Alpha Taxilin (TXLNA) (NM_175852) Human Tagged ORF Clone

CAT#: RC210301

TXLNA (Myc-DDK-tagged)-Human taxilin alpha (TXLNA)

Product Images

Western blot validation of overexpression lysate (Cat# LY406231) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210301 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TXLNA protein (Cat# TP310301). The protein was produced from HEK293T cells transfected with TXLNA cDNA clone (Cat# RC210301) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TXLNA
• Synonyms: IL14; TXLN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210301 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAACCAAGACAAAAAGAACGGGGCTGCCAAACAATCCAATCCAAAAAGCAGCCCAGGACAACCGG
  AAGCAGGACCCGAGGGAGCCCAGGAGCGGCCCAGCCAGGCGGCTCCTGCAGTAGAAGCAGAAGGTCCCGG
  CAGCAGCCAGGCTCCTCGGAAGCCGGAGGGGGCTCAAGCCAGAACGGCTCAGTCTGGGGCCCTTCGTGAT
  GTCTCTGAGGAGCTGAGCCGCCAACTGGAAGACATACTGAGCACATACTGTGTGGACAATAACCAGGGGG
  GCCCCGGCGAGGATGGGGCACAGGGTGAGCCGGCTGAACCCGAAGATGCAGAGAAGTCCCGGACCTATGT
  GGCAAGGAATGGGGAGCCTGAACCAACTCCAGTAGTCAATGGAGAGAAGGAACCCTCCAAGGGGGATCCA
  AACACAGAAGAGATCCGGCAGAGTGACGAGGTCGGAGACCGAGACCATCGAAGGCCACAGGAGAAGAAAA
  AAGCCAAGGGTTTGGGGAAGGAGATCACGTTGCTGATGCAGACATTGAATACTCTGAGTACCCCAGAGGA
  GAAGCTGGCTGCTCTGTGCAAGAAGTATGCTGAACTGCTGGAGGAGCACCGGAATTCACAGAAGCAGATG
  AAGCTCCTACAGAAAAAGCAGAGCCAGCTGGTGCAAGAGAAGGACCACCTGTGCGGTGAGCACAGCAAGG
  CCGTCCTGGCCCGCAGCAAGCTTGAGAGCCTATGCCGTGAGCTGCAGCGGCACAACCGCTCCCTCAAGGA
  AGAAGGTGTGCAGCGGGCCCGGGAGGAGGAGGAGAAGCGCAAGGAGGTGACCTCGCACTTCCAGGTGACA
  CTGAATGACATTCAGCTGCAGATGGAACAGCACAATGAGCGCAACTCCAAGCTGCGCCAAGAGAACATGG
  AGCTGGCTGAGAGGCTCAAGAAGCTGATTGAGCAGTATGAGCTGCGCGAGGAGCATATCGACAAAGTCTT
  CAAACACAAGGACCTACAACAGCAGCTGGTGGATGCCAAGCTCCAGCAGGCCCAGGAGATGCTAAAGGAG
  GCAGAAGAGCGGCACCAGCGGGAGAAGGATTTTCTCCTGAAAGAGGCAGTAGAGTCCCAGAGGATGTGTG
  AGCTGATGAAGCAGCAAGAGACCCACCTGAAGCAACAGCTTGCCCTATACACAGAGAAGTTTGAGGAGTT
  CCAGAACACACTTTCCAAAAGCAGCGAGGTATTCACCACATTCAAGCAGGAGATGGAAAAGATGACTAAG
  AAGATCAAGAAGCTGGAGAAAGAAACCACCATGTACCGGTCCCGGTGGGAGAGCAGCAACAAGGCCCTGC
  TTGAGATGGCTGAGGAGAAAACAGTCCGGGATAAAGAACTGGAGGGCCTGCAGGTAAAAATCCAACGGCT
  GGAGAAGCTGTGCCGGGCACTGCAGACAGAGCGCAATGACCTGAACAAGAGGGTACAGGACCTGAGTGCT
  GGTGGCCAGGGCTCCCTCACTGACAGTGGCCCTGAGAGGAGGCCAGAGGGGCCTGGGGCTCAAGCACCCA
  GCTCCCCCAGGGTCACAGAAGCGCCTTGCTACCCAGGAGCACCGAGCACAGAAGCATCAGGCCAGACTGG
  GCCTCAAGAGCCCACCTCCGCCAGGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210301 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKNQDKKNGAAKQSNPKSSPGQPEAGPEGAQERPSQAAPAVEAEGPGSSQAPRKPEGAQARTAQSGALRD
  VSEELSRQLEDILSTYCVDNNQGGPGEDGAQGEPAEPEDAEKSRTYVARNGEPEPTPVVNGEKEPSKGDP
  NTEEIRQSDEVGDRDHRRPQEKKKAKGLGKEITLLMQTLNTLSTPEEKLAALCKKYAELLEEHRNSQKQM
  KLLQKKQSQLVQEKDHLCGEHSKAVLARSKLESLCRELQRHNRSLKEEGVQRAREEEEKRKEVTSHFQVT
  LNDIQLQMEQHNERNSKLRQENMELAERLKKLIEQYELREEHIDKVFKHKDLQQQLVDAKLQQAQEMLKE
  AEERHQREKDFLLKEAVESQRMCELMKQQETHLKQQLALYTEKFEEFQNTLSKSSEVFTTFKQEMEKMTK
  KIKKLEKETTMYRSRWESSNKALLEMAEEKTVRDKELEGLQVKIQRLEKLCRALQTERNDLNKRVQDLSA
  GGQGSLTDSGPERRPEGPGAQAPSSPRVTEAPCYPGAPSTEASGQTGPQEPTSARA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175852
• ORF Size: 1638 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175852.1, NM_175852.2, NM_175852.3, NP_787048.1
• RefSeq Size: 4843 bp
• RefSeq ORF: 1641 bp
• Locus ID: 200081
• Protein Families: Druggable Genome
• MW: 61.8 kDa
• Gene Summary: May be involved in intracellular vesicle traffic and potentially in calcium-dependent exocytosis in neuroendocrine cells. [UniProtKB/Swiss-Prot Function]