NSUN5C (NM_032158) Human Tagged ORF Clone

CAT#: RC210254

NSUN5C (Myc-DDK-tagged)-Human NOL1/NOP2/Sun domain family, member 5C (NSUN5C), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410311) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210254 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NSUN5C
• Synonyms: DKFZp434K058; DKFZp666P104; FLJ11626; MGC15057; MGC129801; NOL1/NOP2/Sun domain family, member 5C; NOL1R2; WBSCR20B; WBSCR20C; Williams Beuren syndrome chromosome region 20C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210254 representing NM_032158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGAGCTGCTGGTGTTTCCCGCCCAGACAGATCTGCATGAACACCCACTGTACCGGGCCGGACACC
  TCATTCTGCAGGACAGGGCCAGCTGTCTCCCAGCCATGCTGCTGGACCCCCGCCAGGCTCCCATGTCATG
  GATGCCTGTGCCACCCCAGGCAATAAAGACCAGTCACTTGGCTGCTCTTCTGAAGAACCAAGGGAAGATC
  TTTGCCTTTGACCTGGATGCCAGGCGGCTGGCATCCATGGCCACGCTGCTGGCCTGGGCTGGCGTCTCCT
  GCTGTGAGCTGGCTGAGGAGGACTTCCTGGCGGTCTCCCCCTTAGATCCGCGCTATCGTGAGGTCCACTA
  TGTCCTGCTGGATCCTTCCTGCAGTGGCTCGGGTATGCCGAGCAGACAGCTGGAGGAGCCCGGGGCAGGG
  ACACCTAGCCCGGTGCGTCTGCATGCCCTGGCAGGGTTCCAGCAGCGAGCCCTGTGCCACGCGCTCACTT
  TCCCTTCCCTGCAGCGGCTCGTCTACTCCATGTGCTCCCTCTGCCAGGAGGAGAATGAAGACATGGTACA
  AGATGCGCTGCAGCAGAACCCGGGCGCCTTCAGGCTAGCTCCCGCCCTGCCTGCCCGGCCCCACCGAGGC
  CTGAGCACGTTCCCGGGTGCCGAGCACTGCCTCCGGGCTTCCCCCAAGACCACGCTTAGCGGTGGCTTCT
  TCGTTGCTGTAATTGAACGGGTCGAGATGCCGACCTCAGCCTCACAGGCCAAAGCATCAGCACCAGAACG
  CACACCCAGCCCAGCCCCAAAGAGAAAGAAGAGAGCAAAAAGCTGCAGCCGGTGCTTGCACACCGCCTTG
  CACATAGCAGAGGCTCCGGGCTCACTCCTTCCTGGTGGGAAAGGAAGATGCCTGTCCTCTCCGTGGAAGA
  CCCTGGGCCCTCACCGCAGGCAGCAGTTTGCGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210254 representing NM_032158
  Red=Cloning site Green=Tags(s)
  
  MPELLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPRQAPMSWMPVPPQAIKTSHLAALLKNQGKI
  FAFDLDARRLASMATLLAWAGVSCCELAEEDFLAVSPLDPRYREVHYVLLDPSCSGSGMPSRQLEEPGAG
  TPSPVRLHALAGFQQRALCHALTFPSLQRLVYSMCSLCQEENEDMVQDALQQNPGAFRLAPALPARPHRG
  LSTFPGAEHCLRASPKTTLSGGFFVAVIERVEMPTSASQAKASAPERTPSPAPKRKKRAKSCSRCLHTAL
  HIAEAPGSLLPGGKGRCLSSPWKTLGPHRRQQFAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032158
• ORF Size: 945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032158.3, NP_115534.2
• RefSeq Size: 1760
• RefSeq ORF: 947
• Locus ID: 260294
• MW: 34.2 kDa
• Gene Summary: This locus represents a transcribed pseudogene of a nearby locus on chromosome 7, which encodes a putative methyltransferase. There is also a third closely related pseudogene locus in this region. There is extensive alternative splicing at this locus. [provided by RefSeq, Jul 2013]