ANKRD16 (NM_019046) Human Tagged ORF Clone

CAT#: RC209157

ANKRD16 (Myc-DDK-tagged)-Human ankyrin repeat domain 16 (ANKRD16), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY423159) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215131 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ANKRD16 protein (Cat# TP309157). The protein was produced from HEK293T cells transfected with ANKRD16 cDNA clone (Cat# RC209157) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKRD16
• Synonyms: ankyrin repeat domain 16; OTTHUMP00000019019; OTTHUMP00000044872
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209157 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCCCGGGGACCCGCGGCGCCTCTGCAGGCTGGTGCAGGAGGGCCGGCTGCGCGCCCTGAAGG
  AGGAGCTGCAGGCGGCCGGGGGCTGCCCGGGGCCGGCCGGGGATACCCTCCTGCACTGCGCCGCGCGCCA
  CGGGCATCGGGACGTGCTGGCCTATCTGGCCGAGGCCTGGGGCATGGACATCGAGGCCACCAACCGAGAC
  TACAAGCGGCCTCTGCACGAGGCGGCCTCCATGGGCCACCGAGACTGCGTGCGCTACCTGCTGGGCCGGG
  GGGCAGCGGTCGACTGCCTGAAGAAGGCCGACTGGACTCCTCTGATGATGGCCTGCACAAGGAAGAACCT
  GGGGGTGATCCAGGAGCTGGTGGAACATGGCGCCAATCCACTCCTGAAGAACAAAGATGGCTGGAACAGT
  TTCCACATTGCCAGTCGAGAAGGCGACCCTCTGATCCTCCAGTACCTGCTCACTGTTTGCCCAGGTGCCT
  GGAAGACAGAGAGCAAAATTAGAAGGACTCCTCTGCATACTGCAGCAATGCATGGCCATTTGGAGGCAGT
  CAAGGTGCTTCTTAAGAGGTGCCAATATGAACCAGACTACAGAGACAACTGTGGCGTCACCGCCTTGATG
  GACGCAATCCAGTGTGGGCACATCGACGTCGCTAGGCTGCTCCTCGATGAACATGGGGCTTGCCTTTCAG
  CAGAAGACAGCCTGGGTGCCCAGGCTCTGCACAGGGCAGCTGTCACAGGGCAGGACGAAGCCATCCGATT
  CTTGGTCTCTGAACTTGGCGTCGATGTAGATGTGAGAGCCACATCAACCCACCTCACAGCACTTCATTAT
  GCAGCTAAGGAAGGACATACAAGTACAATTCAGACTCTCTTATCCTTGGGAGCTGACATCAATTCTAAAG
  ATGAAAAAAATCGATCAGCCCTGCATCTGGCCTGTGCAGGTCAGCACTTGGCCTGTGCCAAGTTTCTCCT
  GCAGTCGGGACTGAAGGATTCTGAAGACATCACGGGCACCCTGGCTCAGCAGCTCCCAAGGAGAGCAGAT
  GTCCTTCAGGGCTCTGGCCATAGCGCAATGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209157 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQPGDPRRLCRLVQEGRLRALKEELQAAGGCPGPAGDTLLHCAARHGHRDVLAYLAEAWGMDIEATNRD
  YKRPLHEAASMGHRDCVRYLLGRGAAVDCLKKADWTPLMMACTRKNLGVIQELVEHGANPLLKNKDGWNS
  FHIASREGDPLILQYLLTVCPGAWKTESKIRRTPLHTAAMHGHLEAVKVLLKRCQYEPDYRDNCGVTALM
  DAIQCGHIDVARLLLDEHGACLSAEDSLGAQALHRAAVTGQDEAIRFLVSELGVDVDVRATSTHLTALHY
  AAKEGHTSTIQTLLSLGADINSKDEKNRSALHLACAGQHLACAKFLLQSGLKDSEDITGTLAQQLPRRAD
  VLQGSGHSAMT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019046
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019046.1, NM_019046.2, NP_061919.1
• RefSeq Size: 2646
• RefSeq ORF: 1086
• Locus ID: 54522
• MW: 39.3 kDa