PEX13 (NM_002618) Human Tagged ORF Clone

CAT#: RC209140

PEX13 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 13 (PEX13)

Product Images

Western blot validation of overexpression lysate (Cat# LY419218) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209140 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PEX13
• Synonyms: NALD; PBD11A; PBD11B; ZWS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209140 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCCAGCCGCCACCTCCCCCCAAACCCTGGGAGACCCGCCGAATTCCGGGAGCCGGACCGGGAC
  CAGGACCGGGCCCCACTTTCCAATCTGCTGATTTGGGTCCTACTTTAATGACAAGACCTGGACAACCAGC
  ACTTACCAGAGTGCCCCCACCTATTCTTCCAAGGCCATCACAGCAGACAGGAAGTAGCAGTGTGAACACT
  TTTAGACCTGCTTACAGTTCATTTTCTTCTGGATATGGTGCCTATGGAAATTCATTTTATGGAGGCTATA
  GTCCTTATAGTTATGGATATAATGGGCTGGGCTACAACCGCCTCCGTGTAGATGATCTTCCACCCAGTAG
  ATTTGTTCAGCAAGCTGAAGAAAGCAGCAGGGGTGCATTTCAGTCCATTGAAAGTATTGTGCATGCATTT
  GCCTCTGTCAGTATGATGATGGATGCTACCTTTTCAGCTGTCTATAACAGTTTCAGGGCTGTATTGGATG
  TAGCAAATCACTTTTCCCGATTGAAAATACACTTTACAAAAGTGTTTTCAGCTTTTGCATTGGTTAGGAC
  TATACGGTATCTTTACAGACGGCTACAGCGGATGTTAGGTTTAAGAAGAGGCTCTGAGAATGAAGACCTC
  TGGGCAGAGAGTGAAGGAACTGTGGCATGCCTTGGTGCTGAGGACCGAGCAGCTACCTCAGCAAAATCTT
  GGCCAATATTCTTGTTCTTTGCTGTTATCCTTGGTGGTCCTTACCTCATTTGGAAACTATTGTCTACTCA
  CAGTGATGAAGTAACAGACAGCATCAACTGGGCAAGTGGTGAGGATGACCATGTAGTTGCCAGAGCAGAA
  TATGATTTTGCTGCCGTATCTGAAGAAGAAATTTCTTTCCGGGCTGGTGATATGCTGAACTTAGCTCTCA
  AAGAACAACAACCCAAAGTGCGTGGTTGGCTTCTGGCTAGCCTTGATGGCCAAACAACAGGACTTATACC
  TGCGAATTATGTCAAAATTCTTGGCAAAAGAAAAGGTAGGAAAACGGTGGAATCAAGTAAAGTTTCCAAG
  CAGCAACAATCTTTTACCAACCCAACACTAACTAAAGGAGCCACGGTTGCTGATTCTTTGGATGAACAGG
  AAGCTGCCTTTGAATCTGTTTTTGTTGAAACTAATAAGGTTCCAGTTGCACCTGATTCCATTGGGAAAGA
  TGGAGAAAAGCAAGATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209140 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASQPPPPPKPWETRRIPGAGPGPGPGPTFQSADLGPTLMTRPGQPALTRVPPPILPRPSQQTGSSSVNT
  FRPAYSSFSSGYGAYGNSFYGGYSPYSYGYNGLGYNRLRVDDLPPSRFVQQAEESSRGAFQSIESIVHAF
  ASVSMMMDATFSAVYNSFRAVLDVANHFSRLKIHFTKVFSAFALVRTIRYLYRRLQRMLGLRRGSENEDL
  WAESEGTVACLGAEDRAATSAKSWPIFLFFAVILGGPYLIWKLLSTHSDEVTDSINWASGEDDHVVARAE
  YDFAAVSEEEISFRAGDMLNLALKEQQPKVRGWLLASLDGQTTGLIPANYVKILGKRKGRKTVESSKVSK
  QQQSFTNPTLTKGATVADSLDEQEAAFESVFVETNKVPVAPDSIGKDGEKQDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002618
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002618.1, NM_002618.2, NM_002618.3, NP_002609.1
• RefSeq Size: 4524 bp
• RefSeq ORF: 1212 bp
• Locus ID: 5194
• Cytogenetics: 2p15
• Domains: SH3, Peroxin-13_N
• Protein Families: Druggable Genome
• MW: 44.1 kDa
• Gene Summary: 'This gene encodes a peroxisomal membrane protein that binds the type 1 peroxisomal targeting signal receptor via a SH3 domain located in the cytoplasm. Mutations and deficiencies in peroxisomal protein importing and peroxisome assembly lead to peroxisomal biogenesis disorders, an example of which is Zellweger syndrome. [provided by RefSeq, Oct 2008]'