KIF17 (NM_020816) Human Tagged ORF Clone

CAT#: RC209079

KIF17 (Myc-DDK-tagged)-Human kinesin family member 17 (KIF17), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412287) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209079 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF17
• Synonyms: KIF3X; KIF17B; KLP-2; OSM-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209079 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCGAGGCGGTGAAGGTTGTCGTGCGCTGCCGTCCCATGAACCAGCGGGAGCGAGAGCTGCGCT
  GCCAGCCCGTGGTGACTGTGGACTGCGCGCGCGCCCAGTGCTGCATCCAGAACCCGGGCGCCGCCGACGA
  GCCGCCCAAGCAGTTCACCTTCGACGGCGCCTACCACGTGGACCACGTCACCGAGCAGATCTACAACGAG
  ATCGCCTATCCGCTGGTGGAGGGCGTCACTGAGGGCTACAATGGCACCATCTTTGCCTACGGCCAGACAG
  GCAGCGGGAAGTCCTTCACCATGCAGGGCCTGCCGGATCCGCCCTCCCAGAGAGGCATCATCCCCAGGGC
  CTTCGAGCACGTGTTCGAGAGCGTCCAGTGTGCAGAGAACACTAAGTTCCTGGTCCGGGCCTCCTACCTG
  GAGATCTACAATGAAGATGTCCGGGACCTCCTTGGGGCTGACACCAAGCAGAAGCTGGAGCTGAAGGAGC
  ACCCAGAGAAGGGCGTGTACGTGAAGGGGCTGTCCATGCACACGGTGCACAGCGTGGCCCAGTGTGAGCA
  CATCATGGAGACTGGCTGGAAGAACCGTTCGGTCGGCTACACGCTGATGAACAAGGATTCCTCACGCTCG
  CACTCCATCTTCACCATCAGCATCGAGATGTCTGCCGTGGATGAGCGGGGCAAGGACCACCTCCGGGCGG
  GCAAGCTGAACCTGGTGGACCTGGCGGGCAGCGAGCGGCAGTCCAAGACCGGGGCCACGGGCGAGCGGCT
  CAAGGAGGCCACCAAGATCAACCTGTCGCTCTCGGCACTGGGCAATGTCATCTCGGCGCTGGTGGACGGG
  CGCTGTAAGCACGTCCCCTACCGTGACTCGAAGCTGACGCGGCTGCTGCAGGACTCACTGGGCGGCAACA
  CCAAGACGCTCATGGTGGCCTGCCTGTCGCCTGCGGACAACAACTACGATGAGACACTCAGCACGCTGCG
  CTACGCCAACCGGGCCAAGAACATCAGGAACAAGCCGCGCATCAATGAGGACCCCAAGGATGCGCTGCTT
  CGCGAGTACCAGGAGGAGATCAAGAAGCTCAAGGCCATCCTGACACAGCAGATGAGCCCCAGCAGCCTGT
  CAGCCCTGCTGTCCAGGCAGGTGCCCCCAGACCCTGTGCAGGTGGAGGAGAAGCTGTTGCCCCAACCTGT
  GATCCAGCATGACATGGAGGCCGAGAAGCAGCTGATCCGGGAGGAGTATGAAGAGCGCCTGGCCCGGCTG
  AAAGCCGACTATAAGGCCGAGCAGGAGTCTCGGGCCAGGCTGGAGGAAGACATCACTGCCATGCGCAACT
  CATATGACGTCAGGCTGTCCACGCTGGAGGAGAACCTGCGGAAGGAGACAGAGGCTGTCCTGCAGGTGGG
  AGTCCTCTACAAGGCTGAGGTCATGTCCAGGGCTGAGTTTGCCAGCAGCGCTGAGTACCCGCCTGCTTTT
  CAGTATGAGACAGTGGTGAAACCCAAGGTCTTCTCCACGACTGACACTCTGCCCAGTGACGATGTCTCCA
  AGACTCAGGTTTCCTCCAGGTTTGCGGAGCTGCCCAAGGTGGAACCCTCCAAATCTGAGATTTCTCTGGG
  CTCCAGTGAGTCATCCTCGCTCGAAGAAACCTCTGTGTCCGAGGCTTTCCCTGGGCCTGAGGAGCCCTCC
  AACGTGGAGGTCTCCATGCCCACTGAGGAGTCCAGGAGCAGATACTTCCTGGATGAGTGCCTCGGGCAGG
  AGGCCGCTGGGCACCTGCTGGGGGAACAGAACTACCTCCCGCAAGAGGAGCCGCAGGAGGTGCCCCTGCA
  GGGGTTACTAGGCCTGCAGGACCCGTTTGCCGAGGTGGAAGCCAAGCTGGCCAGACTCTCCTCCACCGTG
  GCCAGGACAGATGCACCCCAGGCAGACGTCCCCAAGGTCCCTGTGCAGGTCCCTGCGCCGACAGACCTGC
  TGGAGCCCAGTGATGCCAGGCCCGAAGCCGAGGCGGCTGATGACTTCCCGCCCAGGCCTGAGGTAGATCT
  GGCCTCGGAAGTGGCCTTAGAGGTGGTGCGGACAGCAGAGCCTGGCGTGTGGTTGGAGGCTCAGGCCCCG
  GTGGCCCTGGTGGCTCAGCCTGAGCCCCTGCCGGCCACAGCTGGTGTGAAGAGGGAGAGCGTGGGCATGG
  AGGTGGCAGTGCTGACTGATGACCCGCTGCCCGTTGTGGACCAGCAGCAGGTGCTGGCCCGTCTGCAGCT
  GTTGGAGCAGCAGGTTGTGGGTGGAGAGCAGGCCAAGAACAAGGACCTGAAGGAGAAGCACAAGCGGCGC
  AAGCGCTACGCAGACGAGCGCAGGAAGCAGCTGGTGGCTGCCCTGCAGAACTCGGATGAGGACAGCGGGG
  ACTGGGTGCTGCTTAACGTCTACGACTCCATCCAGGAGGAAGTGCGGGCCAAGAGCAAGCTGCTGGAGAA
  GATGCAGAGGAAGCTTCGGGCAGCAGAGGTGGAGATCAAAGATCTGCAGTCCGAGTTTCAGCTGGAGAAG
  ATCGATTACTTGGCCACCATCCGCCGGCAGGAGCGTGACTCCATGCTCTTGCAGCAGCTCCTGGAGCAGG
  TGCAGCCCCTGATTCGCAGGGACTGTAACTACAGCAACCTGGAGAAGATTCTGCGTGAGTCCTGCTGGGA
  CGAAGATAACGGCTTCTGGAAGATCCCACATCCCGTCATCACAAAAACCAGCCTCCCAGTAGTTTCAACT
  GGGCCACAGAACAAACCAGCCCGCAAAACCTCTGCAGCAGACAATGGCGAGCCGAACATGGAGGAGGACC
  GCTACAGGCTCATGCTCAGTCGGAGCAACAGTGAAAACATTGCCAGCAACTACTTCCGATCTAAGCGGGC
  CAGCCAGATCCTCAGCACAGACGCCAGGAAGAGCCTCACACATCACAACTCGCCACCAGGCCTCAGCTGC
  CCACTCAGCAACAACTCTGCCATCCCACCCACCCAGGCCCCTGAAATGCCCCAGCCCCGGCCCTTCCGCC
  TCGAGTCCCTCGACATCCCTTTCACCAAGGCCAAGCGTAAGAAAAGCAAAAGCAACTTTGGCAGTGAGCC
  TCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209079 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASEAVKVVVRCRPMNQRERELRCQPVVTVDCARAQCCIQNPGAADEPPKQFTFDGAYHVDHVTEQIYNE
  IAYPLVEGVTEGYNGTIFAYGQTGSGKSFTMQGLPDPPSQRGIIPRAFEHVFESVQCAENTKFLVRASYL
  EIYNEDVRDLLGADTKQKLELKEHPEKGVYVKGLSMHTVHSVAQCEHIMETGWKNRSVGYTLMNKDSSRS
  HSIFTISIEMSAVDERGKDHLRAGKLNLVDLAGSERQSKTGATGERLKEATKINLSLSALGNVISALVDG
  RCKHVPYRDSKLTRLLQDSLGGNTKTLMVACLSPADNNYDETLSTLRYANRAKNIRNKPRINEDPKDALL
  REYQEEIKKLKAILTQQMSPSSLSALLSRQVPPDPVQVEEKLLPQPVIQHDMEAEKQLIREEYEERLARL
  KADYKAEQESRARLEEDITAMRNSYDVRLSTLEENLRKETEAVLQVGVLYKAEVMSRAEFASSAEYPPAF
  QYETVVKPKVFSTTDTLPSDDVSKTQVSSRFAELPKVEPSKSEISLGSSESSSLEETSVSEAFPGPEEPS
  NVEVSMPTEESRSRYFLDECLGQEAAGHLLGEQNYLPQEEPQEVPLQGLLGLQDPFAEVEAKLARLSSTV
  ARTDAPQADVPKVPVQVPAPTDLLEPSDARPEAEAADDFPPRPEVDLASEVALEVVRTAEPGVWLEAQAP
  VALVAQPEPLPATAGVKRESVGMEVAVLTDDPLPVVDQQQVLARLQLLEQQVVGGEQAKNKDLKEKHKRR
  KRYADERRKQLVAALQNSDEDSGDWVLLNVYDSIQEEVRAKSKLLEKMQRKLRAAEVEIKDLQSEFQLEK
  IDYLATIRRQERDSMLLQQLLEQVQPLIRRDCNYSNLEKILRESCWDEDNGFWKIPHPVITKTSLPVVST
  GPQNKPARKTSAADNGEPNMEEDRYRLMLSRSNSENIASNYFRSKRASQILSTDARKSLTHHNSPPGLSC
  PLSNNSAIPPTQAPEMPQPRPFRLESLDIPFTKAKRKKSKSNFGSEPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020816
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020816.1, NM_020816.2, NM_020816.3, NP_065867.1
• RefSeq Size: 4003 bp
• RefSeq ORF: 3090 bp
• Locus ID: 57576
• Protein Families: Druggable Genome
• MW: 115 kDa