CSDC2 (NM_014460) Human Tagged ORF Clone

CAT#: RC208989

CSDC2 (Myc-DDK-tagged)-Human cold shock domain containing C2, RNA binding (CSDC2)

Product Images

Western blot validation of overexpression lysate (Cat# LY415263) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208989 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CSDC2 protein (Cat# TP308989). The protein was produced from HEK293T cells transfected with CSDC2 cDNA clone (Cat# RC208989) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSDC2
• Synonyms: dJ347H13.2; PIPPIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208989 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCAGAGTCGACGTCACCCCCAGTTGTGCCCCCGCTCCACTCCCCCAAGTCCCCAGTCTGGCCCA
  CCTTCCCCTTCCACAGGGAGGGCAGCAGGGTCTGGGAGCGGGGTGGTGTCCCACCTCGGGACCTACCCAG
  CCCTCTGCCCACCAAGCGGACCAGGACCTATTCAGCGACAGCCCGGGCCTCAGCTGGCCCCGTGTTCAAG
  GGCGTCTGTAAGCAGTTCTCACGCTCACAGGGCCATGGCTTCATCACCCCCGAGAACGGGTCCGAGGACA
  TCTTCGTACATGTGTCTGACATCGAGGGGGAGTACGTGCCAGTGGAGGGCGACGAGGTGACCTACAAGAT
  GTGCCCTATCCCTCCCAAGAACCAGAAGTTCCAGGCCGTGGAGGTGGTGCTCACTCAGCTGGCCCCCCAC
  ACTCCCCACGAGACGTGGTCTGGCCAGGTCGTGGGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208989 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTSESTSPPVVPPLHSPKSPVWPTFPFHREGSRVWERGGVPPRDLPSPLPTKRTRTYSATARASAGPVFK
  GVCKQFSRSQGHGFITPENGSEDIFVHVSDIEGEYVPVEGDEVTYKMCPIPPKNQKFQAVEVVLTQLAPH
  TPHETWSGQVVGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014460
• ORF Size: 459 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014460.1, NM_014460.2, NM_014460.3, NP_055275.1
• RefSeq Size: 2545 bp
• RefSeq ORF: 462 bp
• Locus ID: 27254
• Protein Families: Transcription Factors
• MW: 16.8 kDa