ITPRIP (NM_033397) Human Tagged ORF Clone

CAT#: RC209883

ITPRIP (Myc-DDK-tagged)-Human inositol 1,4,5-trisphosphate receptor interacting protein (ITPRIP)

Product Images

Western blot validation of overexpression lysate (Cat# LY403247) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209883 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITPRIP
• Synonyms: bA127L20; bA127L20.2; DANGER; KIAA1754
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209883 representing NM_033397.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCATGGGGCTCTTCCGCGTGTGTCTGGTGGTGGTGACGGCCATCATCAACCACCCGCTGCTGTTC
  CCGCGGGAGAACGCCACAGTCCCCGAGAACGAGGAGGAGATCATCCGCAAGATGCAGGCGCACCAGGAG
  AAGCTGCAGCTGGAGCAGTTGCGCCTGGAGGAGGAGGTGGCTCGGCTGGCGGCCGAAAAGGAGGCACTG
  GAGCAGGTGGCGGAGGAGGGCAGGCAGCAGAACGAGACACGCGTGGCCTGGGACCTCTGGAGCACCCTC
  TGCATGATCCTCTTCCTGATGATCGAGGTGTGGCGGCAGGACCACCAGGAGGGGCCCTCACCTGAGTGC
  CTGGGCGGTGAGGAGGATGAGCTGCCTGGGCTGGGGGGCGCCCCCTTGCAGGGCCTCACCCTGCCCAAC
  AAGGCCACGCTTGGCCACTTTTATGAGCGCTGCATCCGGGGGGCCACGGCCGATGCAGCCCGTACCCGG
  GAGTTCCTGGAAGGCTTCGTGGATGACTTGCTGGAAGCCCTGAGGAGCCTCTGCAACCGGGACACCGAC
  ATGGAGGTGGAGGACTTCATTGGCGTGGACAGCATGTACGAGAACTGGCAGGTGGACAGGCCACTGCTG
  TGCCACCTTTTCGTGCCCTTCACACCCCCCGAGCCCTACCGCTTCCACCCAGAGCTCTGGTGCTCCGGC
  CGCTCAGTGCCCCTGGATCGCCAGGGCTACGGCCAGATCAAGGTGGTCCGCGCCGATGGGGACACATTG
  AGCTGCATCTGCGGCAAGACCAAGCTCGGGGAAGACATGCTGTGTCTCCTGCACGGCAGGAACAGCATG
  GCGCCTCCCTGCGGCGACATGGAGAACCTGCTGTGTGCCACAGATTCCCTGTACCTGGACACGATGCAG
  GTCATGAAGTGGTTCCAGACGGCCCTCACCAGAGCCTGGAAGGGCATCGCCCACAAGTACGAGTTCGAC
  CTGGCCTTTGGCCAGCTGGACAGCCCGGGGTCCCTGAAGATCAAGTTCCGTTCAGGGAAGTTCATGCCC
  TTCAACCTGATTCCTGTGATCCAGTGTGATGACTCGGACCTGTACTTTGTCTCCCACCTTCCCAGGGAG
  CCCTCTGAGGGCACCCCAGCCTCCAGCACAGACTGGCTCCTGTCCTTTGCTGTCTATGAGCGACACTTC
  CTCAGGACGACACTAAAGGCACTGCCCGAGGGCGCCTGCCACCTCAGCTGCCTGCAGATAGCATCCTTC
  CTGCTCTCCAAGCAGAGCCGCCTGACCGGTCCCAGCGGGCTCAGCAGCTACCACCTGAAGACGGCCCTA
  CTGCACCTCCTACTCCTCCGGCAGGCCGCCGACTGGAAGGCGGGGCAGCTGGACGCTCGTCTGCACGAG
  TTGCTGTGCTTCCTGGAGAAGAGCTTGCTCCAGAAGAAGCTCCACCACTTCTTCATCGGCAACCGCAAG
  GTGCCTGAGGCCATGGGACTCCCTGAGGCCGTGCTCAGGGCCGAGCCCCTCAACCTCTTCCGGCCCTTC
  GTCCTGCAGCGAAGCCTTTACCGTAAGACACTGGACTCCTTCTATGAGATGCTCAAGAATGCCCCAGCG
  CTCATTAGCGAGTATTCCCTACATGTCCCCTCAGACCAGCCTACCCCAAAAAGC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC209883
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAMGLFRVCLVVVTAIINHPLLFPRENATVPENEEEIIRKMQAHQEKLQLEQLRLEEEVARLAAEKEAL
  EQVAEEGRQQNETRVAWDLWSTLCMILFLMIEVWRQDHQEGPSPECLGGEEDELPGLGGAPLQGLTLPN
  KATLGHFYERCIRGATADAARTREFLEGFVDDLLEALRSLCNRDTDMEVEDFIGVDSMYENWQVDRPLL
  CHLFVPFTPPEPYRFHPELWCSGRSVPLDRQGYGQIKVVRADGDTLSCICGKTKLGEDMLCLLHGRNSM
  APPCGDMENLLCATDSLYLDTMQVMKWFQTALTRAWKGIAHKYEFDLAFGQLDSPGSLKIKFRSGKFMP
  FNLIPVIQCDDSDLYFVSHLPREPSEGTPASSTDWLLSFAVYERHFLRTTLKALPEGACHLSCLQIASF
  LLSKQSRLTGPSGLSSYHLKTALLHLLLLRQAADWKAGQLDARLHELLCFLEKSLLQKKLHHFFIGNRK
  VPEAMGLPEAVLRAEPLNLFRPFVLQRSLYRKTLDSFYEMLKNAPALISEYSLHVPSDQPTPKS
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033397
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033397.4
• RefSeq Size: 6825 bp
• RefSeq ORF: 1644 bp
• Locus ID: 85450
• UniProt ID: Q8IWB1
• Cytogenetics: 10q25.1
• MW: 62.1 kDa
• Gene Summary: This gene encodes a membrane-associated protein that binds the inositol 1,4,5-trisphosphate receptor (ITPR). The encoded protein enhances the sensitivity of ITPR to intracellular calcium signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]