CER1 (NM_005454) Human Tagged ORF Clone

CAT#: RC210111

CER1 (Myc-DDK-tagged) - Human cerberus 1, DAN family BMP antagonist (CER1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417294) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210111 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CER1
• Synonyms: DAND4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210111 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTCCTCTTATTTCAGCTGCTGGTACTCCTGCCTCTAGGAAAGACCACACGGCACCAGGATGGCC
  GCCAGAATCAGAGTTCTCTTTCCCCCGTACTCCTGCCAAGGAATCAAAGAGAGCTTCCCACAGGCAACCA
  TGAGGAAGCTGAGGAGAAGCCAGATCTGTTTGTCGCAGTGCCACACCTTGTAGGCACCAGCCCTGCAGGG
  GAAGGCCAGAGGCAGAGAGAGAAGATGCTGTCCAGATTTGGCAGGTTCTGGAAGAAGCCTGAGAGAGAAA
  TGCATCCATCCAGGGACTCAGATAGTGAGCCCTTCCCACCTGGGACCCAGTCCCTCATCCAGCCGATAGA
  TGGAATGAAAATGGAGAAATCTCCTCTTCGGGAAGAAGCCAAGAAATTCTGGCACCACTTCATGTTCAGA
  AAAACTCCGGCTTCTCAGGGGGTCATCTTGCCCATCAAAAGCCATGAAGTACATTGGGAGACCTGCAGGA
  CAGTGCCCTTCAGCCAGACTATAACCCACGAAGGCTGTGAGAAAGTAGTTGTTCAGAACAACCTTTGCTT
  TGGGAAATGCGGGTCTGTTCATTTTCCTGGAGCCGCGCAGCACTCCCATACCTCCTGCTCTCACTGTTTG
  CCTGCCAAGTTCACCACGATGCACTTGCCAGTGAACTGCACTGAACTTTCCTCCGTGATCAAGGTGGTGA
  TGCTGGTGGAGGAGTGCCAGTGCAAGGTGAAGACGGAGCATGAAGATGGACACATCCTACATGCTGGCTC
  CCAGGATTCCTTTATCCCAGGAGTTTCAGCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210111 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHLLLFQLLVLLPLGKTTRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTSPAG
  EGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFMFR
  KTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKVVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCL
  PAKFTTMHLPVNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005454
• ORF Size: 803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005454.1, NM_005454.2, NP_005445.1
• RefSeq Size: 1206 bp
• RefSeq ORF: 804 bp
• Locus ID: 9350
• Protein Families: Secreted Protein
• Protein Pathways: Wnt signaling pathway
• MW: 30.1 kDa
• Gene Summary: This gene encodes a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. [provided by RefSeq, Jul 2008]