Carboxypeptidase H (CPE) (NM_001873) Human Tagged ORF Clone
CAT#: RC208791
- TrueORF®
CPE (Myc-DDK-tagged)-Human carboxypeptidase E (CPE)
"NM_001873" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CPE |
Synonyms | CPH |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208791 representing NM_001873
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGCGAGGGGGCAGCGCGCTGCTGGCTCTGTGCGGGGCACTGGCTGCCTGCGGGTGGCTCCTGG GCGCCGAAGCCCAGGAGCCCGGGGCGCCCGCGGCGGGCATGAGGCGGCGCCGGCGGCTGCAGCAAGAGGA CGGCATCTCCTTCGAGTACCACCGCTACCCCGAGCTGCGCGAGGCGCTCGTGTCCGTGTGGCTGCAGTGC ACCGCCATCAGCAGGATTTACACGGTGGGGCGCAGCTTCGAGGGCCGGGAGCTCCTGGTCATCGAGCTGT CCGACAACCCTGGCGTCCATGAGCCTGGTGAGCCTGAATTTAAATACATTGGGAATATGCATGGGAATGA GGCTGTTGGACGAGAACTGCTCATTTTCTTGGCCCAGTACCTATGCAACGAATACCAGAAGGGGAACGAG ACAATTGTCAACCTGATCCACAGTACCCGCATTCACATCATGCCTTCCCTGAACCCAGATGGCTTTGAGA AGGCAGCGTCTCAGCCTGGTGAACTCAAGGACTGGTTTGTGGGTCGAAGCAATGCCCAGGGAATAGATCT GAACCGGAACTTTCCAGACCTGGATAGGATAGTGTACGTGAATGAGAAAGAAGGTGGTCCAAATAATCAT CTGTTGAAAAATATGAAGAAAATTGTGGATCAAAACACAAAGCTTGCTCCTGAGACCAAGGCTGTCATTC ATTGGATTATGGATATTCCTTTTGTGCTTTCTGCCAATCTCCATGGAGGAGACCTTGTGGCCAATTATCC ATATGATGAGACGCGGAGTGGTAGTGCTCACGAATACAGCTCCTCCCCAGATGACGCCATTTTCCAAAGC TTGGCCCGGGCATACTCTTCTTTCAACCCGGCCATGTCTGACCCCAATCGGCCACCATGTCGCAAGAATG ATGATGACAGCAGCTTTGTAGATGGAACCACCAACGGTGGTGCTTGGTACAGCGTACCTGGAGGGATGCA AGACTTCAATTACCTTAGCAGCAACTGTTTTGAGATCACCGTGGAGCTTAGCTGTGAGAAGTTCCCACCT GAAGAGACTCTGAAGACCTACTGGGAGGATAACAAAAACTCCCTCATTAGCTACCTTGAGCAGATACACC GAGGAGTTAAAGGATTTGTCCGAGACCTTCAAGGTAACCCAATTGCGAATGCCACCATCTCCGTGGAAGG AATAGACCACGATGTTACATCCGCAAAGGATGGTGATTACTGGAGATTGCTTATACCTGGAAACTATAAA CTTACAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGGCAGTTCCTTACAGCCCTGCTGCTG GGGTTGATTTTGAACTGGAGTCATTTTCTGAAAGGAAAGAAGAGGAGAAGGAAGAATTGATGGAATGGTG GAAAATGATGTCAGAAACTTTAAATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208791 representing NM_001873
Red=Cloning site Green=Tags(s) MAGRGGSALLALCGALAACGWLLGAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQC TAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNE TIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNH LLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQS LARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPP EETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYK LTASAPGYLAITKKVAVPYSPAAGVDFELESFSERKEEEKEELMEWWKMMSETLNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001873 |
ORF Size | 1428 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001873.1, NM_001873.2, NM_001873.3, NP_001864.1 |
RefSeq Size | 2443 |
RefSeq ORF | 1431 |
Locus ID | 1363 |
Domains | Zn_carbOpept |
Protein Families | Druggable Genome, Protease, Secreted Protein |
Protein Pathways | Type I diabetes mellitus |
MW | 53.15 kDa |
Gene Summary | This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC118995 | CPE (untagged)-Human carboxypeptidase E (CPE) |
USD 760.00 |
|
RG208791 | CPE (GFP-tagged) - Human carboxypeptidase E (CPE) |
USD 460.00 |
|
RC208791L1 | Lenti-ORF clone of CPE (Myc-DDK-tagged)-Human carboxypeptidase E (CPE) |
USD 768.00 |
|
RC208791L2 | Lenti-ORF clone of CPE (mGFP-tagged)-Human carboxypeptidase E (CPE) |
USD 620.00 |
|
RC208791L3 | Lenti-ORF clone of CPE (Myc-DDK-tagged)-Human carboxypeptidase E (CPE) |
USD 768.00 |
|
RC208791L4 | Lenti-ORF clone of CPE (mGFP-tagged)-Human carboxypeptidase E (CPE) |
USD 620.00 |
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