RIPK5 (DSTYK) (NM_199462) Human Tagged ORF Clone

CAT#: RC208819

DSTYK (Myc-DDK-tagged)-Human dual serine/threonine and tyrosine protein kinase (DSTYK), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY404577) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208819 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DSTYK
• Synonyms: CAKUT1; DustyPK; HDCMD38P; RIP5; RIPK5; SPG23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208819 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCGACGGGGTGCCATGGGGCAGCGAGCCCGTCTCGGGTCCCGGCCCCGGCGGCGGCGGAATGA
  TCCGCGAGCTGTGCCGGGGCTTCGGCCGCTACCGCCGCTACCTGGGACGGCTGCGACAGAACCTGCGCGA
  GACCCAGAAGTTCTTCCGCGACATCAAGTGCTCCCACAACCACACTTGTCTCTCCTCCCTCACGGGCGGC
  GGCGGGGCCGAGCGCGGCCCTGCAGGCGATGTCGCCGAAACCGGGCTGCAGGCGGGCCAACTGAGCTGCA
  TTTCCTTCCCACCTAAGGAAGAGAAGTACCTCCAGCAGATTGTGGACTGCCTCCCTTGCATACTGATCCT
  CGGCCAGGATTGTAACGTCAAGTGCCAGCTGTTGAATCTGCTGTTGGGGGTGCAGGTGCTTCCCACCACC
  AAGCTGGGCAGTGAGGAGAGCTGTAAGCTTCGGCGCCTCCGCTTCACCTATGGGACTCAGACTCGGGTCA
  GCCTGGCGCTCCCTGGACAGTATGAACTAGTGCACACGCTGGTTGCTCATCAGGGCAACTGGGAGACCAT
  CCCTGAGGAGGATCTGGAGGTCCAAGAGAACAATGAGGATGCTGCTCATGTTTTAGCGGAACTGGAGGTA
  ACGATGCACCATGCTCTCTTACAGGAAGTGGACGTTGTGGTAGCACCATGCCAAGGCCTCCGGCCCACAG
  TGGATGTTCTGGGTGACTTGGTGAATGATTTCTTGCCTGTGATAACCTATGCACTCCACAAAGATGAACT
  CTCTGAGAGGGATGAGCAAGAGCTTCAGGAAATCCGAAAGTATTTCTCCTTTCCTGTATTCTTTTTCAAA
  GTGCCGAAACTGGGCTCGGAGATAATAGACTCCTCAACCAGGAGAATGGAGAGCGAAAGATCACCGCTTT
  ATCGCCAGCTAATTGACCTGGGCTATCTGAGCAGCAGTCACTGGAACTGTGGGGCTCCTGGCCAGGATAC
  TAAAGCTCAGAGCATGTTGGTGGAACAGAGTGAAAAGCTGAGACACTTGAGCACATTTTCTCACCAGGTG
  TTACAGACTCGCCTGGTGGATGCAGCCAAGGCCCTGAACCTGGTGCACTGCCACTGCCTTGACATCTTTA
  TTAACCAGGCATTTGACATGCAGCGGGACCTGCAGATCACTCCCAAACGTCTGGAATATACTCGAAAAAA
  GGAGAATGAGTTGTATGAATCATTGATGAATATTGCCAACCGAAAGCAGGAGGAAATGAAGGATATGATT
  GTTGAGACACTTAATACCATGAAGGAGGAACTTCTGGATGATGCTACTAACATGGAGTTTAAAGACGTCA
  TTGTCCCTGAGAATGGAGAACCAGTAGGCACCAGAGAGATCAAATGCTGCATCCGACAGATCCAGGAACT
  CATCATCTCCCGACTTAATCAGGCAGTGGCTAATAAGCTGATCAGCTCAGTGGATTACCTGAGGGAAAGC
  TTCGTCGGAACCCTGGAACGATGTCTGCAGAGCCTGGAGAAGTCTCAGGATGTCTCAGTTCACATCACCA
  GTAATTATCTCAAACAGATCTTAAATGCTGCCTATCATGTTGAAGTCACGTTTCACTCAGGGTCGTCAGT
  TACAAGGATGCTATGGGAGCAAATCAAACAGATCATCCAGCGCATCACATGGGTGAGCCCACCTGCCATC
  ACTCTGGAATGGAAGAGGAAGGTGGCCCAGGAAGCCATTGAGAGCCTCAGCGCCTCCAAATTGGCTAAGA
  GCATTTGCAGCCAATTCCGGACTCGGCTCAATAGTTCCCACGAGGCTTTTGCAGCCTCCTTGCGGCAGCT
  GGAAGCTGGCCACTCAGGCCGGTTAGAGAAAACGGAAGATCTATGGCTGAGGGTTCGGAAAGATCATGCT
  CCCCGCCTGGCCCGCCTTTCTCTGGAAAGCCGTTCTTTACAGGATGTCTTGCTTCATCGTAAACCTAAAC
  TGGGACAGGAACTGGGCCGGGGCCAGTATGGTGTGGTATACCTGTGTGACAACTGGGGAGGACACTTCCC
  TTGTGCCCTCAAATCAGTTGTCCCTCCAGATGAGAAGCACTGGAATGATCTGGCTTTGGAATTTCACTAT
  ATGAGGTCTCTGCCGAAGCATGAGCGATTGGTGGATCTCCATGGTTCAGTCATTGACTACAACTATGGTG
  GTGGCTCCAGCATTGCTGTGCTCCTCATTATGGAGCGGCTACACCGGGATCTCTACACAGGGCTGAAGGC
  TGGGCTGACCCTGGAGACACGTTTGCAGATAGCACTAGATGTGGTGGAGGGAATCCGCTTCCTGCACAGC
  CAGGGACTTGTCCATCGTGATATCAAACTGAAAAATGTGCTGCTGGATAAGCAGAACCGTGCCAAGATCA
  CTGACTTAGGATTCTGCAAGCCAGAGGCCATGATGTCAGGCAGCATTGTGGGGACACCAATCCATATGGC
  CCCTGAACTTTTCACAGGGGCTCGCCCAGAACGTCTTCCTGTGTTTGATGAGGAGTGCTGGCAGTTGATG
  GAAGCCTGTTGGGATGGCGACCCCTTGAAGAGGCCTCTCTTGGGCATTGTCCAGCCCATGCTCCAGGGCA
  TCATGAATCGGCTCTGCAAGTCCAATTCTGAGCAGCCAAACAGAGGACTAGATGATTCTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208819 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGDGVPWGSEPVSGPGPGGGGMIRELCRGFGRYRRYLGRLRQNLRETQKFFRDIKCSHNHTCLSSLTGG
  GGAERGPAGDVAETGLQAGQLSCISFPPKEEKYLQQIVDCLPCILILGQDCNVKCQLLNLLLGVQVLPTT
  KLGSEESCKLRRLRFTYGTQTRVSLALPGQYELVHTLVAHQGNWETIPEEDLEVQENNEDAAHVLAELEV
  TMHHALLQEVDVVVAPCQGLRPTVDVLGDLVNDFLPVITYALHKDELSERDEQELQEIRKYFSFPVFFFK
  VPKLGSEIIDSSTRRMESERSPLYRQLIDLGYLSSSHWNCGAPGQDTKAQSMLVEQSEKLRHLSTFSHQV
  LQTRLVDAAKALNLVHCHCLDIFINQAFDMQRDLQITPKRLEYTRKKENELYESLMNIANRKQEEMKDMI
  VETLNTMKEELLDDATNMEFKDVIVPENGEPVGTREIKCCIRQIQELIISRLNQAVANKLISSVDYLRES
  FVGTLERCLQSLEKSQDVSVHITSNYLKQILNAAYHVEVTFHSGSSVTRMLWEQIKQIIQRITWVSPPAI
  TLEWKRKVAQEAIESLSASKLAKSICSQFRTRLNSSHEAFAASLRQLEAGHSGRLEKTEDLWLRVRKDHA
  PRLARLSLESRSLQDVLLHRKPKLGQELGRGQYGVVYLCDNWGGHFPCALKSVVPPDEKHWNDLALEFHY
  MRSLPKHERLVDLHGSVIDYNYGGGSSIAVLLIMERLHRDLYTGLKAGLTLETRLQIALDVVEGIRFLHS
  QGLVHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTPIHMAPELFTGARPERLPVFDEECWQLM
  EACWDGDPLKRPLLGIVQPMLQGIMNRLCKSNSEQPNRGLDDST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199462
• ORF Size: 2652 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199462.1, NM_199462.2, NP_955749.1
• RefSeq Size: 7791 bp
• RefSeq ORF: 2655 bp
• Locus ID: 25778
• Protein Families: Druggable Genome, Protein Kinase
• MW: 100 kDa
• Gene Summary: This gene encodes a dual serine/threonine and tyrosine protein kinase which is expressed in multiple tissues. It is thought to function as a regulator of cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]