GPR126 (ADGRG6) (NM_001032395) Human Tagged ORF Clone

CAT#: RC209872

GPR126 (Myc-DDK-tagged)-Human G protein-coupled receptor 126 (GPR126), transcript variant b2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADGRG6
• Synonyms: APG1; DREG; GPR126; LCCS9; PR126; PS1TP2; STQTL1; VIGR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209872 representing NM_001032395
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTTTCGCTCAGATCGAATGTGGAGCTGCCATTGGAAATGGAAGCCCAGTCCTCTCCTGTTCTTAT
  TTGCTTTATATATCATGTGTGTTCCTCACTCAGTGTGGGGATGTGCCAACTGCCGAGTGGTTTTGTCCAA
  CCCTTCTGGGACCTTTACTTCTCCATGCTACCCTAACGACTACCCAAACAGCCAGGCTTGCATGTGGACG
  CTCCGAGCCCCCACCGGTTATATCATTCAGATAACATTTAACGACTTTGACATTGAAGAAGCTCCCAATT
  GCATTTATGACTCATTATCCCTTGATAATGGAGAGAGCCAGACTAAATTTTGTGGAGCAACTGCCAAAGG
  CCTATCATTTAACTCAAGTGCGAATGAGATGCATGTGTCCTTTTCAAGTGACTTTAGCATCCAGAAGAAA
  GGTTTCAATGCCAGCTACATCAGAGTTGCCGTGTCCTTAAGGAATCAAAAGGTCATTTTACCCCAGACAT
  CAGATGCTTACCAGGTATCTGTTGCAAAAAGCATCTCTATTCCAGAGCTCAGTGCTTTCACACTCTGCTT
  TGAAGCAACCAAAGTTGGCCATGAAGACAGTGATTGGACAGCTTTCTCCTACTCAAATGCATCCTTCACA
  CAATTGCTCAGTTTTGGAAAGGCCAAGAGTGGCTACTTTCTATCCATTTCTGATTCACAATGTTTGTTGA
  ATAATGCATTACCTGTCAAAGAAAAAGAAGACATTTTTGCAGAAAGCTTTGAACAGCTCTGCCTTGTTTG
  GAATAATTCTTTGGGCTCTATTGGTGTAAATTTCAAAAGAAACTATGAAACAGTTCCATGTGATTCTACC
  ATTAGTAAAGTTATTCCTGGGAATGGGAAATTGTTGTTGGGCTCCAATCAAAATGAAATTGTCTCTCTAA
  AAGGGGACATTTATAACTTTCGACTTTGGAATTTTACCATGAATGCCAAAATCCTCTCCAACCTCAGCTG
  TAATGTGAAAGGGAATGTAGTCGACTGGCAAAATGACTTCTGGAATATCCCAAACCTAGCTCTGAAAGCT
  GAAAGCAACCTAAGCTGTGGTTCCTACCTGATCCCGCTCCCAGCAGCAGAACTGGCCAGCTGTGCAGACC
  TGGGGACCCTCTGTCAAGATGGAATTATCTATAGAATATCCGTAGTGATTCAGAACATCCTTCGTCACCC
  TGAGGTAAAAGTACAGAGCAAGGTGGCAGAATGGCTCAATTCAACCTTCCAAAATTGGAACTACACGGTT
  TATGTCGTTAATATCAGTTTTCACCTGAGTGCTGGAGAGGACAAGATTAAAGTCAAGAGAAGCCTTGAGG
  ATGAGCCAAGGTTGGTGCTTTGGGCCCTTCTAGTTTACAATGCTACCAACAATACTAATTTAGAAGGAAA
  AATCATTCAGCAGAAGCTCCTAAAAAATAATGAGTCCTTGGATGAAGGCTTGAGGCTACATACAGTGAAT
  GTGAGACAACTGGGTCATTGTCTTGCCATGGAGGAACCCAAAGGCTACTACTGGCCATCTATCCAACCTT
  CTGAATACGTTCTTCCTTGTCCAGACAAGCCTGGCTTTTCTGCTTCTCGGATATGTTTTTACAATGCTAC
  CAACCCATTGGTAACCTACTGGGGACCTGTTGATATCTCCAACTGTTTAAAAGAAGCAAATGAAGTTGCT
  AACCAGATTTTAAATTTAACTGCTGATGGGCAGAACTTAACCTCAGCCAATATTACCAACATTGTGGAAC
  AGGTCAAAAGAATTGTGAATAAAGAAGAAAACATTGATATAACACTTGGCTCAACTCTAATGAATATATT
  TTCTAATATCTTAAGCAGTTCAGACAGTGACTTGCTTGAGTCATCTTCTGAAGCTTTAAAAACAATTGAT
  GAATTGGCCTTCAAGATAGACCTAAATAGCACATCACATGTGAATATTACAACTCGGAACTTGGCTCTCA
  GCGTATCATCCCTGTTACCAGGGACAAATGCAATTTCAAATTTTAGCATTGGTCTTCCAAGCAATAATGA
  ATCGTATTTCCAGATGGATTTTGAGAGTGGACAAGTGGATCCACTGGCATCTGTAATTTTGCCTCCAAAC
  TTACTTGAGAATTTAAGTCCAGAAGATTCTGTATTAGTTAGAAGAGCACAGTTTACTTTCTTCAACAAAA
  CTGGACTTTTCCAGGATGTAGGACCCCAAAGAAAAACTTTAGTGAGTTATGTGATGGCGTGCAGTATTGG
  AAACATTACTATCCAGAATCTGAAGGATCCTGTTCAAATAAAAATCAAACATACAAGAACTCAGGAAGTG
  CATCATCCCATCTGTGCCTTCTGGGATCTGAACAAAAACAAAAGTTTTGGAGGATGGAACACGTCAGGAT
  GTGTTGCACACAGAGATTCAGATGCAAGTGAGACAGTCTGCCTGTGTAACCACTTCACACACTTTGGAGT
  TCTGATGGACCTTCCAAGAAGTGCCTCACAGTTAGATGCAAGAAACACTAAAGTCCTCACTTTCATCAGC
  TATATTGGGTGTGGAATATCTGCTATTTTTTCAGCAGCAACTCTCCTGACATATGTTGCTTTTGAGAAAT
  TGCGAAGGGATTATCCCTCCAAAATCTTGATGAACCTGAGCACAGCCCTGCTGTTCCTGAATCTCCTCTT
  CCTCCTAGATGGCTGGATCACCTCCTTCAATGTGGATGGACTTTGCATTGCTGTTGCAGTCCTGTTGCAT
  TTCTTCCTTCTGGCAACCTTTACCTGGATGGGGCTAGAAGCAATTCACATGTACATTGCTCTAGTTAAAG
  TATTTAACACTTACATTCGCCGATACATTCTAAAATTCTGCATCATTGGCTGGGGTTTGCCTGCCTTAGT
  GGTGTCAGTTGTTCTAGCGAGCAGAAACAACAATGAAGTCTATGGAAAAGAAAGTTATGGGAAAGAAAAA
  GGTGATGAATTCTGTTGGATTCAAGATCCAGTCATATTTTATGTGACCTGTGCTGGGTATTTTGGAGTCA
  TGTTTTTTCTGAACATTGCCATGTTCATTGTGGTAATGGTGCAGATCTGTGGGAGGAATGGCAAGAGAAG
  CAACCGGACCCTGAGAGAAGAAGTGTTAAGGAACCTGCGCAGTGTGGTTAGCTTGACCTTTCTGTTGGGC
  ATGACATGGGGTTTTGCATTCTTTGCCTGGGGACCCTTAAATATCCCCTTCATGTACCTCTTCTCCATCT
  TCAATTCATTACAAGGCTTATTTATATTCATCTTCCACTGTGCTATGAAGGAGAATGTTCAGAAACAGTG
  GCGGCGGCATCTCTGCTGTGGTAGATTTCGGTTAGCAGATAACTCAGATTGGAGTAAGACAGCTACCAAT
  ATCATCAAGAAAAGTTCTGATAATCTAGGAAAATCTTTGTCTTCAAGCTCCATTGGTTCCAACTCAACCT
  ATCTTACATCCAAATCTAAATCCAGCTCTACCACCTATTTCAAAAGGAATAGCCACACAGACAGTGCTTC
  CATGGACAAGTCCTTGTCAAAACTGGCCCATGCTGATGGAGATCAAACATCAATCATCCCTGTCCATCAG
  GTCATTGATAAGGTCAAGGGTTATTGCAATGCTCATTCAGACAACTTCTATAAAAATATTATCATGTCAG
  ACACCTTCAGCCACAGCACAAAGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209872 representing NM_001032395
  Red=Cloning site Green=Tags(s)
  
  MMFRSDRMWSCHWKWKPSPLLFLFALYIMCVPHSVWGCANCRVVLSNPSGTFTSPCYPNDYPNSQACMWT
  LRAPTGYIIQITFNDFDIEEAPNCIYDSLSLDNGESQTKFCGATAKGLSFNSSANEMHVSFSSDFSIQKK
  GFNASYIRVAVSLRNQKVILPQTSDAYQVSVAKSISIPELSAFTLCFEATKVGHEDSDWTAFSYSNASFT
  QLLSFGKAKSGYFLSISDSQCLLNNALPVKEKEDIFAESFEQLCLVWNNSLGSIGVNFKRNYETVPCDST
  ISKVIPGNGKLLLGSNQNEIVSLKGDIYNFRLWNFTMNAKILSNLSCNVKGNVVDWQNDFWNIPNLALKA
  ESNLSCGSYLIPLPAAELASCADLGTLCQDGIIYRISVVIQNILRHPEVKVQSKVAEWLNSTFQNWNYTV
  YVVNISFHLSAGEDKIKVKRSLEDEPRLVLWALLVYNATNNTNLEGKIIQQKLLKNNESLDEGLRLHTVN
  VRQLGHCLAMEEPKGYYWPSIQPSEYVLPCPDKPGFSASRICFYNATNPLVTYWGPVDISNCLKEANEVA
  NQILNLTADGQNLTSANITNIVEQVKRIVNKEENIDITLGSTLMNIFSNILSSSDSDLLESSSEALKTID
  ELAFKIDLNSTSHVNITTRNLALSVSSLLPGTNAISNFSIGLPSNNESYFQMDFESGQVDPLASVILPPN
  LLENLSPEDSVLVRRAQFTFFNKTGLFQDVGPQRKTLVSYVMACSIGNITIQNLKDPVQIKIKHTRTQEV
  HHPICAFWDLNKNKSFGGWNTSGCVAHRDSDASETVCLCNHFTHFGVLMDLPRSASQLDARNTKVLTFIS
  YIGCGISAIFSAATLLTYVAFEKLRRDYPSKILMNLSTALLFLNLLFLLDGWITSFNVDGLCIAVAVLLH
  FFLLATFTWMGLEAIHMYIALVKVFNTYIRRYILKFCIIGWGLPALVVSVVLASRNNNEVYGKESYGKEK
  GDEFCWIQDPVIFYVTCAGYFGVMFFLNIAMFIVVMVQICGRNGKRSNRTLREEVLRNLRSVVSLTFLLG
  MTWGFAFFAWGPLNIPFMYLFSIFNSLQGLFIFIFHCAMKENVQKQWRRHLCCGRFRLADNSDWSKTATN
  IIKKSSDNLGKSLSSSSIGSNSTYLTSKSKSSSTTYFKRNSHTDSASMDKSLSKLAHADGDQTSIIPVHQ
  VIDKVKGYCNAHSDNFYKNIIMSDTFSHSTKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032395
• ORF Size: 3666 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032395.1, NM_001032395.2, NP_001027567.1
• RefSeq Size: 6818 bp
• RefSeq ORF: 3669 bp
• Locus ID: 57211
• Cytogenetics: 6q24.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 136.91 kDa
• Gene Summary: This gene, which is upregulated in human umbilical vein endothelial cells, encodes a G protein-coupled receptor. Variations in this gene can affect a person's stature. Multiple transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Mar 2009]