PRPF8 (NM_006445) Human Tagged ORF Clone
CAT#: RC209472
- TrueORF®
PRPF8 (Myc-DDK-tagged)-Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8)
"NM_006445" in other vectors (3)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PRPF8 |
Synonyms | HPRP8; PRP8; PRPC8; RP13; SNRNP220 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209472 representing NM_006445
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGTGTTTCCTTATCGAGGGCCGGGTAACCCGGTGCCTGGCCCTCTAGCCCCGCTACCGGACT ACATGTCGGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAATTGCAGGCCAAGCGCTATGCAGA AAAGCGGAAGTTTGGGTTTGTGGATGCCCAGAAGGAAGACATGCCCCCAGAACATGTCAGGAAGATCATT CGAGACCATGGAGACATGACCAACAGGAAGTTCCGCCATGACAAAAGGGTTTACTTGGGTGCCCTAAAGT ACATGCCCCACGCAGTCCTCAAACTCCTGGAGAACATGCCTATGCCTTGGGAGCAGATTCGGGATGTGCC TGTGCTGTACCACATCACTGGAGCCATTTCCTTCGTCAATGAGATTCCCTGGGTCATTGAACCTGTCTAC ATCTCCCAGTGGGGGTCAATGTGGATTATGATGCGCCGAGAAAAAAGAGATAGGAGGCATTTCAAGAGGA TGCGTTTTCCCCCTTTTGATGATGAGGAGCCGCCCTTGGACTATGCTGACAACATCCTAGATGTTGAGCC ACTGGAGGCCATTCAGCTAGAGCTGGACCCTGAGGAGGACGCCCCTGTGTTGGACTGGTTCTATGACCAC CAGCCGTTGAGGGACAGCAGGAAGTATGTAAATGGCTCCACTTACCAGCGCTGGCAGTTCACACTACCTA TGATGTCGACTCTCTACCGCCTGGCTAATCAGCTCCTGACAGACTTGGTGGATGACAACTACTTCTACCT GTTTGATTTGAAGGCCTTCTTTACGTCCAAGGCACTCAATATGGCCATTCCTGGAGGCCCCAAATTTGAA CCTCTTGTTCGAGACATCAACCTACAGGATGAAGACTGGAATGAATTCAATGATATTAACAAGATTATCA TCCGGCAGCCTATCCGCACTGAGTACAAGATTGCTTTTCCTTACTTGTACAACAATCTTCCACACCATGT CCACCTCACCTGGTACCATACTCCCAATGTTGTATTCATCAAAACTGAGGATCCTGACTTGCCAGCTTTC TACTTTGACCCTTTGATCAACCCAATCTCCCATAGGCACTCAGTCAAGAGCCAGGAACCATTGCCGGATG ATGATGAGGAATTTGAGCTCCCGGAGTTTGTGGAGCCCTTCCTGAAGGACACACCCCTCTATACAGACAA TACAGCCAATGGCATTGCCCTGCTCTGGGCCCCGCGGCCCTTCAACCTACGCTCTGGTCGCACCCGTCGG GCCCTGGACATACCCCTTGTCAAGAACTGGTATCGGGAGCATTGTCCTGCCGGGCAGCCTGTGAAAGTGA GGGTCTCCTACCAGAAGCTGCTTAAGTACTATGTGCTGAATGCCCTGAAGCATCGGCCCCCTAAGGCTCA AAAGAAGAGGTATTTGTTCCGCTCCTTCAAAGCCACCAAATTCTTTCAGTCCACAAAGCTGGACTGGGTG GAGGTTGGGCTCCAGGTTTGCCGCCAGGGCTACAACATGCTCAACCTTCTCATTCACCGCAAAAACCTCA ACTACCTGCACCTGGACTACAACTTCAACCTCAAGCCTGTGAAAACGCTCACCACCAAGGAAAGAAAGAA ATCTCGTTTTGGGAATGCTTTCCACCTGTGTCGGGAAGTTCTGCGTTTGACTAAGCTGGTGGTGGATAGT CACGTGCAGTATCGGCTGGGCAATGTGGATGCCTTCCAGCTGGCAGATGGATTGCAGTATATATTTGCCC ATGTTGGGCAGTTGACGGGCATGTATCGATACAAATACAAGCTGATGCGACAGATTCGCATGTGCAAGGA CCTGAAGCATCTCATCTATTATCGTTTCAACACGGGCCCTGTAGGGAAGGGTCCTGGCTGTGGCTTCTGG GCTGCCGGTTGGCGAGTCTGGCTCTTTTTCATGCGTGGCATTACCCCTTTATTAGAGCGATGGCTTGGCA ACCTCCTGGCCCGGCAGTTTGAAGGTCGACACTCAAAGGGGGTGGCAAAGACAGTAACAAAGCAGCGAGT GGAGTCACATTTTGACCTTGAGCTGCGGGCAGCTGTGATGCATGATATTCTGGACATGATGCCTGAGGGG ATCAAGCAGAACAAGGCCCGGACAATCCTGCAGCACCTCAGTGAAGCCTGGCGCTGCTGGAAAGCCAACA TTCCCTGGAAGGTCCCTGGGCTGCCGACGCCCATAGAGAATATGATCCTTCGATACGTGAAGGCCAAGGC TGACTGGTGGACCAACACTGCCCACTACAACCGAGAACGGATCCGCCGAGGGGCCACTGTGGACAAGACT GTTTGTAAAAAGAATCTGGGCCGCCTCACCCGGCTCTATCTGAAGGCAGAACAGGAGCGGCAGCACAACT ACCTGAAGGACGGGCCTTACATCACAGCGGAGGAAGCAGTGGCAGTATATACCACCACAGTGCATTGGTT GGAAAGCCGCAGGTTTTCACCCATCCCATTCCCCCCACTCTCCTATAAGCATGACACCAAGTTGCTCATC TTGGCATTGGAGCGGCTCAAGGAAGCTTATAGTGTGAAGTCTCGGTTGAACCAGTCTCAGAGGGAGGAGC TAGGTCTGATCGAGCAGGCCTACGATAACCCCCACGAGGCGCTGTCCCGCATCAAGCGTCACCTCCTCAC ACAGAGAGCCTTCAAAGAGGTGGGCATTGAGTTCATGGATCTGTATAGCCACCTCGTTCCAGTATATGAT GTTGAGCCCCTGGAGAAGATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCCGACAAGCGCC GCCTGTTCCCACCCTGGATTAAGCCTGCAGACACAGAACCACCTCCGCTGCTTGTTTACAAGTGGTGTCA AGGCATCAATAACCTGCAGGACGTGTGGGAGACGAGTGAAGGCGAGTGCAATGTCATGCTGGAATCCCGC TTTGAGAAGATGTATGAGAAGATCGACTTGACTCTGCTCAACAGGCTGCTGCGCCTCATCGTGGACCACA ACATAGCCGACTACATGACAGCCAAGAACAACGTCGTCATCAACTATAAGGACATGAACCATACGAATTC ATATGGGATCATCAGAGGCCTGCAGTTTGCCTCATTCATCGTGCAGTATTATGGCCTGGTGATGGATTTG CTTGTATTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCCCCTCAGATGCCAAATGACTTTCTCAGTT TCCAGGACATAGCCACTGAGGCTGCCCACCCCATCCGTCTCTTCTGCAGATACATTGATCGCATCCATAT TTTTTTCAGGTTCACAGCAGATGAGGCTCGGGACCTGATTCAACGTTACCTGACAGAGCACCCTGACCCC AATAATGAAAACATCGTTGGCTATAATAACAAGAAGTGCTGGCCCCGAGATGCCCGCATGCGCCTCATGA AACATGATGTTAACTTAGGCCGGGCGGTATTCTGGGACATCAAGAACCGCTTGCCACGGTCAGTGACTAC AGTTCAGTGGGAGAACAGCTTCGTGTCTGTGTACAGTAAGGACAACCCCAACCTGCTGTTCAACATGTGT GGCTTCGAGTGCCGCATCCTGCCTAAGTGCCGCACCAGCTATGAGGAGTTCACCCACAAGGACGGGGTCT GGAACCTGCAGAATGAGGTTACTAAGGAGCGCACAGCTCAGTGTTTCCTGCGTGTGGACGATGAGTCAAT GCAGCGCTTCCACAACCGCGTGCGTCAGATTCTCATGGCCTCTGGGTCCACCACCTTCACCAAGATTGTG AATAAGTGGAATACAGCTCTCATTGGCCTTATGACATACTTTCGGGAGGCTGTGGTGAACACCCAAGAGC TCTTGGACTTACTGGTGAAGTGTGAGAACAAAATCCAGACACGTATCAAGATTGGACTCAACTCCAAGAT GCCAAGTCGGTTCCCCCCGGTTGTGTTCTACACCCCTAAGGAGTTGGGTGGACTCGGCATGCTCTCAATG GGCCATGTGCTCATCCCCCAATCCGACCTCAGGTGGTCCAAACAGACAGATGTAGGTATCACACACTTTC GTTCAGGAATGAGCCATGAAGAAGACCAGCTCATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAG CGAGTTCATTGATTCTCAGCGGGTCTGGGCTGAGTACGCACTCAAGAGGCAAGAGGCCATTGCTCAGAAC AGACGCCTGACTTTAGAAGACCTAGAAGATTCATGGGATCGTGGCATTCCTCGAATCAATACCCTCTTCC AGAAGGACCGGCACACACTGGCTTATGATAAGGGCTGGCGTGTCAGAACTGACTTTAAGCAGTATCAGGT TTTGAAGCAGAATCCGTTCTGGTGGACACACCAGCGGCATGATGGGAAGCTCTGGAACCTGAACAACTAC CGTACAGACATGATCCAGGCCCTGGGCGGTGTGGAAGGCATTCTGGAACACACACTCTTTAAGGGCACTT ACTTCCCTACCTGGGAGGGGCTTTTCTGGGAGAAGGCCAGTGGCTTTGAGGAATCTATGAAGTGGAAGAA GCTAACTAATGCTCAGCGATCAGGACTGAACCAGATTCCCAATCGTAGATTCACCCTCTGGTGGTCCCCG ACCATTAATCGAGCCAATGTATATGTAGGCTTTCAGGTGCAGCTAGACCTGACGGGTATCTTCATGCACG GCAAGATCCCCACGCTGAAGATCTCTCTCATCCAGATCTTCCGAGCTCACTTGTGGCAGAAGATCCATGA GAGCATTGTTATGGACTTATGTCAGGTGTTTGACCAGGAACTTGATGCACTGGAAATTGAGACAGTACAA AAGGAGACAATCCATCCCCGAAAGTCATATAAGATGAACTCTTCCTGTGCAGATATCCTGCTCTTTGCCT CCTATAAGTGGAATGTCTCCCGGCCCTCATTGCTGGCTGACTCCAAGGATGTGATGGACAGCACCACCAC CCAGAAATACTGGATTGACATCCAGTTGCGCTGGGGGGACTATGATTCCCACGACATTGAGCGCTACGCC CGGGCCAAGTTCCTGGACTACACCACCGACAACATGAGTATCTACCCTTCGCCCACAGGTGTACTCATCG CCATTGACCTGGCCTATAACTTGCACAGTGCCTATGGAAACTGGTTCCCAGGCAGCAAGCCTCTCATACA ACAGGCCATGGCCAAGATCATGAAGGCAAACCCTGCCCTGTATGTGTTACGTGAACGGATCCGCAAGGGG CTACAGCTCTATTCATCTGAACCCACTGAGCCTTATTTGTCTTCTCAGAACTATGGTGAGCTCTTCTCCA ACCAGATTATCTGGTTTGTGGATGACACCAACGTCTACAGAGTGACTATTCACAAGACCTTTGAAGGGAA CTTGACAACCAAGCCCATCAACGGAGCCATCTTCATCTTCAACCCACGCACAGGGCAGCTGTTCCTCAAG ATAATCCACACGTCCGTGTGGGCGGGACAGAAGCGTTTGGGGCAGTTGGCTAAGTGGAAGACAGCTGAGG AGGTGGCCGCCCTGATCCGATCTCTGCCTGTGGAGGAGCAGCCCAAGCAGATCATTGTCACCAGGAAGGG CATGCTGGACCCACTGGAGGTGCACTTACTGGACTTCCCCAATATTGTCATCAAAGGATCGGAGCTCCAA CTCCCTTTCCAGGCGTGTCTCAAGGTGGAAAAATTCGGGGATCTCATCCTTAAAGCCACTGAGCCCCAGA TGGTTCTCTTCAACCTCTATGACGACTGGCTCAAGACTATTTCATCTTACACGGCCTTCTCCCGTCTCAT CCTGATTCTGCGTGCCCTACATGTGAACAACGATCGGGCAAAAGTGATCCTGAAGCCAGACAAGACTACT ATTACAGAACCACACCACATCTGGCCCACTCTGACTGACGAAGAATGGATCAAGGTCGAGGTGCAGCTCA AGGATCTGATCTTGGCTGACTACGGCAAGAAAAACAATGTGAACGTGGCATCACTGACACAATCAGAAAT TCGAGACATCATCCTGGGTATGGAGATCTCGGCACCGTCACAGCAGCGGCAGCAGATCGCTGAGATCGAG AAGCAGACCAAGGAACAATCGCAGCTGACGGCAACACAGACTCGCACTGTCAACAAGCATGGCGATGAGA TCATCACCTCCACCACCAGCAACTATGAGACCCAGACTTTCTCATCCAAGACTGAGTGGAGGGTCAGGGC CATCTCTGCTGCCAACCTGCACCTAAGGACCAATCACATCTATGTTTCATCTGACGACATCAAGGAGACT GGCTACACCTACATCCTTCCCAAGAATGTGCTTAAGAAGTTCATCTGCATATCTGACCTTCGGGCCCAAA TTGCAGGATACCTATATGGGGTGAGCCCACCAGATAACCCCCAGGTGAAGGAGATCCGCTGCATTGTGAT GGTGCCGCAGTGGGGCACTCACCAGACCGTGCACCTGCCTGGCCAGCTGCCCCAGCATGAGTACCTCAAG GAGATGGAACCCTTAGGTTGGATCCACACTCAGCCCAATGAGTCCCCGCAGTTATCACCCCAGGATGTCA CCACCCATGCCAAGATCATGGCTGACAACCCATCTTGGGATGGCGAGAAGACCATTATCATCACATGCAG CTTCACGCCAGGCTCCTGTACACTGACGGCCTACAAGCTGACCCCCAGTGGCTACGAATGGGGCCGCCAG AACACAGACAAGGGCAACAACCCCAAGGGCTACCTGCCTTCACACTATGAGAGGGTGCAGATGCTGCTGT CGGACCGTTTCCTTGGCTTCTTCATGGTCCCTGCCCAGTCCTCGTGGAACTACAACTTCATGGGTGTTCG GCATGACCCCAACATGAAATATGAGCTACAGCTGGCGAACCCCAAAGAGTTCTACCACGAGGTGCACAGG CCCTCTCACTTCCTCAACTTTGCTCTCCTGCAGGAGGGGGAGGTTTACTCTGCGGATCGGGAGGACCTGT ATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209472 representing NM_006445
Red=Cloning site Green=Tags(s) MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKII RDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVY ISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDH QPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFE PLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAF YFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWV EVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDS HVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFW AAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEG IKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKT VCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYD VEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESR FEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDL LVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDP NNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMC GFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSM GHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQN RRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNY RTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSP TINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQ KETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKG LQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLK IIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQ LPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTT ITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIE KQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLK EMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQ NTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHR PSHFLNFALLQEGEVYSADREDLYA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006445 |
ORF Size | 7005 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_006445.3, NP_006436.3 |
RefSeq Size | 7311 |
RefSeq ORF | 7008 |
Locus ID | 10594 |
Domains | JAB_MPN |
Protein Families | Druggable Genome |
Protein Pathways | Spliceosome |
MW | 273.4 kDa |
Gene Summary | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116070 | PRPF8 (untagged)-Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8) |
USD 310.00 |
|
RG209472 | PRPF8 (GFP-tagged) - Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8) |
USD 2,200.00 |
|
RC209472L1 | Lenti ORF clone of Human PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) (PRPF8), Myc-DDK-tagged |
USD 2,199.00 |
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