SSPN (NM_005086) Human Tagged ORF Clone

CAT#: RC209123

SSPN (Myc-DDK-tagged)-Human sarcospan (Kras oncogene-associated gene) (SSPN), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417535) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209123 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SSPN protein (Cat# TP309123). The protein was produced from HEK293T cells transfected with SSPN cDNA clone (Cat# RC209123) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SSPN
• Synonyms: DAGA5; KRAG; NSPN; SPN1; SPN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209123 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAACAAGCAGCCACGCGGCCAGCAGAGGCAGGGGGGCCCGCCGGCCGCGGACGCCGCTGGGC
  CCGACGACATGGAGCCGAAGAAGGGCACGGGGGCCCCCAAGGAGTGCGGGGAGGAGGAGCCCCGGACCTG
  CTGCGGCTGCCGGTTCCCGCTGCTGCTCGCCCTGCTGCAGCTGGCCCTGGGCATCGCCGTGACCGTGGTG
  GGCTTCCTCATGGCGAGCATCAGCTCCTCCCTGCTAGTCAGGGACACTCCATTTTGGGCTGGGATCATTG
  TCTGCTTAGTGGCCTATCTTGGCTTGTTTATGCTTTGTGTCTCATATCAGGTTGACGAACGGACATGTAT
  TCAATTTTCTATGAAACTGTTATACTTTCTGCTGAGTGCCCTGGGCCTGACGGTCTGTGTGCTGGCCGTG
  GCCTTTGCCGCCCACCACTATTCGCAGCTCACACAGTTTACCTGTGAGACCACACTCGACTCTTGCCAGT
  GCAAACTGCCCTCCTCGGAGCCGCTCAGCAGGACCTTTGTTTACCGGGATGTGACGGACTGTACCAGCGT
  CACTGGCACTTTCAAACTGTTCTTACTCATCCAGATGATTCTTAATTTGGTCTGCGGCCTTGTGTGCTTG
  TTGGCCTGCTTTGTGATGTGGAAACATAGGTACCAGGTCTTCTATGTGGGTGTCAGGATATGCTCCCTCA
  CGGCTTCCGAAGGCCCCCAGCAAAAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209123 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKNKQPRGQQRQGGPPAADAAGPDDMEPKKGTGAPKECGEEEPRTCCGCRFPLLLALLQLALGIAVTVV
  GFLMASISSSLLVRDTPFWAGIIVCLVAYLGLFMLCVSYQVDERTCIQFSMKLLYFLLSALGLTVCVLAV
  AFAAHHYSQLTQFTCETTLDSCQCKLPSSEPLSRTFVYRDVTDCTSVTGTFKLFLLIQMILNLVCGLVCL
  LACFVMWKHRYQVFYVGVRICSLTASEGPQQKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005086
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005086.3, NM_005086.4, NP_005077.2
• RefSeq Size: 4547
• RefSeq ORF: 732
• Locus ID: 8082
• Protein Families: Druggable Genome, Transmembrane
• MW: 26.6 kDa
• Gene Summary: This gene encodes a member of the dystrophin-glycoprotein complex (DGC). The DGC spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Two alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]