GPRC5D (NM_018654) Human Tagged ORF Clone

CAT#: RC210138

GPRC5D (Myc-DDK-tagged)-Human G protein-coupled receptor, family C, group 5, member D (GPRC5D)

Product Images

Western blot validation of overexpression lysate (Cat# LY412961) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210138 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPRC5D
• Synonyms: MGC129713; MGC129714
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210138 representing NM_018654
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAAGGACTGCATCGAGTCCACTGGAGACTATTTTCTTCTCTGTGACGCCGAGGGGCCATGGGGCA
  TCATTCTGGAGTCCCTGGCCATACTTGGCATCGTGGTCACAATTCTGCTACTCTTAGCATTTCTCTTCCT
  CATGCGAAAGATCCAAGACTGCAGCCAGTGGAATGTCCTCCCCACCCAGCTCCTCTTCCTCCTGAGTGTC
  CTGGGGCTCTTCGGACTCGCTTTTGCCTTCATCATCGAGCTCAATCAACAAACTGCCCCCGTACGCTACT
  TTCTCTTTGGGGTTCTCTTTGCTCTCTGTTTCTCATGCCTCTTAGCTCATGCCTCCAATCTAGTGAAGCT
  GGTTCGGGGTTGTGTCTCCTTCTCCTGGACGACAATTCTGTGCATTGCTATTGGTTGCAGTCTGTTGCAA
  ATCATTATTGCCACTGAGTATGTGACTCTCATCATGACCAGAGGTATGATGTTTGTGAATATGACACCCT
  GCCAGCTCAATGTGGACTTTGTTGTACTCCTGGTCTATGTCCTCTTCCTGATGGCCCTCACATTCTTCGT
  CTCCAAAGCCACCTTCTGTGGCCCGTGTGAGAACTGGAAGCAGCATGGAAGGCTCATCTTTATCACTGTG
  CTCTTCTCCATCATCATCTGGGTGGTGTGGATCTCCATGCTCCTGAGAGGCAACCCGCAGTTCCAGCGAC
  AGCCCCAGTGGGACGACCCGGTCGTCTGCATTGCTCTGGTCACCAACGCATGGGTTTTCCTGCTGCTGTA
  CATCGTCCCTGAGCTCTGCATTCTCTACAGATCGTGTAGACAGGAGTGCCCTTTACAAGGCAATGCCTGC
  CCCGTCACAGCCTACCAACACAGCTTCCAAGTGGAGAACCAGGAGCTCTCCAGAGCCCGAGACAGTGATG
  GAGCTGAGGAGGATGTAGCATTAACTTCATATGGTACTCCCATTCAGCCGCAGACTGTTGATCCCACACA
  AGAGTGTTTCATCCCACAGGCTAAACTAAGCCCCCAGCAAGATGCAGGAGGAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210138 representing NM_018654
  Red=Cloning site Green=Tags(s)
  
  MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSV
  LGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQ
  IIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITV
  LFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNAC
  PVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018654
• ORF Size: 1035 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018654.1, NP_061124.1
• RefSeq Size: 1038
• RefSeq ORF: 1038
• Locus ID: 55507
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 38.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined. [provided by RefSeq, Jul 2008]