WDR8 (WRAP73) (NM_017818) Human Tagged ORF Clone

CAT#: RC210272

WRAP73 (Myc-DDK-tagged)-Human WD repeat containing, antisense to TP73 (WRAP73)

Product Images

Western blot validation of overexpression lysate (Cat# LY413527) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210272 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WRAP73 protein (Cat# TP310272). The protein was produced from HEK293T cells transfected with WRAP73 cDNA clone (Cat# RC210272) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WRAP73
• Synonyms: WDR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210272 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTCTCCGAGGTATTCAAGCTCTCCAGCTTACTCTGCAAGTTCTCCCCGGACGGCAAGTACCTGG
  CTTCCTGTGTCCAGTACCGGTTAGTGGTCCGGGATGTGAACACCCTTCAGATCCTTCAGCTGTACACGTG
  CCTAGACCAGATCCAGCACATCGAGTGGTCGGCAGACTCGCTCTTCATCCTGTGCGCCATGTACAAGCGA
  GGGCTGGTGCAGGTCTGGTCTTTAGAGCAGCCCGAATGGCACTGCAAAATAGACGAGGGCTCAGCCGGGC
  TGGTGGCCTCGTGCTGGAGCCCGGACGGGCGCCACATTCTCAACACCACGGAATTCCATCTGCGGATAAC
  CGTCTGGTCCTTGTGCACAAAATCCGTGTCTTACATCAAATACCCGAAAGCTTGTCTGCAGGGAATCACC
  TTCACCAGGGACGGCCGCTACATGGCGCTGGCAGAACGGCGCGACTGCAAAGATTACGTGAGCATCTTCG
  TCTGCAGTGATTGGCAGCTCCTGCGGCATTTTGATACGGACACCCAGGATCTCACAGGGATTGAGTGGGC
  CCCAAACGGCTGTGTGCTGGCAGTGTGGGACACCTGCTTGGAGTACAAGATTCTGCTGTACTCATTGGAT
  GGCCGGTTGTTGTCCACGTACAGCGCTTACGAGTGGTCCCTGGGCATCAAGTCTGTGGCCTGGAGCCCCA
  GCAGTCAGTTCCTGGCAGTTGGGAGCTATGATGGAAAGGTGCGCATCCTTAATCACGTGACTTGGAAAAT
  GATCACGGAGTTTGGGCATCCTGCAGCCATTAATGATCCCAAGATAGTGGTGTATAAGGAGGCCGAGAAG
  AGCCCACAGCTGGGACTGGGCTGCCTCTCCTTCCCGCCGCCCCGGGCCGGGGCCGGCCCTCTCCCGAGCT
  CAGAGAGTAAATATGAGATCGCCTCTGTCCCAGTCTCCTTACAGACACTGAAACCTGTTACCGACAGAGC
  AAACCCGAAAATCGGCATAGGAATGCTGGCATTTAGTCCTGACAGCTACTTCCTGGCGACAAGGAACGAC
  AACATTCCCAATGCCGTCTGGGTCTGGGACATTCAGAAGCTGAGGCTGTTCGCGGTGCTCGAGCAGCTGT
  CCCCAGTGCGCGCATTTCAGTGGGACCCGCAGCAGCCGCGGCTGGCCATCTGCACGGGAGGCAGCAGGCT
  CTACCTGTGGTCCCCAGCGGGCTGCATGTCGGTGCAGGTGCCTGGGGAAGGCGACTTTGCAGTGCTCTCT
  CTGTGCTGGCATTTAAGCGGAGACTCGATGGCCCTCCTCAGCAAGGATCACTTCTGCCTCTGCTTCCTGG
  AGACAGAGGCAGTGGTCGGCACAGCCTGCAGACAGCTGGGCGGCCACACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210272 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNFSEVFKLSSLLCKFSPDGKYLASCVQYRLVVRDVNTLQILQLYTCLDQIQHIEWSADSLFILCAMYKR
  GLVQVWSLEQPEWHCKIDEGSAGLVASCWSPDGRHILNTTEFHLRITVWSLCTKSVSYIKYPKACLQGIT
  FTRDGRYMALAERRDCKDYVSIFVCSDWQLLRHFDTDTQDLTGIEWAPNGCVLAVWDTCLEYKILLYSLD
  GRLLSTYSAYEWSLGIKSVAWSPSSQFLAVGSYDGKVRILNHVTWKMITEFGHPAAINDPKIVVYKEAEK
  SPQLGLGCLSFPPPRAGAGPLPSSESKYEIASVPVSLQTLKPVTDRANPKIGIGMLAFSPDSYFLATRND
  NIPNAVWVWDIQKLRLFAVLEQLSPVRAFQWDPQQPRLAICTGGSRLYLWSPAGCMSVQVPGEGDFAVLS
  LCWHLSGDSMALLSKDHFCLCFLETEAVVGTACRQLGGHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017818
• ORF Size: 1380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017818.1, NM_017818.2, NM_017818.3, NP_060288.2
• RefSeq Size: 1708
• RefSeq ORF: 1383
• Locus ID: 49856
• Domains: WD40
• Protein Families: Stem cell - Pluripotency
• MW: 51.6 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Studies of the related mouse protein suggest that the encoded protein may play a role in the process of ossification. [provided by RefSeq, Mar 2009]