SLC12A6 (NM_001042495) Human Tagged ORF Clone

CAT#: RC209880

SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY420941) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214078 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC12A6
• Synonyms: ACCPN; KCC3; KCC3A; KCC3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209880 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGATGTCTGGGGCCACCACTTCGCTGGCAACTGTTGCACTGGATCCACCCAGTGACCGGACTT
  CTCACCCCCAGGATGTCATCGAGGACCTGAGTCAGAACTCCATCACAGGGGAACACAGCCAACTGTTAGA
  CGACGGACATAAGAAAGCTCGAAATGCTTATCTCAATAATTCCAATTATGAAGAAGGAGATGAATATTTT
  GATAAAAATTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCGAAGGTGTCTTCCCTCCTCAACCGCA
  TGGCCAATTACACTAATCTGACTCAAGGAGCAAAGGAACATGAAGAGGCAGAAAACATCACTGAAGGGAA
  AAAGAAGCCCACCAAGACCCCCCAAATGGGTACCTTCATGGGTGTCTACCCCCCATGTCTACAAAATATT
  TTTGGAGTGATCCTTTTTTTACGCCTTACATGGGTGGTGGGCACAGCTGGAGTTCTTCAGGCTTTTGCAA
  TTGTCCTTATCTGCTGCTGCTGTACAATGTTGACTGCTATCTCCATGAGTGCCATTGCCACTAATGGAGT
  GGTGCCAGCTGGGGGCTCATACTTTATGATTTCCCGGGCACTGGGCCCAGAGTTTGGTGGGGCTGTTGGC
  CTCTGCTTTTATCTTGGTACCACATTTGCAGCAGCCATGTACATCCTTGGTGCCATTGAAATCTTTCTGG
  TCTATATCGTCCCCCGAGCTGCCATCTTTCACAGTGATGACGCACTCAAGGAATCAGCAGCCATGCTAAA
  TAACATGCGTGTCTACGGCACAGCTTTCTTGGTCCTTATGGTATTAGTGGTATTTATCGGCGTACGCTAT
  GTGAACAAGTTTGCCTCACTTTTCCTGGCCTGTGTCATTGTGTCCATCTTGGCCATCTATGCTGGAGCCA
  TCAAGTCTTCTTTTGCTCCTCCACACTTCCCGGTCTGCATGCTGGGTAACCGCACCCTTTCATCAAGACA
  CATTGACGTTTGCTCTAAGACCAAGGAAATTAACAACATGACAGTCCCATCAAAGTTATGGGGATTCTTC
  TGTAACTCGAGTCAATTTTTCAATGCCACCTGTGATGAATACTTTGTTCACAATAACGTCACTTCAATCC
  AGGGCATTCCTGGATTGGCTAGTGGTATAATTACAGAGAATCTTTGGAGTAATTACCTACCCAAGGGAGA
  GATCATCGAAAAGCCTTCAGCCAAATCTTCTGATGTCTTAGGCAGCTTAAACCATGAATATGTTCTTGTT
  GACATCACCACCTCCTTCACGCTTCTGGTGGGAATCTTCTTTCCCTCTGTTACAGGTATCATGGCTGGAT
  CAAACAGATCTGGAGATCTGAAAGATGCTCAGAAGTCTATTCCGATTGGTACTATCCTTGCCATCCTGAC
  CACCTCCTTTGTTTATTTAAGCAATGTTGTCCTTTTTGGTGCATGTATTGAAGGGGTTGTTCTCAGAGAC
  AAGTTCGGTGATGCTGTGAAAGGTAATTTGGTGGTAGGCACCTTATCTTGGCCATCCCCATGGGTGATTG
  TTATTGGCTCCTTCTTTTCAACATGTGGGGCTGGACTTCAGAGCCTCACAGGTGCACCGAGGCTGCTACA
  AGCTATTGCCAAGGATAACATCATACCGTTTCTGAGGGTTTTTGGCCACAGCAAAGCCAATGGGGAACCT
  ACCTGGGCTTTACTTCTAACTGCTGCCATTGCAGAGCTTGGAATACTCATTGCCTCCCTGGATCTTGTGG
  CCCCAATTCTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTAAACTTGGCATGTGCCTTGCAAACATT
  ACTTCGAACACCCAACTGGAGACCCCGATTCCGCTACTACCATTGGGCCCTTTCTTTCATGGGAATGAGT
  ATCTGTCTGGCTCTGATGTTCATTTCTTCCTGGTATTATGCCATTGTAGCCATGGTAATAGCTGGTATGA
  TCTACAAGTACATTGAATACCAAGGAGCTGAGAAAGAATGGGGTGATGGTATCCGTGGGCTGTCCCTCAG
  TGCAGCCCGGTTTGCTTTGCTTCGATTGGAGGAAGGACCTCCACACACTAAAAACTGGAGGCCTCAGTTG
  CTTGTATTACTGAAACTAGATGAAGACTTACATGTCAAGCATCCTCGCCTCCTCACCTTTGCCTCACAGC
  TCAAAGCAGGAAAAGGTCTCACTATTGTGGGCTCTGTCATCGTGGGGAACTTCCTAGAGAACTACGGTGA
  AGCTTTAGCTGCTGAGCAGACCATAAAGCACCTAATGGAGGCAGAGAAGGTAAAAGGATTCTGCCAGCTG
  GTGGTGGCCGCCAAGCTGAGAGAGGGCATTTCCCACCTCATCCAGTCATGTGGCCTTGGGGGCATGAAGC
  ACAACACGGTGGTGATGGGCTGGCCTAATGGCTGGCGTCAAAGCGAAGATGCCCGCGCTTGGAAGACTTT
  TATTGGCACAGTTCGAGTGACAACTGCTGCCCATCTTGCACTGCTGGTGGCTAAAAACATCTCCTTCTTT
  CCCAGCAATGTGGAGCAATTTTCTGAGGGCAACATTGATGTGTGGTGGATTGTGCATGATGGGGGGATGC
  TTATGCTACTACCATTCCTACTGAAACAGCACAAGGTGTGGCGAAAGTGCAGCATACGGATCTTCACAGT
  AGCCCAATTAGAAGACAACAGTATCCAAATGAAGAAGGACCTAGCCACCTTCCTATATCACTTACGCATT
  GAGGCGGAGGTAGAAGTGGTGGAGATGCATGACAGTGATATATCAGCATATACTTACGAGCGCACTTTGA
  TGATGGAACAAAGGTCCCAGATGCTTCGGCACATGCGGCTATCCAAAACAGAGCGAGACAGAGAGGCACA
  ATTGGTGAAAGACCGAAACTCAATGCTACGATTGACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAA
  ACCTATCAGGAGAAGGTGCACATGACTTGGACAAAAGACAAGTACATGGCATCCCGGGGACAAAAAGCGA
  AGTCAATGGAAGGATTCCAGGACCTGCTTAACATGCGTCCGGACCAGTCCAATGTGAGGCGGATGCATAC
  AGCAGTGAAACTCAACGAGGTTATAGTTAACAAGTCCCATGAAGCAAAGCTGGTTTTATTGAATATGCCA
  GGGCCACCCCGAAACCCTGAGGGTGATGAAAACTACATGGAGTTCCTAGAGGTGCTTACCGAGGGACTAG
  AGCGAGTCCTACTTGTCCGGGGTGGTGGCAGTGAAGTGATCACCATTTATTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209880 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEMSGATTSLATVALDPPSDRTSHPQDVIEDLSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYF
  DKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYPPCLQNI
  FGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVG
  LCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRY
  VNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKLWGFF
  CNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGSLNHEYVLV
  DITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRD
  KFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEP
  TWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMS
  ICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQL
  LVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQL
  VVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFF
  PSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRI
  EAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETE
  TYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMP
  GPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042495
• ORF Size: 3273 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042495.1, NP_001035960.1
• RefSeq Size: 7363 bp
• RefSeq ORF: 3276 bp
• Locus ID: 9990
• Protein Families: Transmembrane
• MW: 121.1 kDa
• Gene Summary: This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008]