PATL1 (NM_152716) Human Tagged ORF Clone

CAT#: RC209002

PATL1 (Myc-DDK-tagged)-Human protein associated with topoisomerase II homolog 1 (yeast) (PATL1)

Product Images

Western blot validation of overexpression lysate (Cat# LY407336) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209002 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PATL1
• Synonyms: hPat1b; Pat1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209002 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTACAGTGTCTGTATTAGAATATGCTTTGCCTCAGAGGCCCCCCCAGGGTCCAGAAGATGATCGGG
  ACCTTTCTGAACGAGCATTACCAAGGCGGTCAACTTCACCTATCATTGGCAGTCCTCCTGTTAGAGCTGT
  CCCCATAGGCACCCCACCTAAACAGATGGCTGTACCCAGCTTCACCCAACAGATTCTGTGTCCGAAGCCT
  GTCCATGTTCGGCCCCCAATGCCACCTCGTTATCCTGCTCCCTATGGTGAGAGGATGTCTCCAAACCAGC
  TCTGCAGTGTCCCGAACTCTTCCCTCCTGGGTCACCCTTTTCCTCCTAGTGTTCCTCCTGTTCTCAGCCC
  CCTCCAGAGAGCACAGCTTCTTGGAGGAGCACAGCTACAGCCTGGACGGATGTCTCCCAGCCAGTTTGCA
  CGGGTCCCTGGATTTGTTGGTAGTCCACTTGCTGCCATGAATCCCAAGTTGCTACAAGGGCGAGTTGGGC
  AGATGCTTCCCCCAGCACCAGGCTTCCGTGCCTTCTTTAGTGCTCCACCCTCCGCTACACCACCTCCACA
  GCAGCACCCTCCTGGCCCAGGACCCCACCTGCAAAACCTAAGATCTCAGGCCCCAATGTTTAGACCGGAC
  ACAACTCACCTCCATCCACAGCACCGTCGACTCTTGCATCAGAGACAGCAACAGAATAGAAGTCAGCATC
  GGAATCTCAATGGTGCGGGAGATAGAGGAAGTCACCGGAGCAGTCATCAAGATCATCTCCGAAAGGATCC
  ATATGCCAATCTCATGTTGCAGCGGGAAAAGGATTGGGTCTCTAAAATCCAGATGATGCAACTGCAAAGC
  ACTGATCCCTACCTGGATGATTTTTATTACCAGAATTACTTTGAAAAACTGGAGAAACTGTCAGCTGCTG
  AAGAAATACAAGGTGATGGCCCTAAGAAGGAGCGCACCAAGCTTATCACCCCTCAGGTGGCCAAACTGGA
  GCACGCCTATAAGCCAGTGCAATTTGAGGGCTCTTTGGGAAAGCTTACCGTTTCTAGTGTGAATAATCCC
  CGAAAAATGATTGATGCTGTTGTGACATCTCGGAGTGAGGATGATGAGACAAAAGAAAAACAAGTTCGAG
  ACAAGAGGAGAAAAACCCTTGTTATAATTGAGAAAACCTACAGCTTACTCCTTGATGTGGAGGACTATGA
  AAGACGTTATCTCCTAAGTCTGGAAGAAGAGCGACCTGCCCTAATGGATGACAGAAAGCACAAAATTTGT
  AGCATGTATGACAACTTAAGGGGGAAATTGCCTGGACAAGAGAGGCCTAGTGATGACCACTTTGTACAGA
  TCATGTGTATCCGAAAAGGGAAGAGAATGGTTGCCCGTATTCTTCCTTTCCTCTCCACAGAGCAAGCAGC
  TGACATTCTCATGACAACAGCCAGGAACCTCCCTTTCCTTATCAAGAAGGATGCACAAGATGAGGTGCTG
  CCATGCTTACTGAGTCCCTTCTCTCTCCTTCTCTATCATCTTCCATCAGTGAGTATCACCAGCCTTTTGC
  GACAGCTAATGAACCTACCTCAAAGTGCAGCTACACCAGCACTCTCCAATCCTCACCTCACTGCTGTGCT
  CCAGAACAAGTTTGGCCTGTCACTGCTCCTCATCCTCCTGAGCCGTGGTGAAGACCTACAGAGTTCAGAC
  CCTGCTACAGAATCAACACAAAATAATCAGTGGACGGAGGTGATGTTCATGGCAACACGAGAACTTCTGC
  GGATTCCCCAAGCAGCCCTGGCCAAGCCAATCTCTATACCTACAAACCTAGTGTCCCTCTTTTCTCGCTA
  TGTTGACCGGCAGAAACTGAACTTGCTGGAGACAAAACTGCAGCTAGTTCAGGGGATACGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209002 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPTVSVLEYALPQRPPQGPEDDRDLSERALPRRSTSPIIGSPPVRAVPIGTPPKQMAVPSFTQQILCPKP
  VHVRPPMPPRYPAPYGERMSPNQLCSVPNSSLLGHPFPPSVPPVLSPLQRAQLLGGAQLQPGRMSPSQFA
  RVPGFVGSPLAAMNPKLLQGRVGQMLPPAPGFRAFFSAPPSATPPPQQHPPGPGPHLQNLRSQAPMFRPD
  TTHLHPQHRRLLHQRQQQNRSQHRNLNGAGDRGSHRSSHQDHLRKDPYANLMLQREKDWVSKIQMMQLQS
  TDPYLDDFYYQNYFEKLEKLSAAEEIQGDGPKKERTKLITPQVAKLEHAYKPVQFEGSLGKLTVSSVNNP
  RKMIDAVVTSRSEDDETKEKQVRDKRRKTLVIIEKTYSLLLDVEDYERRYLLSLEEERPALMDDRKHKIC
  SMYDNLRGKLPGQERPSDDHFVQIMCIRKGKRMVARILPFLSTEQAADILMTTARNLPFLIKKDAQDEVL
  PCLLSPFSLLLYHLPSVSITSLLRQLMNLPQSAATPALSNPHLTAVLQNKFGLSLLLILLSRGEDLQSSD
  PATESTQNNQWTEVMFMATRELLRIPQAALAKPISIPTNLVSLFSRYVDRQKLNLLETKLQLVQGIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152716
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152716.1, NP_689929.1
• RefSeq Size: 4142 bp
• RefSeq ORF: 2313 bp
• Locus ID: 219988
• Protein Pathways: RNA degradation
• MW: 70.6 kDa