGCCCTTGGATGAGGCAGTCTTGCAGCAAAAGCCCAACATCAACGCAGTGGCCACGCTAGAGAAA
  GTCATCGAGATCCAGAGCAAACACTGGAGCTGTGTGCAGAAGTTCGCCGCTGGTCTGGGCCGGTCCCTGC
  GAGAGGCCCAAGCAGGTGAGACCGAGGAGGCCGAGACTGTGAGCGCCATGGCCTTGCTGTCGGTGGGGGC
  CGAGCAGGCCCAGGCTGCGGCAGCCCGGGAACACAGCCCCAGGCCGGCAGAGGAGCCCATGGAGCAGGAG
  CCTGCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208656 representing NM_001015053
  Red=Cloning site Green=Tags(s)
  
  MNSPNESADGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDP
  TLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQ
  ELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQ
  HPKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSS
  PLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQH
  RALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMS
  TSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPR
  HRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQ
  QEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKL
  FSDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCG
  NTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLG
  PISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTA
  MGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEE
  VGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVT
  ARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEK
  VIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQE
  PAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001015053
• ORF Size: 3369 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001015053.1, NP_001015053.1
• RefSeq Size: 5327
• RefSeq ORF: 3372
• Locus ID: 10014
• Protein Families: Druggable Genome, Transcription Factors
• MW: 122 kDa
• Gene Summary: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]