SRGAP1 (NM_020762) Human Tagged ORF Clone

CAT#: RC208812

SRGAP1 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 1 (SRGAP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY412319) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208812 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SRGAP1 protein (Cat# TP308812). The protein was produced from HEK293T cells transfected with SRGAP1 cDNA clone (Cat# RC208812) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRGAP1
• Synonyms: ARHGAP13; NMTC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208812 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAA
  TTCGAGCTCAACTGGTAGAACAACAAAAATGCCTGGAGCAGCAAACGGAGATGCGAGTTCAGCTTCTCCA
  GGATCTGCAAGATTTCTTCCGAAAAAAAGCTGAAATTGAGACGGAATATTCCCGGAATCTAGAGAAGTTA
  GCAGAAAGGTTCATGGCAAAAACAAGAAGCACTAAGGATCATCAACAATACAAGAAAGACCAGAACCTGT
  TGTCTCCAGTGAACTGCTGGTATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGCAACCTT
  GAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGATAAGTGAGGATTCTACCAGGATGTTT
  AAAAAGAGCAAAGAGATTGCATTCCAACTTCATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGG
  TGATGAAAACATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGCTGAAAGAGGCCGAAAA
  ACAAGAGGAAAAGCAAATTGGGAGATCTGGTGATCCAGTCTTCCATATTCGACTAGAGGAGAGACATCAA
  CGGCGAAGCTCTGTAAAGAAAATTGAAAAAATGAAAGAAAAAAGACAAGCAAAATATTCAGAAAATAAGC
  TAAAATCAATTAAGGCACGGAACGAATATCTCCTAACACTTGAAGCCACCAATGCCTCAGTTTTCAAGTA
  CTATATTCATGATCTTTCTGATTTAATTGATTGCTGTGATCTTGGCTACCATGCAAGTCTGAACAGAGCC
  CTAAGAACATATCTGTCTGCGGAGTACAACCTTGAAACCTCCAGACATGAGGGCTTAGACATTATTGAGA
  ATGCAGTTGATAATTTAGAGCCCAGGAGCGATAAGCAGAGATTCATGGAGATGTACCCTGCTGCGTTCTG
  TCCACCAATGAAGTTTGAGTTTCAGTCTCACATGGGTGATGAGGTGTGCCAGGTCAGTGCCCAGCAGCCA
  GTCCAGGCAGAGCTCATGCTCAGGTACCAACAGTTGCAGTCCCGCCTTGCCACGCTCAAAATCGAGAATG
  AAGAGGTTAAGAAAACGACTGAAGCCACCTTGCAGACGATACAAGATATGGTCACCATCGAGGACTATGA
  TGTTTCTGAATGCTTCCAGCACAGTCGTTCCACAGAATCTGTGAAGTCCACTGTCTCTGAAACCTACCTG
  AGTAAACCCAGCATCGCCAAGAGAAGAGCCAACCAGCAGGAAACTGAACAGTTCTACTTCATGAAACTCA
  GAGAATATTTGGAAGGCAGTAATCTCATCACAAAACTTCAAGCCAAACATGACTTGCTGCAGAGGACCCT
  GGGAGAAGGTCATAGAGCTGAATATATGACTACAAGGCCTCCAAATGTTCCCCCTAAGCCCCAGAAACAC
  AGGAAGTCCAGGCCCCGCTCACAGTATAATACTAAGTTGTTTAATGGGGATTTGGAAACATTCGTCAAGG
  ACTCAGGACAGGTTATTCCCCTCATTGTGGAAAGCTGTATTCGGTTCATCAATCTCTATGGTCTTCAGCA
  TCAGGGGATTTTCAGAGTGTCTGGTTCCCAGGTGGAAGTCAATGATATTAAAAATTCATTTGAGAGAGGT
  GAAAATCCTTTGGCTGATGACCAGAGTAACCATGATATTAACTCAGTTGCTGGCGTTCTGAAGCTCTATT
  TCCGTGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGATTTAACGATCTGATTTCTTGTATCAGAATAGA
  TAATCTCTATGAGAGGGCGCTTCACATCCGCAAACTCCTCCTGACTTTGCCCAGGTCGGTCCTTATAGTG
  ATGAGGTACCTCTTTGCCTTCCTCAATCATCTATCACAGTACAGCGATGAGAATATGATGGACCCTTATA
  ACCTGGCCATTTGCTTTGGCCCAACATTGATGCCTGTCCCAGAAATACAGGATCAAGTGTCTTGCCAGGC
  ACATGTGAATGAAATTATCAAAACCATCATCATCCACCATGAGACTATTTTCCCAGATGCTAAAGAGCTG
  GATGGCCCTGTTTATGAGAAATGTATGGCTGGAGATGACTATTGCGACAGCCCATACAGTGAGCACGGTA
  CATTGGAGGAAGTGGACCAAGATGCTGGTACAGAGCCCCACACAAGTGAAGATGAATGTGAGCCAATAGA
  AGCAATAGCCAAGTTTGACTATGTTGGGCGGTCCGCCAGAGAACTATCCTTCAAGAAGGGTGCCTCCCTG
  CTGCTGTATCACCGTGCATCTGAGGACTGGTGGGAAGGCAGGCACAACGGGATTGACGGGCTGGTGCCTC
  ACCAGTATATAGTGGTGCAGGATATGGATGATACGTTTTCAGACACTCTGAGCCAAAAAGCCGACAGTGA
  GGCCAGCAGTGGGCCAGTCACGGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACAGACCGTCAT
  CCTGACGGCTATTTAGCCAGGCAACGAAAAAGAGGAGAGCCACCCCCTCCAGTAAGGCGTCCTGGCAGGA
  CCAGTGATGGCCATTGCCCGCTCCACCCTCCACATGCCCTTTCTAACTCCTCAGTTGACCTAGGGTCCCC
  AAGCCTTGCCAGTCACCCCCGGGGCCTGCTGCAGAACCGTGGCCTCAACAATGACAGTCCTGAGCGGAGG
  CGCAGGCCTGGCCATGGCAGCCTGACCAACATCAGCCGGCACGACTCCCTCAAGAAGATCGACAGCCCTC
  CCATTAGAAGGTCCACGTCATCAGGGCAATACACGGGCTTCAATGACCACAAGCCACTGGACCCAGAGAC
  AATTGCTCAGGATATTGAAGAAACGATGAACACAGCTTTGAATGAACTCCGAGAACTGGAGAGACAGAGC
  ACAGCAAAGCATGCCCCTGATGTGGTGCTGGATACCCTGGAGCAAGTGAAAAACTCTCCCACCCCTGCCA
  CTTCCACGGAATCTCTCAGCCCTTTGCACAACGTTGCCCTCAGGAGCTCCGAGCCTCAGATTCGACGTAG
  CACGAGCTCCTCCAGTGACACAATGAGTACTTTCAAGCCTATGGTGGCACCCAGAATGGGCGTGCAGCTG
  AAGCCTCCAGCCCTTAGGCCAAAACCTGCTGTTCTTCCAAAAACAAATCCTACCATAGGACCTGCCCCAC
  CTCCCCAGGGTCCAACAGACAAGTCATGCACAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208812 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKL
  AERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMF
  KKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQ
  RRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRA
  LRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQP
  VQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYL
  SKPSIAKRRANQQETEQFYFMKLREYLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTRPPNVPPKPQKH
  RKSRPRSQYNTKLFNGDLETFVKDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERG
  ENPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYERALHIRKLLLTLPRSVLIV
  MRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAKEL
  DGPVYEKCMAGDDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASL
  LLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRH
  PDGYLARQRKRGEPPPPVRRPGRTSDGHCPLHPPHALSNSSVDLGSPSLASHPRGLLQNRGLNNDSPERR
  RRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQS
  TAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQL
  KPPALRPKPAVLPKTNPTIGPAPPPQGPTDKSCTM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020762
• ORF Size: 3255 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020762.1, NM_020762.2, NM_020762.3, NP_065813.1
• RefSeq Size: 8475 bp
• RefSeq ORF: 3258 bp
• Locus ID: 57522
• Protein Pathways: Axon guidance
• MW: 124.3 kDa
• Gene Summary: The protein encoded by this gene is a GTPase activator, working with the GTPase CDC42 to negatively regulate neuronal migration. The encoded protein interacts with the transmembrane receptor ROBO1 to inactivate CDC42. [provided by RefSeq, Sep 2016]