PPM1J (NM_005167) Human Tagged ORF Clone

CAT#: RC210062

PPM1J (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1J (PPM1J)

Product Images

Western blot validation of overexpression lysate (Cat# LY417470) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210062 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PPM1J protein (Cat# TP310062). The protein was produced from HEK293T cells transfected with PPM1J cDNA clone (Cat# RC210062) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPM1J
• Synonyms: PP2CZ; PP2Czeta; PPP2CZ
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210062 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAAACCGGGTGCGCTCGGCCGTGGCGCACCTGGTGAGCTCCGGGGGCGCTCCGCCTCCGCGCCCCA
  AATCCCCGGACCTGCCCAACGCCGCCTCGGCGCCGCCCGCCGCCGCTCCAGAAGCGCCCAGGAGCCCTCC
  CGCGAAGGCTGGGAGCGGGAGCGCGACGCCCGCGAAGGCTGTTGAGGCTCGAGCGAGCTTCTCCAGACCG
  ACCTTTCTGCAGCTGAGCCCCGGGGGGCTGCGACGCGCCGATGACCACGCGGGCCGGGCTGTGCAAAGCC
  CCCCGGACACGGGCCGCCGCCTGCCCTGGAGCACAGGCTACGCCGAGGTCATCAATGCTGGCAAGAGTCG
  GCACAATGAGGACCAGGCTTGCTGTGAAGTGGTGTATGTGGAAGGTCGGAGGAGTGTTACAGGAGTACCT
  AGGGAGCCTAGCCGAGGCCAGGGACTCTGCTTCTACTACTGGGGCCTATTTGATGGGCATGCAGGGGGCG
  GAGCTGCTGAAATGGCCTCACGGCTCCTGCATCGCCATATCCGAGAGCAGCTAAAGGACCTGGTAGAGAT
  ACTTCAGGACCCTTCGCCACCACCCCTCTGCCTCCCAACCACTCCGGGGACCCCAGATTCCTCCGATCCC
  TCTCACTTGCTTGGCCCTCAGTCCTGCTGGTCTTCACAGAAGGAAGTGAGCCACGAGAGCCTGGTAGTGG
  GGGCCATTGAGAATGCCTTCCAGCTCATGGATGAGCAGATGGCCCGGGAGCGGCGTGGCCACCAAGTGGA
  GGGGGGCTGCTGTGCACTGGTTGTGATCTACCTGCTAGGCAAGGTGTACGTGGCCAATGCAGGCGATAGC
  AGGGCCATCATTGTCCGGAATGGTGAAATCATTCCAATGTCCCGGGAGTTTACCCCGGAGACTGAGCGCC
  AGCGTCTTCAGCTGCTTGGCTTCCTGAAACCAGAGCTGCTAGGCAGTGAATTCACCCACCTTGAGTTCCC
  CCGCAGAGTTCTGCCCAAGGAGCTGGGGCAGAGGATGTTGTACCGGGACCAGAACATGACCGGCTGGGCC
  TACAAAAAGATCGAGCTGGAGGATCTCAGGTTTCCTCTGGTCTGTGGGGAGGGCAAAAAGGCTCGGGTGA
  TGGCCACCATTGGGGTGACCCGAGGCTTGGGAGACCACAGCCTTAAGGTCTGCAGTTCCACCCTGCCCAT
  CAAGCCCTTTCTCTCCTGCTTCCCTGAGGTACGAGTGTATGACCTGACACAATATGAGCACTGCCCAGAT
  GATGTGCTAGTCCTGGGAACAGATGGCCTGTGGGATGTCACTACTGACTGTGAGGTAGCTGCCACTGTGG
  ACAGGGTGCTGTCGGCCTATGAGCCTAATGACCACAGCAGGTATACAGCTCTGGCCCAAGCTCTGGTCCT
  GGGGGCCCGGGGTACCCCCCGAGACCGTGGCTGGCGTCTCCCCAACAACAAGCTGGGTTCCGGGGATGAC
  ATCTCTGTCTTCGTCATCCCCCTGGGAGGGCCAGGCAGTTACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210062 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLNRVRSAVAHLVSSGGAPPPRPKSPDLPNAASAPPAAAPEAPRSPPAKAGSGSATPAKAVEARASFSRP
  TFLQLSPGGLRRADDHAGRAVQSPPDTGRRLPWSTGYAEVINAGKSRHNEDQACCEVVYVEGRRSVTGVP
  REPSRGQGLCFYYWGLFDGHAGGGAAEMASRLLHRHIREQLKDLVEILQDPSPPPLCLPTTPGTPDSSDP
  SHLLGPQSCWSSQKEVSHESLVVGAIENAFQLMDEQMARERRGHQVEGGCCALVVIYLLGKVYVANAGDS
  RAIIVRNGEIIPMSREFTPETERQRLQLLGFLKPELLGSEFTHLEFPRRVLPKELGQRMLYRDQNMTGWA
  YKKIELEDLRFPLVCGEGKKARVMATIGVTRGLGDHSLKVCSSTLPIKPFLSCFPEVRVYDLTQYEHCPD
  DVLVLGTDGLWDVTTDCEVAATVDRVLSAYEPNDHSRYTALAQALVLGARGTPRDRGWRLPNNKLGSGDD
  ISVFVIPLGGPGSYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005167
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005167.5, NP_005158.5
• RefSeq Size: 1762 bp
• RefSeq ORF: 1518 bp
• Locus ID: 333926
• Domains: PP2C
• Protein Families: Phosphatase
• MW: 54.8 kDa
• Gene Summary: This gene encodes the serine/threonine protein phosphatase. The mouse homolog of this gene apparently belongs to the protein phosphatase 2C family of genes. The exact function of this gene is not yet known. [provided by RefSeq, Jul 2008]