KLHL13 (NM_033495) Human Tagged ORF Clone

CAT#: RC209484

KLHL13 (Myc-DDK-tagged)-Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY409529) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209484 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KLHL13 protein (Cat# TP309484). The protein was produced from HEK293T cells transfected with KLHL13 cDNA clone (Cat# RC209484) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLHL13
• Synonyms: BKLHD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209484 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATTGAAATGGAAAACGAGCTCTCCTGCTATCTGGAAATTCCCAGTTCCTGTGCTTAAAACATCCA
  GGTCAACTCCACTTTCTCCAGCATACATATCTCTCGTGGAAGAGGAAGACCAACACATGAAATTGTCCCT
  TGGAGGCAGCGAAATGGGCCTCTCATCCCATTTGCAGTCTTCCAAGGCAGGACCTACACGCATCTTTACC
  AGCAATACCCACAGTTCTGTGGTGTTACAGGGCTTTGACCAGCTTCGACTTGAAGGATTGCTTTGTGATG
  TGACCCTGATGCCAGGTGACACAGATGATGCTTTCCCTGTGCATAGAGTCATGATGGCATCTGCTAGTGA
  TTACTTCAAGGCTATGTTCACAGGTGGAATGAAAGAACAAGATTTAATGTGCATTAAACTTCATGGTGTG
  AGCAAAGTCGGTCTAAGGAAAATTATTGATTTCATTTATACTGCAAAGCTTTCTCTTAATATGGACAACC
  TTCAAGACACGCTGGAAGCTGCCAGTTTCCTACAGATTCTGCCAGTTTTGGACTTCTGTAAAGTGTTTCT
  CATATCTGGGGTCACTTTAGACAACTGTGTTGAAGTTGGACGGATTGCCAACACCTACAATCTAACCGAA
  GTGGATAAATACGTTAACAGTTTCGTCTTGAAGAATTTTCCTGCATTGCTGAGCACAGGGGAGTTCTTGA
  AACTCCCTTTTGAGCGTCTTGCCTTCGTGCTTTCCAGTAATAGCCTTAAGCACTGTACTGAACTTGAGCT
  CTTTAAGGCTACCTGTCGTTGGCTTCGCCTGGAAGAGCCTCGGATGGACTTTGCTGCAAAATTAATGAAG
  AACATACGATTTCCACTGATGACACCACAGGAGCTCATTAATTACGTGCAAACGGTGGATTTCATGAGAA
  CTGACAATACTTGTGTGAATTTGCTTTTGGAAGCCAGCAATTACCAAATGATGCCATATATGCAGCCAGT
  TATGCAGTCAGACAGGACTGCCATTAGGTCTGACACCACTCACTTGGTTACACTAGGAGGAGTGCTGAGG
  CAGCAGCTGGTTGTCAGTAAGGAATTGCGCATGTATGATGAAAAGGCCCATGAGTGGAAATCGTTAGCCC
  CCATGGATGCCCCAAGGTACCAGCATGGCATCGCCGTCATTGGAAATTTTCTCTATGTGGTTGGCGGACA
  GAGTAATTATGATACAAAAGGAAAAACGGCAGTTGATACAGTCTTCAGATTTGATCCTCGATACAATAAA
  TGGATGCAAGTTGCATCTTTAAATGAAAAGCGCACCTTCTTCCACCTAAGTGCCCTCAAAGGATATCTGT
  ATGCAGTTGGTGGGCGAAATGCAGCAGGTGAACTGCCCACAGTAGAATGTTACAATCCAAGAACAAATGA
  ATGGACCTATGTTGCCAAAATGAGTGAGCCCCACTATGGCCATGCTGGAACTGTGTATGGAGGAGTGATG
  TATATTTCAGGAGGAATTACTCATGATACTTTCCAAAAGGAGCTCATGTGCTTTGACCCTGATACTGACA
  AATGGATCCAGAAGGCGCCAATGACCACTGTCAGAGGTCTGCATTGCATGTGTACAGTGGGAGAAAGGCT
  CTATGTCATTGGTGGCAATCACTTCAGAGGAACAAGTGATTATGATGATGTCCTAAGCTGTGAATACTAT
  TCACCTATCCTTGACCAGTGGACCCCAATTGCTGCCATGTTAAGAGGGCAGAGTGATGTTGGGGTCGCTG
  TCTTCGAAAATAAAATCTATGTGGTTGGGGGGTATTCTTGGAATAATCGTTGTATGGTAGAGATAGTGCA
  GAAATATGATCCAGATAAAGATGAATGGCATAAGGTTTTTGATCTGCCAGAATCCCTTGGTGGCATTCGT
  GCTTGCACACTCACAGTTTTTCCACCAGAAGAAACCACACCATCACCTTCTAGAGAGTCCCCTCTTTCTG
  CACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209484 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLKWKTSSPAIWKFPVPVLKTSRSTPLSPAYISLVEEEDQHMKLSLGGSEMGLSSHLQSSKAGPTRIFT
  SNTHSSVVLQGFDQLRLEGLLCDVTLMPGDTDDAFPVHRVMMASASDYFKAMFTGGMKEQDLMCIKLHGV
  SKVGLRKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVTLDNCVEVGRIANTYNLTE
  VDKYVNSFVLKNFPALLSTGEFLKLPFERLAFVLSSNSLKHCTELELFKATCRWLRLEEPRMDFAAKLMK
  NIRFPLMTPQELINYVQTVDFMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDTTHLVTLGGVLR
  QQLVVSKELRMYDEKAHEWKSLAPMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRFDPRYNK
  WMQVASLNEKRTFFHLSALKGYLYAVGGRNAAGELPTVECYNPRTNEWTYVAKMSEPHYGHAGTVYGGVM
  YISGGITHDTFQKELMCFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSDYDDVLSCEYY
  SPILDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPDKDEWHKVFDLPESLGGIR
  ACTLTVFPPEETTPSPSRESPLSAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033495
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033495.2, NM_033495.3, NP_277030.2
• RefSeq Size: 3991 bp
• RefSeq ORF: 1968 bp
• Locus ID: 90293
• Domains: BTB, Kelch
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 73.9 kDa
• Gene Summary: This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]