P2Y11 (P2RY11) (NM_002566) Human Tagged ORF Clone

CAT#: RC210061

P2RY11 (Myc-DDK-tagged)-Human purinergic receptor P2Y, G-protein coupled, 11 (P2RY11)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2RY11
• Synonyms: P2Y11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210061 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCAACGTCTCGGGTGCCAAGTCCTGCCCTGCCAACTTCTTGGCAGCTGCCGACGACAAACTCA
  GTGGGTTCCAGGGGGACTTCCTGTGGCCCATACTGGTGGTTGAGTTCCTGGTGGCCGTGGCCAGCAATGG
  CCTGGCCCTGTACCGCTTCAGCATCCGGAAGCAGCGCCCATGGCACCCCGCCGTGGTCTTCTCTGTCCAG
  CTGGCAGTCAGCGACCTGCTCTGCGCTCTGACGCTGCCCCCGCTGGCCGCCTACCTCTATCCCCCCAAGC
  ACTGGCGCTATGGGGAGGCCGCGTGCCGCCTGGAGCGCTTCCTCTTCACCTGCAACCTGCTGGGCAGCGT
  CATCTTCATCACCTGCATCAGCCTCAACCGCTACCTGGGCATCGTGCACCCCTTCTTCGCCCGAAGCCAC
  CTGCGACCCAAGCACGCCTGGGCCGTGAGCGCTGCCGGCTGGGTCCTGGCCGCCCTGCTGGCCATGCCCA
  CACTCAGCTTCTCCCACCTGAAGAGGCCGCAGCAGGGGGCGGGCAACTGCAGCGTGGCCAGGCCCGAGGC
  CTGCATCAAGTGTCTGGGGACAGCAGACCACGGGCTGGCGGCCTACAGAGCGTATAGCCTGGTGCTGGCG
  GGGTTGGGCTGCGGCCTGCCGCTGCTGCTCACGCTGGCAGCCTACGGCGCCCTCGGGCGGGCCGTGCTAC
  GCAGCCCAGGCATGACTGTGGCCGAGAAGCTGCGTGTGGCAGCGTTGGTGGCCAGTGGTGTGGCCCTCTA
  CGCCAGCTCCTATGTGCCCTACCACATCATGCGGGTGCTCAACGTGGATGCTCGGCGGCGCTGGAGCACC
  CGCTGCCCGAGCTTTGCAGACATAGCCCAGGCCACAGCAGCCCTGGAGCTGGGGCCCTACGTGGGCTACC
  AGGTGATGCGGGGCCTCATGCCCCTGGCCTTCTGTGTCCACCCTCTACTCTACATGGCCGCAGTGCCCAG
  CCTGGGCTGCTGCTGCCGACACTGCCCCGGCTACAGGGACAGCTGGAACCCAGAGGACGCCAAGAGCACT
  GGCCAAGCCCTGCCCCTCAATGCCACAGCCGCCCCTAAACCGTCAGAGCCCCAGTCCCGTGAGCTGAGCC
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210061 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAANVSGAKSCPANFLAAADDKLSGFQGDFLWPILVVEFLVAVASNGLALYRFSIRKQRPWHPAVVFSVQ
  LAVSDLLCALTLPPLAAYLYPPKHWRYGEAACRLERFLFTCNLLGSVIFITCISLNRYLGIVHPFFARSH
  LRPKHAWAVSAAGWVLAALLAMPTLSFSHLKRPQQGAGNCSVARPEACIKCLGTADHGLAAYRAYSLVLA
  GLGCGLPLLLTLAAYGALGRAVLRSPGMTVAEKLRVAALVASGVALYASSYVPYHIMRVLNVDARRRWST
  RCPSFADIAQATAALELGPYVGYQVMRGLMPLAFCVHPLLYMAAVPSLGCCCRHCPGYRDSWNPEDAKST
  GQALPLNATAAPKPSEPQSRELSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002566
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002566.2, NM_002566.3, NM_002566.4, NP_002557.2
• RefSeq Size: 1977 bp
• RefSeq ORF: 1125 bp
• Locus ID: 5032
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 40.3 kDa
• Gene Summary: The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is coupled to the stimulation of the phosphoinositide and adenylyl cyclase pathways and behaves as a selective purinoceptor. Naturally occuring read-through transcripts, resulting from intergenic splicing between this gene and an immediately upstream gene (PPAN, encoding peter pan homolog), have been found. The PPAN-P2RY11 read-through mRNA is ubiquitously expressed and encodes a fusion protein that shares identity with each individual gene product. [provided by RefSeq, Jul 2008]