C8orf76 (NM_032847) Human Tagged ORF Clone

CAT#: RC209778

C8orf76 (Myc-DDK-tagged)-Human chromosome 8 open reading frame 76 (C8orf76)

Product Images

Western blot validation of overexpression lysate (Cat# LY409883) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209778 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C8orf76
• Synonyms: FLJ14825; MGC9784
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209778 representing NM_032847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCCGGGTGCTGGTTGTTCGGCGGCGAGTTCGAGGACTCGGTGTTCGAGGAGAGGCCGGAGCGGC
  GGTCAGGACCGCCCGCGTCCTACTGCGCCAAGCTCTGCGAGCCGCAGTGGTTTTATGAAGAAACAGAAAG
  CAGTGATGATGTTGAAGTGCTGACTCTCAAGAAATTCAAAGGAGACCTGGCCTACAGACGACAAGAGTAT
  CAGAAAGCACTGCAGGAGTATTCCAGTATCTCTGAAAAATTGTCATCAACCAATTTTGCCATGAAAAGGG
  ATGTCCAGGAAGGTCAGGCTCGGTGTCTGGCTCACCTGGGTAGGCATATGGAGGCGCTGGAGATTGCTGC
  AAACTTGGAAAATAAAGCAACCAACACAGACCATTTAACCACGGTACTCTACCTCCAGCTTGCTATTTGT
  TCAAGTTTGCAGAACTTGGAGAAAACAATTTTCTGCCTGCAGAAACTGATTTCTTTGCATCCTTTTAATC
  CTTGGAACTGGGGCAAATTGGCAGAGGCTTACCTGAATCTGGGGCCAGCTCTTTCAGCAGCACTTGCGTC
  ATCTCAGAAACAGCACAGTTTCACCTCAAGTGACAAAACTATCAAATCCTTCTTTCCACACTCAGGAAAA
  GACTGTCTTTTGTGTTTTCCTGAAACCTTGCCTGAGAGCTCTTTATTTTCTGTGGAAGCGAATAGCAGTA
  ATAGCCAGAAAAATGAGAAAGCTCTGACAAATATCCAAAACTGTATGGCAGAAAAGAGAGAAACAGTGTT
  GATAGAGACTCAGCTGAAAGCATGTGCCTCTTTTATACGAACCAGGCTTCTGCTTCAGTTTACCCAACCT
  CAGCAAACATCGTTTGCTTTGGAGAGGAACTTAAGGACTCAGCAGGAAATTGAAGATAAAATGAAAGGGT
  TCAGCTTCAAAGAAGACACTTTGCTGTTGATAGCTGAGGTTATGGGAGAAGATATCCCAGAAAAAATAAA
  AGATGAAGTTCACCCAGAGGTGAAGTGTGTTGGCTCCGTAGCCCTGACTGCCTTGGTGACTGTATCCTCA
  GAAGAATTTGAAGACAAGTGGTTCAGAAAGATCAAAGACCATTTCTGTCCATTTGAAAATCAGTTCCATA
  CAGAGATACAAATCTTGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209778 representing NM_032847
  Red=Cloning site Green=Tags(s)
  
  MDSGCWLFGGEFEDSVFEERPERRSGPPASYCAKLCEPQWFYEETESSDDVEVLTLKKFKGDLAYRRQEY
  QKALQEYSSISEKLSSTNFAMKRDVQEGQARCLAHLGRHMEALEIAANLENKATNTDHLTTVLYLQLAIC
  SSLQNLEKTIFCLQKLISLHPFNPWNWGKLAEAYLNLGPALSAALASSQKQHSFTSSDKTIKSFFPHSGK
  DCLLCFPETLPESSLFSVEANSSNSQKNEKALTNIQNCMAEKRETVLIETQLKACASFIRTRLLLQFTQP
  QQTSFALERNLRTQQEIEDKMKGFSFKEDTLLLIAEVMGEDIPEKIKDEVHPEVKCVGSVALTALVTVSS
  EEFEDKWFRKIKDHFCPFENQFHTEIQILA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032847
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032847.1, NM_032847.2, NP_116236.1
• RefSeq Size: 1286
• RefSeq ORF: 1143
• Locus ID: 84933
• MW: 43.1 kDa