LRRC57 (NM_153260) Human Tagged ORF Clone

CAT#: RC208959

LRRC57 (Myc-DDK-tagged)-Human leucine rich repeat containing 57 (LRRC57)

Product Images

Coomassie blue staining of purified LRRC57 protein (Cat# TP308959). The protein was produced from HEK293T cells transfected with LRRC57 cDNA clone (Cat# RC208959) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRC57
• Synonyms: DKFZp686H1865; FLJ36812
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208959 representing NM_153260
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAACAGTGCGCTCCGCGCTCATGTGGAAACGGCGCAGAAAACTGGTGTCTTTCAGCTTAAGGACC
  GAGGGCTGACCGAGTTCCCCGCAGACTTGCAGAAGCTGACGAGCAATCTCAGGACCATCGACTTGTCCAA
  CAACAAGATCGAAAGCCTACCGCCTTTGCTGATAGGAAAGTTCACTCTGCTGAAGAGCCTCTCCCTGAAC
  AACAACAAACTGACTGTTCTGCCTGATGAGATATGCAATCTGAAAAAACTAGAGACGCTAAGCCTAAACA
  ACAATCACCTTAGAGAGCTGCCGTCTACCTTTGGGCAACTCTCTGCCCTCAAGACCCTGAGCCTCTCTGG
  GAACCAACTGGGAGCATTACCTCCCCAACTTTGTAGCCTACGGCACCTGGATGTGATGGATCTCTCTAAG
  AACCAGATTCGAAGTATACCTGACTCAGTGGGAGAGCTGCAAGTCATCGAACTCAACCTCAACCAGAATC
  AGATATCTCAGATCTCAGTGAAGATATCTTGCTGTCCACGCCTTAAAATTCTTCGCCTGGAAGAGAATTG
  TCTTGAGCTCAGCATGCTTCCCCAGAGCATCCTCAGTGATTCCCAGATCTGTCTGCTTGCTGTGGAAGGC
  AATCTTTTTGAAATAAAGAAACTTCGAGAACTGGAAGGCTATGATAAGTACATGGAGAGGTTCACAGCCA
  CCAAGAAGAAGTTTGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208959 representing NM_153260
  Red=Cloning site Green=Tags(s)
  
  MGNSALRAHVETAQKTGVFQLKDRGLTEFPADLQKLTSNLRTIDLSNNKIESLPPLLIGKFTLLKSLSLN
  NNKLTVLPDEICNLKKLETLSLNNNHLRELPSTFGQLSALKTLSLSGNQLGALPPQLCSLRHLDVMDLSK
  NQIRSIPDSVGELQVIELNLNQNQISQISVKISCCPRLKILRLEENCLELSMLPQSILSDSQICLLAVEG
  NLFEIKKLRELEGYDKYMERFTATKKKFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153260
• ORF Size: 717 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153260.1, NM_153260.2, NP_694992.1
• RefSeq Size: 2647
• RefSeq ORF: 720
• Locus ID: 255252
• Protein Families: Druggable Genome
• MW: 26.6 kDa