TRIM11 (NM_145214) Human Tagged ORF Clone

CAT#: RC209630

TRIM11 (Myc-DDK-tagged)-Human tripartite motif containing 11 (TRIM11)

Product Images

Western blot validation of overexpression lysate (Cat# LY403427) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209630 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TRIM11 protein (Cat# TP309630). The protein was produced from HEK293T cells transfected with TRIM11 cDNA clone (Cat# RC209630) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM11
• Synonyms: BIA1; RNF92
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209630 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCCCCGACCTGTCCACCAACCTCCAGGAGGAGGCCACCTGCGCCATCTGCCTCGACTACTTCA
  CGGATCCGGTGATGACCGACTGCGGCCACAACTTCTGCCGCGAGTGCATCCGGCGCTGCTGGGGCCAGCC
  CGAGGGCCCGTACGCGTGCCCCGAGTGCCGCGAGCTGTCCCCGCAGAGGAACCTGCGGCCCAACCGCCCG
  CTTGCTAAGATGGCCGAGATGGCGCGGCGCCTGCACCCGCCGTCGCCGGTCCCGCAGGGCGTGTGCCCCG
  CGCACCGCGAGCCACTGGCCGCCTTCTGTGGCGACGAGCTGCGCCTCCTGTGTGCGGCCTGCGAGCGCTC
  TGGGGAGCACTGGGCGCACCGCGTGCGGCCGCTGCAGGACGCGGCCGAAGACCTCAAGGCGAAGCTGGAG
  AAGTCACTGGAGCATCTCCGGAAGCAGATGCAGGATGCGTTGCTGTTCCAAGCCCAGGCGGATGAGACCT
  GCGTCTTGTGGCAGAAGATGGTGGAGAGCCAGCGGCAGAACGTGCTGGGTGAGTTCGAGCGTCTTCGCCG
  TTTGCTGGCAGAGGAGGAGCAGCAGCTGCTGCAGAGGCTGGAGGAGGAGGAGCTGGAGGTGCTGCCCCGG
  CTGCGGGAGGGCGCAGCCCACCTAGGCCAGCAGAGCGCCCACCTAGCTGAGCTCATCGCCGAGCTCGAGG
  GCCGCTGCCAGCTGCCTGCTCTGGGGCTGCTGCAGGACATCAAGGACGCCCTGCGCAGGGTCCAGGATGT
  GAAGCTGCAGCCCCCAGAAGTTGTGCCTATGGAGCTGAGGACCGTGTGCAGGGTCCCGGGACTGGTAGAG
  ACACTGCGGAGGTTTCGAGGGGACGTGACCTTGGACCCGGACACCGCCAACCCTGAGCTGATCCTGTCTG
  AAGACAGGCGGAGCGTGCAGCGGGGGGACCTACGGCAGGCCCTGCCGGACAGCCCAGAGCGCTTTGACCC
  CGGCCCCTGCGTGCTGGGCCAGGAGCGCTTCACCTCAGGCCGCCACTACTGGGAGGTGGAGGTTGGGGAC
  CGCACCAGCTGGGCCCTGGGGGTGTGCAGGGAGAACGTGAACAGGAAGGAGAAGGGCGAGCTGTCCGCGG
  GCAACGGCTTCTGGATCCTGGTCTTCCTGGGGAGCTATTACAATTCCTCGGAACGGGCCTTGGCTCCACT
  CCGGGACCCACCCAGGCGCGTGGGGATCTTTCTGGACTACGAGGCTGGACATCTCTCTTTCTACAGTGCC
  ACCGATGGGTCACTGCTATTCATCTTTCCCGAGATCCCCTTCTCGGGGACGCTGCGGCCCCTCTTCTCAC
  CCCTGTCCAGCAGCCCGACCCCGATGACTATCTGCCGGCCGAAAGGTGGGTCCGGGGACACCCTGGCTCC
  CCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209630 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPDLSTNLQEEATCAICLDYFTDPVMTDCGHNFCRECIRRCWGQPEGPYACPECRELSPQRNLRPNRP
  LAKMAEMARRLHPPSPVPQGVCPAHREPLAAFCGDELRLLCAACERSGEHWAHRVRPLQDAAEDLKAKLE
  KSLEHLRKQMQDALLFQAQADETCVLWQKMVESQRQNVLGEFERLRRLLAEEEQQLLQRLEEEELEVLPR
  LREGAAHLGQQSAHLAELIAELEGRCQLPALGLLQDIKDALRRVQDVKLQPPEVVPMELRTVCRVPGLVE
  TLRRFRGDVTLDPDTANPELILSEDRRSVQRGDLRQALPDSPERFDPGPCVLGQERFTSGRHYWEVEVGD
  RTSWALGVCRENVNRKEKGELSAGNGFWILVFLGSYYNSSERALAPLRDPPRRVGIFLDYEAGHLSFYSA
  TDGSLLFIFPEIPFSGTLRPLFSPLSSSPTPMTICRPKGGSGDTLAPQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145214
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145214.1, NM_145214.2, NP_660215.1
• RefSeq Size: 2715 bp
• RefSeq ORF: 1407 bp
• Locus ID: 81559
• Domains: zf-B_box, RING, SPRY, PRY
• Protein Families: Druggable Genome
• MW: 52.8 kDa
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nucleus and the cytoplasm. Its function has not been identified. [provided by RefSeq, Jul 2008]