GPR32 (NM_001506) Human Tagged ORF Clone

CAT#: RC209670

GPR32 (Myc-DDK-tagged)-Human G protein-coupled receptor 32 (GPR32)

Product Images

Western blot validation of overexpression lysate (Cat# LY400579) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209670 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR32
• Synonyms: RVDR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209670 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGGGTCTCGGAGGGGACCAGAGGCTGCAGTGACAGGCAACCTGGGGTCCTGACACGTGATCGCT
  CTTGTTCCAGGAAGATGAACTCTTCCGGATGCCTGTCTGAGGAGGTGGGGTCCCTCCGCCCACTGACTGT
  GGTTATCCTGTCTGCGTCCATTGTCGTCGGAGTGCTGGGCAATGGGCTGGTGCTGTGGATGACTGTCTTC
  CGTATGGCACGCACGGTCTCCACCGTCTGCTTCTTCCACCTGGCCCTTGCCGATTTCATGCTCTCACTGT
  CTCTGCCCATTGCCATGTACTATATTGTCTCCAGGCAGTGGCTCCTCGGAGAGTGGGCCTGCAAACTCTA
  CATCACCTTTGTGTTCCTCAGCTACTTTGCCAGTAACTGCCTCCTTGTCTTCATCTCTGTGGACCGTTGC
  ATCTCTGTCCTCTACCCCGTCTGGGCCCTGAACCACCGCACTGTGCAGCGGGCGAGCTGGCTGGCCTTTG
  GGGTGTGGCCCCTGGCCGCCGCCTTGTGCTCTGCGCACCTGAAATTCCGGACAACCAGAAAATGGGATGG
  CTGTACGCACTGCTACTTGGCGTTCAACTCTGACAATGAGACTGCCCAGATTTGGATTGAAGGGGTCGTG
  GAGGGACACATTATAGGGACCATTGGCCACTTCCTGCTGGGCTTCCTGGGGCCCTTAGCAATCATAGGCA
  CCTGCGCCCACCTCATCCGGGCCAAGCTCTTGCGGGAGGGCTGGGTCCATGCCAACCGGCCCAAGAGGCT
  GCTGCTGGTGCTGGTGAGCGCTTTCTTTATCTTCTGGTCCCCGTTTAACGTGGTGCTGTTGGTCCATCTG
  TGGCGACGGGTGATGCTCAAGGAAATCTACCACCCCCGGATGCTGCTCATCCTCCAGGCTAGCTTTGCCT
  TGGGCTGTGTCAACAGCAGCCTCAACCCCTTCCTCTACGTCTTCGTTGGCAGAGATTTCCAAGAAAAGTT
  TTTCCAGTCTTTGACTTCTGCCCTGGCGAGGGCGTTTGGAGAGGAGGAGTTTCTGTCATCCTGTCCCCGT
  GGCAACGCCCCCCGGGAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209670 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNGVSEGTRGCSDRQPGVLTRDRSCSRKMNSSGCLSEEVGSLRPLTVVILSASIVVGVLGNGLVLWMTVF
  RMARTVSTVCFFHLALADFMLSLSLPIAMYYIVSRQWLLGEWACKLYITFVFLSYFASNCLLVFISVDRC
  ISVLYPVWALNHRTVQRASWLAFGVWPLAAALCSAHLKFRTTRKWDGCTHCYLAFNSDNETAQIWIEGVV
  EGHIIGTIGHFLLGFLGPLAIIGTCAHLIRAKLLREGWVHANRPKRLLLVLVSAFFIFWSPFNVVLLVHL
  WRRVMLKEIYHPRMLLILQASFALGCVNSSLNPFLYVFVGRDFQEKFFQSLTSALARAFGEEEFLSSCPR
  GNAPRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001506
• ORF Size: 1070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001506.1, NM_001506.2, NP_001497.1
• RefSeq Size: 1269 bp
• RefSeq ORF: 1071 bp
• Locus ID: 2854
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 40.1 kDa
• Gene Summary: This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. The encoded protein binds to resolvin D1 and lipoxin A4 and has been linked to pulmonary inflammation. A related pseudogene has been identified on chromosome 19. [provided by RefSeq, Nov 2012]