PUS7L (NM_031292) Human Tagged ORF Clone

CAT#: RC209780

PUS7L (Myc-DDK-tagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae)-like (PUS7L), transcript variant 3


  "NM_031292" in other vectors (4)

Reconstitution Protocol

USD 420.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PUS7L
Synonyms DKFZp434G1415; DKFZp781B2386; FLJ34711; FLJ40012
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209780 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAAGATACAGATTATAGAATCAGGTTTAGTTCTTTGTGTTTCTTTAATGATCACGTTGGATTTC
ATGGCACTATAAAAAGCTCACCAAGTGACTTTATTGTTATTGAAATTGATGAACAGGGACAGTTAGTTAA
TAAGACCATCGATGAGCCTATTTTCAAGATTAGTGAAATACAACTTGAGCCAAATAATTTTCCCAAAAAA
CCAAAACTAGATCTTCAAAATCTGTCCTTAGAAGATGGAAGAAACCAAGAAGTTCATACTTTGATTAAGT
ACACTGATGGTGACCAAAATCATCAGTCTGGTTCAGAAAAGGAAGATACTATCGTTGATGGAACTTCCAA
ATGTGAAGAAAAAGCTGATGTTTTAAGCTCCTTTTTGGATGAAAAAACTCATGAGTTACTGAATAATTTT
GCCTGTGATGTAAGAGAGAAGTGGCTTTCTAAAACAGAGCTAATTGGACTACCTCCTGAATTCTCAATAG
GCAGAATCGTTGACAAAAACCAGAGGGCTAGTTTACACAGTGCCATTAGGCAGAAATTTCCATTTTTAGT
AACTGTAGGAAAAAACAGTGAAATTGTTGTAAAACCAAATCTTGAATATAAAGAACTTTGTCATTTGGTA
TCTGAAGAGGAAGCATTTGACTTTTTTAAATATTTGGATGCAAAGAAAGAAAATTCCAAATTTACCTTTA
AACCTGATACAAACAAAGACCACAGAAAAGCTGTCCACCATTTTGTCAACAAAAAGTTTGGAAACCTTGT
GGAAACCAAATCTTTTTCTAAAATGAATTGCAGTGCTGGTAATCCGAATGTGGTGGTAACAGTAAGATTT
CGGGAAAAAGCACACAAACGTGGGAAAAGGCCTCTTTCTGAATGCCAAGAAGGAAAAGTTATATATACAG
CTTTTACCCTACGAAAGGAAAACCTGGAAATGTTTGAAGCGATTGGTTTTTTAGCTATCAAACTTGGTGT
TATTCCTTCGGATTTTAGTTATGCAGGCCTTAAAGACAAGAAAGCCATCACCTATCAAGCAATGGTTGTT
AGAAAAGTGACTCCAGAGAGGTTGAAAAATATTGAAAAAGAAATTGAAAAGAAAAGAATGAATGTCTTTA
ATATTCGGTCTGTAGATGATTCCCTGAGACTTGGTCAGCTCAAAGGAAATCACTTTGATATTGTCATTAG
AAATTTAAAAAAACAAATAAATGATTCTGCAAACCTGAGGGAGAGAATTATGGAAGCAATAGAAAATGTT
AAGAAAAAAGGCTTTGTGAATTACTATGGACCACAGAGATTTGGGAAGGGAAGGAAAGTTCACACAGACC
AAATTGGACTAGCTTTGCTGAAGAATGAAATGATGAAAGCCATAAAATTGTTTCTTACACCAGAAGACTT
GGATGATCCTGTAAATAGAGCAAAGAAGTATTTTCTTCAAACTGAGGATGCTAAAGGCACACTTTCATTG
ATGCCTGAATTCAAAGTGCGTGAGAGAGCATTGTTGGAGGCATTGCACCGCTTTGGCATGACCGAGGAAG
GTTGTATCCAGGCATGGTTCTCTTTACCCCATTCCATGCGCATATTCTATGTTCACGCATATACCAGCAA
AATTTGGAATGAGGCAGTATCTTACAGACTTGAAACCTATGGAGCAAGAGTAGTGCAGGGTGATTTGGTC
TGTTTGGATGAAGACATTGATGACGAGAATTTCCAAAATAGTAAAATTCACCTGGTAACTGAAGAGGAGG
GATCAGCTAATATGTATGCAATACATCAGGTGGTTCTTCCAGTACTTGGATACAATATTCAGTACCCGAA
GAACAAAGTAGGGCAGTGGTACCATGACATACTTAGCAGAGATGGACTACAGACATGTAGGTTTAAAGTA
CCTACTCTGAAACTGAATATACCAGGTTGCTATAGACAGATTTTGAAACATCCCTGTAATCTCTCATACC
AACTAATGGAAGATCATGACATTGATGTCAAAACGAAAGGTTCCCACATTGATGAAACAGCTTTGTCTCT
TTTGATCTCTTTTGATCTTGATGCTTCATGCTATGCTACCGTTTGTCTGAAGGAAATAATGAAGCATGAC
GTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209780 protein sequence
Red=Cloning site Green=Tags(s)

MEEDTDYRIRFSSLCFFNDHVGFHGTIKSSPSDFIVIEIDEQGQLVNKTIDEPIFKISEIQLEPNNFPKK
PKLDLQNLSLEDGRNQEVHTLIKYTDGDQNHQSGSEKEDTIVDGTSKCEEKADVLSSFLDEKTHELLNNF
ACDVREKWLSKTELIGLPPEFSIGRIVDKNQRASLHSAIRQKFPFLVTVGKNSEIVVKPNLEYKELCHLV
SEEEAFDFFKYLDAKKENSKFTFKPDTNKDHRKAVHHFVNKKFGNLVETKSFSKMNCSAGNPNVVVTVRF
REKAHKRGKRPLSECQEGKVIYTAFTLRKENLEMFEAIGFLAIKLGVIPSDFSYAGLKDKKAITYQAMVV
RKVTPERLKNIEKEIEKKRMNVFNIRSVDDSLRLGQLKGNHFDIVIRNLKKQINDSANLRERIMEAIENV
KKKGFVNYYGPQRFGKGRKVHTDQIGLALLKNEMMKAIKLFLTPEDLDDPVNRAKKYFLQTEDAKGTLSL
MPEFKVRERALLEALHRFGMTEEGCIQAWFSLPHSMRIFYVHAYTSKIWNEAVSYRLETYGARVVQGDLV
CLDEDIDDENFQNSKIHLVTEEEGSANMYAIHQVVLPVLGYNIQYPKNKVGQWYHDILSRDGLQTCRFKV
PTLKLNIPGCYRQILKHPCNLSYQLMEDHDIDVKTKGSHIDETALSLLISFDLDASCYATVCLKEIMKHD
V

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031292
ORF Size 2103 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031292.1, NM_031292.2, NM_031292.3, NM_031292.4, NP_112582.2
RefSeq Size 3985 bp
RefSeq ORF 2106 bp
Locus ID 83448
Domains UPF0024
MW 80.7 kDa

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