TPK1 (NM_022445) Human Tagged ORF Clone

CAT#: RC209721L4

Lenti ORF clone of Human thiamin pyrophosphokinase 1 (TPK1), transcript variant 1, mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: TPK1
• Synonyms: HTPK1; PP20; THMD5
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC209721).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_022445
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022445.3, NP_071890.2
• RefSeq Size: 2449
• RefSeq ORF: 732
• Locus ID: 27010
• Domains: TPK_catalytic, TPK_B1_binding
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Thiamine metabolism
• MW: 27.2 kDa
• Gene Summary: The protein encoded by this gene functions as a homodimer and catalyzes the conversion of thiamine to thiamine pyrophosphate, a cofactor for some enzymes of the glycolytic and energy production pathways. Defects in this gene are a cause of thiamine metabolism dysfunction syndrome-5. [provided by RefSeq, Apr 2017]