CD62P (SELP) (NM_003005) Human Tagged ORF Clone

CAT#: RC209822

SELP (Myc-DDK-tagged)-Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP)

Product Images

Western blot validation of overexpression lysate (Cat# LY418960) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209822 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SELP protein (Cat# TP309822). The protein was produced from HEK293T cells transfected with SELP cDNA clone (Cat# RC209822) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SELP
• Synonyms: CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209822 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACTGCCAAATAGCCATCTTGTACCAGAGATTCCAGAGAGTGGTCTTTGGAATTTCCCAACTCC
  TTTGCTTCAGTGCCCTGATCTCTGAACTAACAAACCAGAAAGAAGTGGCAGCATGGACTTATCATTACAG
  CACAAAAGCATACTCATGGAATATTTCCCGTAAATACTGCCAGAATCGCTACACAGACTTAGTGGCCATC
  CAGAATAAAAATGAAATTGATTACCTCAATAAGGTCCTACCCTACTACAGCTCCTACTACTGGATTGGGA
  TCCGAAAGAACAATAAGACATGGACATGGGTGGGAACCAAAAAGGCTCTCACCAACGAGGCTGAGAACTG
  GGCTGATAATGAACCTAACAACAAAAGGAACAACGAGGACTGCGTGGAGATATACATCAAGAGTCCGTCA
  GCCCCTGGCAAGTGGAATGATGAGCACTGCTTGAAGAAAAAGCACGCATTGTGTTACACAGCCTCCTGCC
  AGGACATGTCCTGCAGCAAACAAGGAGAGTGCCTCGAGACCATCGGGAACTACACCTGCTCCTGTTACCC
  TGGATTCTATGGGCCAGAATGTGAATACGTGAGAGAGTGTGGAGAACTTGAGCTCCCTCAACACGTGCTC
  ATGAACTGCAGCCACCCTCTGGGAAACTTCTCTTTTAACTCGCAGTGCAGCTTCCACTGCACTGACGGGT
  ACCAAGTAAATGGGCCCAGCAAGCTGGAATGCTTGGCTTCTGGAATCTGGACAAATAAGCCTCCACAGTG
  TTTAGCTGCCCAGTGCCCACCCCTGAAGATTCCTGAACGAGGAAACATGACCTGCCTTCATTCTGCAAAA
  GCATTCCAGCATCAGTCTAGCTGCAGCTTCAGTTGTGAAGAGGGATTTGCATTAGTTGGACCGGAAGTGG
  TGCAATGCACAGCCTCGGGGGTATGGACAGCCCCAGCCCCAGTGTGTAAAGCTGTGCAGTGTCAGCACCT
  GGAAGCCCCCAGTGAAGGAACCATGGACTGTGTTCATCCGCTCACTGCTTTTGCCTATGGCTCCAGCTGT
  AAATTTGAGTGCCAGCCCGGCTACAGAGTGAGGGGCTTGGACATGCTCCGCTGCATTGACTCTGGACACT
  GGTCTGCACCCTTGCCAACCTGTGAGGCTATTTCGTGTGAGCCGCTGGAGAGTCCTGTCCACGGAAGCAT
  GGATTGCTCTCCATCCTTGAGAGCGTTTCAGTATGACACCAACTGTAGCTTCCGCTGTGCTGAAGGTTTC
  ATGCTGAGAGGAGCCGATATAGTTCGGTGTGATAACTTGGGACAGTGGACAGCACCAGCCCCAGTCTGTC
  AAGCTTTGCAGTGCCAGGATCTCCCAGTTCCAAATGAGGCCCGGGTGAACTGCTCCCACCCCTTCGGTGC
  CTTTAGGTACCAGTCAGTCTGCAGCTTCACCTGCAATGAAGGCTTGCTCCTGGTGGGAGCAAGTGTGCTA
  CAGTGCTTGGCTACTGGAAACTGGAATTCTGTTCCTCCAGAATGCCAAGCCATTCCCTGCACACCTTTGC
  TAAGCCCTCAGAATGGAACAATGACCTGTGTTCGACCTCTTGGAAGTTCCAGTTATAAATCCACATGTCA
  ATTCATCTGTGACGAGGGATATTCTTTGTCTGGACCAGAAAGATTGGATTGTACTCGATCGGGACGCTGG
  ACAGACTCCCCACCAATGTGTGAAGCCATCAAGTGCCCAGAACTCTTTGCCCCAGAGCAGGGCAGCCTGG
  ATTGTTCTGACACTCGTGGAGAATTCAATGTTGGCTCCACCTGCCATTTCTCTTGTAACAACGGCTTTAA
  GCTGGAGGGGCCCAATAATGTGGAATGCACAACTTCTGGAAGATGGTCAGCTACTCCACCAACCTGCAAA
  GGCATAGCATCACTTCCTACTCCAGGGGTGCAATGTCCAGCCCTCACCACTCCTGGGCAGGGAACCATGT
  ACTGTAGGCATCATCCGGGAACCTTTGGTTTTAATACCACTTGTTACTTTGGCTGCAACGCTGGATTCAC
  ACTCATAGGAGACAGCACTCTCAGCTGCAGACCTTCAGGACAATGGACAGCAGTAACTCCAGCATGCAGA
  GCTGTGAAATGCTCAGAACTACATGTTAATAAGCCAATAGCGATGAACTGCTCCAACCTCTGGGGAAACT
  TCAGTTATGGATCAATCTGCTCTTTCCATTGTCTAGAGGGCCAGTTACTTAATGGCTCTGCACAAACAGC
  ATGCCAAGAGAATGGCCACTGGTCAACTACCGTGCCAACCTGCCAAGGACCATTGACTATCCAGGAAGCC
  CTGACTTACTTTGGTGGAGCGGTGGCTTCTACGATAGGTTTGATAATGGGTGGGACGCTCCTGGCTTTGC
  TAAGAAAGCGTTTCAGACAAAAAGATGATGGGAAATGCCCCTTGAATCCTCACAGCCACCTAGGAACATA
  TGGAGTTTTTACAAACGCTGCATTTGACCCGAGTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209822 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANCQIAILYQRFQRVVFGISQLLCFSALISELTNQKEVAAWTYHYSTKAYSWNISRKYCQNRYTDLVAI
  QNKNEIDYLNKVLPYYSSYYWIGIRKNNKTWTWVGTKKALTNEAENWADNEPNNKRNNEDCVEIYIKSPS
  APGKWNDEHCLKKKHALCYTASCQDMSCSKQGECLETIGNYTCSCYPGFYGPECEYVRECGELELPQHVL
  MNCSHPLGNFSFNSQCSFHCTDGYQVNGPSKLECLASGIWTNKPPQCLAAQCPPLKIPERGNMTCLHSAK
  AFQHQSSCSFSCEEGFALVGPEVVQCTASGVWTAPAPVCKAVQCQHLEAPSEGTMDCVHPLTAFAYGSSC
  KFECQPGYRVRGLDMLRCIDSGHWSAPLPTCEAISCEPLESPVHGSMDCSPSLRAFQYDTNCSFRCAEGF
  MLRGADIVRCDNLGQWTAPAPVCQALQCQDLPVPNEARVNCSHPFGAFRYQSVCSFTCNEGLLLVGASVL
  QCLATGNWNSVPPECQAIPCTPLLSPQNGTMTCVRPLGSSSYKSTCQFICDEGYSLSGPERLDCTRSGRW
  TDSPPMCEAIKCPELFAPEQGSLDCSDTRGEFNVGSTCHFSCNNGFKLEGPNNVECTTSGRWSATPPTCK
  GIASLPTPGVQCPALTTPGQGTMYCRHHPGTFGFNTTCYFGCNAGFTLIGDSTLSCRPSGQWTAVTPACR
  AVKCSELHVNKPIAMNCSNLWGNFSYGSICSFHCLEGQLLNGSAQTACQENGHWSTTVPTCQGPLTIQEA
  LTYFGGAVASTIGLIMGGTLLALLRKRFRQKDDGKCPLNPHSHLGTYGVFTNAAFDPSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003005
• ORF Size: 591 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003005.1, NM_003005.2, NM_003005.3, NP_002996.1
• RefSeq Size: 3185 bp
• RefSeq ORF: 2493 bp
• Locus ID: 6403
• Cytogenetics: 1q24.2
• Domains: CCP, CLECT, EGF
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 90.8 kDa
• Gene Summary: 'This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]'