ASB6 (NM_017873) Human Tagged ORF Clone

CAT#: RC209519

ASB6 (Myc-DDK-tagged)-Human ankyrin repeat and SOCS box containing 6 (ASB6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413495) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209519 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ASB6 protein (Cat# TP309519). The protein was produced from HEK293T cells transfected with ASB6 cDNA clone (Cat# RC209519) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASB6
• Synonyms: FLJ20548; MGC1024
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209519 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTCCTGCACGGCTTCCGGAGGATCATCTTCGAGTACCAGCCGCTGGTGGATGCGATTCTGGGCT
  CCCTGGGGATCCAGGACCCCGAGCGGCAGGAGTCTCTGGACCGGCCCAGTTATGTCGCCAGCGAGGAGAG
  CCGAATCCTTGTTCTCACTGAGCTGCTGGAGAGGAAAGCCCACTCTCCCTTTTACCAGGAAGGCGTCAGC
  AACGCCCTGCTCAAGATGGCTGAGCTGGGGCTGACGCGGGCGGCCGACGTTCTCTTGCGGCATGGGGCCA
  ATCTCAACTTTGAAGACCCAGTCACCTACTACACGGCCTTGCACATCGCCGTCCTGCGGAACCAGCCGGA
  CATGGTGGAGCTGCTGGTGCATCACGGGGCCGACGTTAATCGGAGGGACCGGATCCACGAGAGTAGCCCC
  TTGGACCTGGCCAGCGAGGAGCCTGAGCGCCTGCCCTGCCTGCAGCGCCTCCTGGACCTTGGAGCTGATG
  TCAATGCCGCTGACAAGCATGGAAAAACTGCTCTGCTCCATGCTCTGGCCAGCAGCGACGGGGTGCAGAT
  CCACAATACTGAGAACATTCGTCTCTTACTGGAAGGAGGGGCAGACGTCAAGGCCACCACCAAAGATGGG
  GACACAGTGTTCACCTGCATCATCTTCCTGCTTGGTGAGACCGTGGGAGGGGACAAAGAGGAGGCCCAGA
  TGATCAACCGCTTCTGCTTCCAAGTCACACGGCTGCTGCTGGCACACGGGGCCGACCCCAGCGAGTGCCC
  AGCCCACGAGTCCCTCACCCACATCTGCCTGAAGAGCTTTAAACTGCACTTCCCTCTCCTGCGCTTCCTC
  CTGGAGTCCGGAGCTGCCTACAACTGCTCCCTGCACGGTGCGTCCTGCTGGTCTGGCTTTCACATCATCT
  TTGAGAGGCTCTGTTCCCACCCAGGCTGCACGGAAGACGAGAGCCATGCGGACCTCCTGCGCAAAGCTGA
  GACTGTCCTGGATCTCATGGTGACCAACTCTCAGAAACTCCAGCTGCCCGAAAACTTCGATATCCACCCT
  GTGGGCAGCCTGGCGGAAAAGATCCAGGCCCTCCACTTCTCCTTGAGGCAGCTGGAGAGCTATCCCCCGC
  CCCTCAAGCACCTGTGCCGTGTGGCCATCCGGCTCTACCTTCAGCCGTGGCCTGTGGATGTGAAGGTCAA
  AGCCCTGCCTCTGCCCGACAGGCTGAAGTGGTACCTCCTTAGCGAGCACAGTGGCTCCGTGGAAGATGAC
  ATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209519 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPFLHGFRRIIFEYQPLVDAILGSLGIQDPERQESLDRPSYVASEESRILVLTELLERKAHSPFYQEGVS
  NALLKMAELGLTRAADVLLRHGANLNFEDPVTYYTALHIAVLRNQPDMVELLVHHGADVNRRDRIHESSP
  LDLASEEPERLPCLQRLLDLGADVNAADKHGKTALLHALASSDGVQIHNTENIRLLLEGGADVKATTKDG
  DTVFTCIIFLLGETVGGDKEEAQMINRFCFQVTRLLLAHGADPSECPAHESLTHICLKSFKLHFPLLRFL
  LESGAAYNCSLHGASCWSGFHIIFERLCSHPGCTEDESHADLLRKAETVLDLMVTNSQKLQLPENFDIHP
  VGSLAEKIQALHFSLRQLESYPPPLKHLCRVAIRLYLQPWPVDVKVKALPLPDRLKWYLLSEHSGSVEDD
  I
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017873
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017873.1, NM_017873.2, NM_017873.3, NP_060343.1
• RefSeq Size: 4657 bp
• RefSeq ORF: 1266 bp
• Locus ID: 140459
• Domains: SOCS, ANK
• Protein Families: Druggable Genome
• MW: 47.1 kDa
• Gene Summary: The protein encoded by this gene belongs to a family of ankyrin repeat proteins that, along with four other protein families, contain a C-terminal SOCS box motif. Growing evidence suggests that the SOCS box, similar to the F-box, acts as a bridge between specific substrate-binding domains and the more generic proteins that comprise a large family of E3 ubiquitin protein ligases. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jan 2011]