AGTPBP1 (NM_015239) Human Tagged ORF Clone

CAT#: RC209238

AGTPBP1 (Myc-DDK-tagged)-Human ATP/GTP binding protein 1 (AGTPBP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY414709) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209238 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified AGTPBP1 protein (Cat# TP309238). The protein was produced from HEK293T cells transfected with AGTPBP1 cDNA clone (Cat# RC209238) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AGTPBP1
• Synonyms: CCP1; CONDCA; NNA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209238 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAGTTAAAAGTGATACCAGAAAAAAGCCTTACCAATAATTCTAGGATCGTAGGACTCCTGGCTC
  AACTGGAGAAGATCAATGCTGAGCCTTCAGAATCAGACACTGCCCGATATGTTACATCAAAAATTCTTCA
  TCTGGCTCAGAGTCAAGAAAAAACAAGGAGAGAAATGACAGCCAAAGGTTCTACAGGAATGGAAATTCTG
  CTGTCAACATTAGAGAACACAAAAGATCTTCAAACTACACTTAATATCTTAAGCATTCTTGTTGAGCTGG
  TGTCAGCTGGTGGAGGTCGAAGAGTGAGTTTCTTAGTCACCAAAGGTGGTTCACAAATATTGTTGCAGTT
  ACTTATGAATGCCAGCAAAGAATCTCCCCCACATGAGGACTTAATGGTACAGATTCATTCTATTCTTGCA
  AAGATTGGACCAAAAGATAAAAAATTTGGAGTAAAGGCTAGAATTAATGGGGCTCTGAATATAACCCTGA
  ATTTGGTCAAGCAGAATTTGCAGAATCATCGCTTGGTTCTACCTTGCCTTCAGCTTTTACGAGTATATTC
  TGCCAACTCTGTGAATTCAGTATCCTTAGGGAAAAATGGAGTTGTGGAACTGATGTTTAAAATCATTGGA
  CCATTTAGTAAGAAGAATTCCAGTCTTATAAAGGTTGCTTTAGACACTCTTGCTGCATTGCTAAAATCAA
  AAACAAATGCCAGGAGAGCTGTAGACAGAGGATATGTCCAAGTGCTTTTAACAATTTATGTAGATTGGCA
  CCGCCATGATAACCGGCATAGAAACATGCTCATTCGGAAAGGAATTTTACAGAGTTTAAAAAGTGTTACA
  AACATCAAGTTGGGAAGAAAAGCATTTATTGATGCCAATGGGATGAAAATTCTGTATAATACTTCGCAAT
  TGCCTGTTATTCCTGTGACTGGTCCTGTGGCTCAGCTCTACAGCTTACCTCCTGAAGTGGATGACGTAGT
  AGATGAAAGTGATGACAACGATGATATTGATGTAGAAGCTGAAAACGAAACTGAGAATGAAGATGACCTA
  GATCAAAATTTTAAGAATGATGATATTGAAACAGATATTAACAAACTAAAACCCCAGCAAGAACCGGGAC
  GAACAATAGAAGATCTAAAAATGTATGAACACCTTTTCCCTGAGCTTGTTGATGATTTTCAGGACTATGA
  TTTAATCTCCAAAGAACCAAAGCCTTTTGTATTTGAGGGAAAAGTACGTGGTCCTATTGTTGTTCCTACG
  GCAGGCGAGGAAACATCTGGGAATTCTGGCAATTTAAGAAAAGTTGTAATGAAGGAGAACATATCTTCTA
  AAGGAGATGAAGGTGAAAAGAAGTCTACCTTTATGGATCTAGCAAAAGAAGATATTAAAGATAATGATAG
  AACATTACAACAGCAGCCAGGTGATCAAAATAGAACTATTTCATCAGTCCATGGTTTAAACAATGATATT
  GTAAAGGCCTTGGACCGAATTACATTGCAGAATATTCCTTCTCAAACAGCCCCAGGTTTTACTGCAGAAA
  TGAAGAAGGACTGCAGTCTTCCTCTTACTGTCCTTACCTGTGCTAAAGCATGTCCACACATGGCTACTTG
  TGGAAATGTTCTGTTTGAGGGAAGAACAGTTCAGCTAGGGAAGCTTTGCTGCACTGGAGTTGAAACTGAA
  GATGATGAAGATACTGAGTCAAATTCATCGGTAGAACAAGCATCGGTTGAAGTACCTGATGGACCAACAC
  TCCATGACCCAGACCTCTATATTGAGATTGTGAAAAATACGAAGTCTGTCCCAGAATATTCAGAGGTGGC
  TTATCCCGATTATTTTGGTCACATTCCGCCTCCATTCAAAGAGCCTATTTTAGAAAGGCCTTATGGTGTA
  CAAAGGACAAAAATTGCTCAAGATATTGAAAGGCTAATACATCAGAGTGATATCATAGATCGTGTGGTAT
  ATGACTTGGATAACCCAAATTACACCATTCCAGAAGAGGGAGATATTTTGAAATTTAACTCCAAATTTGA
  GTCTGGGAATCTGCGCAAAGTAATTCAAATTAGAAAAAATGAATATGATCTTATTCTGAACTCAGACATA
  AACAGCAATCATTATCATCAGTGGTTTTACTTTGAAGTCAGTGGAATGCGACCAGGTGTTGCTTACAGGT
  TTAACATCATTAACTGTGAAAAGTCCAACAGTCAGTTTAATTATGGTATGCAACCACTCATGTATTCGGT
  TCAGGAAGCATTAAATGCCAGACCATGGTGGATTCGTATGGGGACTGACATTTGTTACTATAAAAATCAT
  TTCTCAAGAAGTTCAGTTGCTGCAGGTGGGCAAAAGGGAAAATCCTACTATACAATTACATTTACTGTCA
  ATTTTCCACATAAAGATGATGTTTGCTACTTTGCTTATCACTATCCATATACGTATTCAACTTTACAGAT
  GCATCTTCAAAAATTGGAATCAGCACACAATCCTCAGCAAATCTATTTTCGGAAAGATGTGTTATGTGAA
  ACCCTGTCTGGAAACAGCTGCCCCTTGGTGACTATAACAGCAATGCCAGAGTCTAATTATTATGAACATA
  TCTGCCATTTCAGAAATCGCCCTTACGTTTTCTTGTCTGCTCGGGTACATCCTGGAGAAACTAATGCAAG
  TTGGGTTATGAAAGGAACGTTGGAATATCTCATGAGCAATAACCCCACTGCTCAGAGCTTACGAGAATCT
  TATATTTTTAAAATTGTCCCTATGTTAAATCCAGATGGTGTCATCAATGGAAATCATCGCTGTTCTTTAA
  GTGGAGAGGATTTGAATAGGCAGTGGCAAAGTCCAAGTCCGGATTTACATCCTACAATTTACCATGCTAA
  GGGGCTGTTGCAATACTTGGCTGCAGTGAAGCGTTTACCCTTGGTTTATTGTGATTATCATGGCCATTCC
  CGAAAGAAGAATGTATTTATGTATGGTTGCAGCATCAAAGAGACAGTGTGGCATACCAATGATAATGCAA
  CTTCATGTGATGTTGTGGAGGATACGGGATACAGGACATTGCCTAAGATACTGAGCCATATCGCCCCAGC
  ATTTTGCATGAGCAGCTGTAGCTTCGTAGTGGAAAAATCTAAAGAATCCACAGCACGTGTTGTAGTTTGG
  AGGGAAATAGGAGTACAAAGAAGTTATACCATGGAGAGTACTTTATGTGGCTGTGATCAGGGAAAATACA
  AGGGTTTACAGATTGGTACCCGAGAACTGGAAGAGATGGGAGCAAAATTTTGTGTTGGTCTTTTACGTTT
  GAAAAGACTGACCTCTCCATTGGAGTATAATCTGCCTTCCAGCCTGCTTGACTTTGAAAATGATTTAATT
  GAATCAAGCTGCAAAGTAACTAGCCCTACCACTTATGTCTTGGATGAAGATGAACCTCGATTCCTTGAAG
  AAGTTGATTACAGTGCAGAAAGTAATGATGAGTTAGATATTGAGTTGGCTGAAAATGTAGGAGATTATGA
  ACCTTCTGCTCAAGAAGAAGTACTTTCTGACTCTGAATTATCAAGAACATACCTACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209238 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKLKVIPEKSLTNNSRIVGLLAQLEKINAEPSESDTARYVTSKILHLAQSQEKTRREMTAKGSTGMEIL
  LSTLENTKDLQTTLNILSILVELVSAGGGRRVSFLVTKGGSQILLQLLMNASKESPPHEDLMVQIHSILA
  KIGPKDKKFGVKARINGALNITLNLVKQNLQNHRLVLPCLQLLRVYSANSVNSVSLGKNGVVELMFKIIG
  PFSKKNSSLIKVALDTLAALLKSKTNARRAVDRGYVQVLLTIYVDWHRHDNRHRNMLIRKGILQSLKSVT
  NIKLGRKAFIDANGMKILYNTSQLPVIPVTGPVAQLYSLPPEVDDVVDESDDNDDIDVEAENETENEDDL
  DQNFKNDDIETDINKLKPQQEPGRTIEDLKMYEHLFPELVDDFQDYDLISKEPKPFVFEGKVRGPIVVPT
  AGEETSGNSGNLRKVVMKENISSKGDEGEKKSTFMDLAKEDIKDNDRTLQQQPGDQNRTISSVHGLNNDI
  VKALDRITLQNIPSQTAPGFTAEMKKDCSLPLTVLTCAKACPHMATCGNVLFEGRTVQLGKLCCTGVETE
  DDEDTESNSSVEQASVEVPDGPTLHDPDLYIEIVKNTKSVPEYSEVAYPDYFGHIPPPFKEPILERPYGV
  QRTKIAQDIERLIHQSDIIDRVVYDLDNPNYTIPEEGDILKFNSKFESGNLRKVIQIRKNEYDLILNSDI
  NSNHYHQWFYFEVSGMRPGVAYRFNIINCEKSNSQFNYGMQPLMYSVQEALNARPWWIRMGTDICYYKNH
  FSRSSVAAGGQKGKSYYTITFTVNFPHKDDVCYFAYHYPYTYSTLQMHLQKLESAHNPQQIYFRKDVLCE
  TLSGNSCPLVTITAMPESNYYEHICHFRNRPYVFLSARVHPGETNASWVMKGTLEYLMSNNPTAQSLRES
  YIFKIVPMLNPDGVINGNHRCSLSGEDLNRQWQSPSPDLHPTIYHAKGLLQYLAAVKRLPLVYCDYHGHS
  RKKNVFMYGCSIKETVWHTNDNATSCDVVEDTGYRTLPKILSHIAPAFCMSSCSFVVEKSKESTARVVVW
  REIGVQRSYTMESTLCGCDQGKYKGLQIGTRELEEMGAKFCVGLLRLKRLTSPLEYNLPSSLLDFENDLI
  ESSCKVTSPTTYVLDEDEPRFLEEVDYSAESNDELDIELAENVGDYEPSAQEEVLSDSELSRTYLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015239
• ORF Size: 3558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015239.1, NM_015239.2, NP_056054.1
• RefSeq Size: 4436 bp
• RefSeq ORF: 3561 bp
• Locus ID: 23287
• Domains: Zn_carbOpept
• Protein Families: Protease
• MW: 133.9 kDa
• Gene Summary: NNA1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motif that was initially cloned from regenerating spinal cord neurons of the mouse. [supplied by OMIM, Jul 2002]