HIST1H4C (NM_003542) Human Tagged ORF Clone

CAT#: RC208854

HIST1H4C (Myc-DDK-tagged)-Human histone cluster 1, H4c (HIST1H4C)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIST1H4C
• Synonyms: dJ221C16.1; H4/g; H4FG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208854 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGTCGCGGCAAAGGCGGAAAAGGCTTGGGGAAGGGTGGTGCTAAGCGCCATCGTAAGGTGCTCC
  GGGATAACATCCAGGGCATTACAAAACCGGCTATTCGCCGTTTGGCTCGGCGCGGTGGCGTCAAGCGCAT
  TTCCGGTCTTATCTATGAGGAGACTCGAGGTGTGCTTAAGGTTTTCTTAGAGAACGTTATTCGAGACGCC
  GTCACCTATACGGAGCACGCCAAGCGCAAAACTGTCACAGCCATGGATGTAGTATATGCCCTAAAACGTC
  AGGGGCGCACTCTGTATGGCTTCGGCGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208854 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDA
  VTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003542
• ORF Size: 309 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003542.1, NM_003542.2, NM_003542.3, NP_003533.1
• RefSeq Size: 390
• RefSeq ORF: 312
• Locus ID: 8364
• Protein Pathways: Systemic lupus erythematosus
• MW: 11.4 kDa
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]