RIP3 (RIPK3) (NM_006871) Human Tagged ORF Clone

CAT#: RC209549

RIPK3 (Myc-DDK-tagged)-Human receptor-interacting serine-threonine kinase 3 (RIPK3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIPK3
• Synonyms: RIP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209549 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTGCGTCAAGTTATGGCCCAGCGGTGCCCCCGCCCCCTTGGTGTCCATCGAGGAACTGGAGAACC
  AGGAGCTCGTCGGCAAAGGCGGGTTCGGCACAGTGTTCCGGGCGCAACATAGGAAGTGGGGCTACGATGT
  GGCGGTCAAGATCGTAAACTCGAAGGCGATATCCAGGGAGGTCAAGGCCATGGCAAGTCTGGATAACGAA
  TTCGTGCTGCGCCTAGAAGGGGTTATCGAGAAGGTGAACTGGGACCAAGATCCCAAGCCGGCTCTGGTGA
  CTAAATTCATGGAGAACGGCTCCTTGTCGGGGCTGCTGCAGTCCCAGTGCCCTCGGCCCTGGCCGCTCCT
  TTGCCGCCTGCTGAAAGAAGTGGTGCTTGGGATGTTTTACCTGCACGACCAGAACCCGGTGCTCCTGCAC
  CGGGACCTCAAGCCATCCAACGTCCTGCTGGACCCAGAGCTGCACGTCAAGCTGGCAGATTTTGGCCTGT
  CCACATTTCAGGGAGGCTCACAGTCAGGGACAGGGTCCGGGGAGCCAGGGGGCACCCTGGGCTACTTGGC
  CCCAGAACTGTTTGTTAACGTAAACCGGAAGGCCTCCACAGCCAGTGACGTCTACAGCTTCGGGATCCTA
  ATGTGGGCAGTGCTTGCTGGAAGAGAAGTTGAGTTGCCAACCGAACCATCACTCGTGTACGAAGCAGTGT
  GCAACAGGCAGAACCGGCCTTCATTGGCTGAGCTGCCCCAAGCCGGGCCTGAGACTCCCGGCTTAGAAGG
  ACTGAAGGAGCTAATGCAGCTCTGCTGGAGCAGTGAGCCCAAGGACAGACCCTCCTTCCAGGAATGCCTA
  CCAAAAACTGATGAAGTCTTCCAGATGGTGGAGAACAATATGAATGCTGCTGTCTCCACGGTAAAGGATT
  TCCTGTCTCAGCTCAGGAGCAGCAATAGGAGATTTTCTATCCCAGAGTCAGGCCAAGGAGGGACAGAAAT
  GGATGGCTTTAGGAGAACCATAGAAAACCAGCACTCTCGTAATGATGTCATGGTTTCTGAGTGGCTAAAC
  AAACTGAATCTAGAGGAGCCTCCCAGCTCTGTTCCTAAAAAATGCCCGAGCCTTACCAAGAGGAGCAGGG
  CACAAGAGGAGCAGGTTCCACAAGCCTGGACAGCAGGCACATCTTCAGATTCGATGGCCCAACCTCCCCA
  GACTCCAGAGACCTCAACTTTCAGAAACCAGATGCCCAGCCCTACCTCAACTGGAACACCAAGTCCTGGA
  CCCCGAGGGAATCAGGGGGCTGAGAGACAAGGCATGAACTGGTCCTGCAGGACCCCGGAGCCAAATCCAG
  TAACAGGGCGACCGCTCGTTAACATATACAACTGCTCTGGGGTGCAAGTTGGAGACAACAACTACTTGAC
  TATGCAACAGACAACTGCCTTGCCCACATGGGGCTTGGCACCTTCGGGCAAGGGGAGGGGCTTGCAGCAC
  CCCCCACCAGTAGGTTCGCAAGAAGGCCCTAAAGATCCTGAAGCCTGGAGCAGGCCACAGGGTTGGTATA
  ATCATAGCGGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209549 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNE
  FVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLH
  RDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGIL
  MWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECL
  PKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLN
  KLNLEEPPSSVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMPSPTSTGTPSPG
  PRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQVGDNNYLTMQQTTALPTWGLAPSGKGRGLQH
  PPPVGSQEGPKDPEAWSRPQGWYNHSGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006871
• ORF Size: 1554 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006871.1, NM_006871.2, NM_006871.3, NP_006862.2
• RefSeq Size: 1940 bp
• RefSeq ORF: 1557 bp
• Locus ID: 11035
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway
• MW: 56.9 kDa
• Gene Summary: The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]