KCNE3 (NM_005472) Human Tagged ORF Clone
CAT#: RC210303
KCNE3 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3)
"NM_005472" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KCNE3 |
Synonyms | BRGDA6; HOKPP; HYPP; MiRP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210303 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTACCAATGGAACGGAGACCTGGTATGAGAGCCTGCATGCCGTGCTGAAGGCTCTAAATGCCA CTCTTCACAGCAATTTGCTCTGCCGGCCAGGGCCAGGGCTGGGGCCAGACAACCAGACTGAAGAGAGGCG GGCCAGCCTACCTGGCCGTGATGACAACTCCTACATGTACATTCTCTTTGTCATGTTTCTATTTGCTGTA ACTGTGGGCAGCCTCATCCTGGGATACACCCGCTCCCGCAAAGTGGACAAGCGTAGTGACCCCTATCATG TGTATATCAAGAACCGTGTGTCTATGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210303 protein sequence
Red=Cloning site Green=Tags(s) METTNGTETWYESLHAVLKALNATLHSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMYILFVMFLFAV TVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005472 |
ORF Size | 309 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005472.1, NM_005472.2, NM_005472.3, NM_005472.4, NP_005463.1 |
RefSeq Size | 3070 bp |
RefSeq ORF | 312 bp |
Locus ID | 10008 |
Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
MW | 11.7 kDa |
Gene Summary | Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, isk-related subfamily. This member is a type I membrane protein, and a beta subunit that assembles with a potassium channel alpha-subunit to modulate the gating kinetics and enhance stability of the multimeric complex. This gene is prominently expressed in the kidney. A missense mutation in this gene is associated with hypokalemic periodic paralysis. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC111131 | KCNE3 (untagged)-Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3) |
USD 420.00 |
|
RG210303 | KCNE3 (GFP-tagged) - Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3) |
USD 460.00 |
|
RC210303L1 | Lenti ORF clone of Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3), Myc-DDK-tagged |
USD 768.00 |
|
RC210303L2 | Lenti ORF clone of Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3), mGFP tagged |
USD 620.00 |
|
RC210303L3 | Lenti ORF clone of Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3), Myc-DDK-tagged |
USD 620.00 |
|
RC210303L4 | Lenti ORF clone of Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3), mGFP tagged |
USD 620.00 |
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