PI 3 Kinase regulatory subunit 4 (PIK3R4) (NM_014602) Human Tagged ORF Clone

CAT#: RC210308

PIK3R4 (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4)

Product Images

Western blot validation of overexpression lysate (Cat# LY415157) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210308 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PIK3R4 protein (Cat# TP310308). The protein was produced from HEK293T cells transfected with PIK3R4 cDNA clone (Cat# RC210308) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIK3R4
• Synonyms: p150; VPS15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210308 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCTTTCTGTAGAGAGTTATTTTTCAGATATTC
  ATGACTTTGAATATGATAAAAGCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGA
  AGGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCCTTTAACCAGCTATAAACAAGAG
  CTGGAGGAACTGAAAATCAGGCTTAATTCTGCACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAG
  CATCTGAGAAAGCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCATCAGTACCCG
  TCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCCAGATCCTGACAGCTGTGGACCAAGCACAC
  AAATCTGGAGTTCGTCATGGGGACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCTTC
  TAACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCGGCAGATTTCAATTATTTCTT
  TGACACATCACGGAGGAGAACTTGCTATATTGCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACT
  GAGTTAGAATATATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAACAAGAGGAG
  AGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTGATAGCTGAGCTTTTTACAGAAGGTGTACC
  ATTATTTGATCTCTCTCAACTTTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAA
  ATTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAGCCAGATAAACGTTTAGAGG
  CAGAAGATTACTTAAAACAGCAGCGTGGCAATGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTA
  CATGGCCCAGTTTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGGAAGGATTTG
  GGCAACATTATTCACAATCTCTGTGGACATGATCTGCCAGAAAAAGCCGAAGGAGAGCCTAAGGAAAATG
  GGCTGGTTATCTTGGTATCTGTTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTAGC
  TGCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAATCCTTTTGGATCGTATTACTCCA
  TATCTTTTGCATTTCAGCAATGACTCTGTTCCTAGGGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAG
  TTCTTGCTCTCGTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACATTCTGCCAGGCAT
  AGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTAGCCTATGCTGAAAACATAGCTCTGCTGGCA
  GAAACAGCTCTGAGATTCCTGGAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAATG
  AAGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTCCAAGCCTTACATGAAATGGT
  CCAGCAGAAAGTTGTTACTTTGCTAAGTGACCCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGA
  ATAACACGGCTGTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCACATGATTACTT
  TCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATTTTTTGATAGTATAGTTGGTGTTGCTGCCTA
  TGTTGGCTGGCAAAGCTCCTCAATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTT
  GTCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACTGCTACAAAAACCCCATGTTT
  ACGAATTTGCCAGTGATATTGCCCCCTTCCTGTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGG
  ATTTATCACAGTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTACTGTAAACTGATGCCTTATCTTGAC
  CCATATATTACCCAACCAATAATACAGATTGAAAGAAAACTTGTTCTGCTCAGTGTTTTAAAGGAACCAG
  TAAGTCGTTCTATATTTGATTATGCTTTGAGGTCTAAAGATATTACTAGCTTGTTCAGACATCTTCACAT
  GCGTCAGAAGAAACGAAATGGTTCTCTTCCCGACTGCCCTCCGCCAGAGGATCCTGCCATAGCACAGCTT
  CTGAAGAAGTTGCTCTCACAGGGAATGACAGAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGACTTCA
  TGATGAAATCTAATAAAGCAAAGGCCAATATAGTGGACCAGAGCCATCTTCATGATAGTAGTCAGAAAGG
  TGTAATTGACTTGGCAGCTTTAGGCATAACTGGGAGACAAGTTGATCTTGTTAAAACCAAACAAGAACCA
  GATGACAAACGGGCCAGAAAACATGTAAAACAAGACTCAAATGTAAATGAAGAATGGAAAAGCATGTTTG
  GGTCACTGGACCCACCAAACATGCCACAGGCCCTACCTAAAGGGAGTGATCAGGAGGTGATTCAGACTGG
  GAAACCTCCTCGTTCCGAGTCCTCTGCTGGCATTTGTGTCCCTTTGTCAACTTCTTCACAGGTTCCAGAA
  GTGACAACTGTCCAAAATAAAAAACCAGTAATACCGGTTTTAAGTAGTACAATCTTACCATCCACCTATC
  AGATTCGAATTACAACTTGTAAAACTGAACTTCAGCAACTCATCCAGCAAAAGCGGGAGCAGTGCAATGC
  TGAGAGAATAGCTAAGCAGATGATGGAAAATGCTGAATGGGAGAGTAAACCACCACCACCTGGATGGCGT
  CCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCATAAATCTGCTGTGAATCGAATTAGAGTCTCTGATG
  AACACTCACTTTTTGCAACATGTTCAAATGATGGCACAGTGAAAATCTGGAACAGTCAAAAGATGGAGGG
  GAAGACCACCACTACCAGATCTATTCTTACATACAGCCGAATTGGAGGACGAGTCAAGACGCTCACATTC
  TGCCAAGGCTCCCACTATTTAGCCATAGCATCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAGGCTT
  CTAAGCTGCCCAAGTCTCCTAAAATCCATCCTCTACAAAGCAGAATTCTAGATCAGAAGGAGGACGGTTG
  TGTTGTGGATATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTTGCCTATGCCACTGTGAATGGCTCT
  CTGGTTGGCTGGGACCTTAGGTCTTCAAGCAATGCGTGGACTTTAAAGCATGATTTAAAGTCGGGCCTCA
  TCACTTCCTTTGCTGTGGACATCCACCAATGCTGGCTCTGCATTGGTACAAGCAGTGGTACCATGGCTTG
  TTGGGACATGAGGTTCCAGTTGCCAATTTCAAGTCACTGTCATCCTTCCAGGGCTCGAATCAGACGCCTC
  TCAATGCACCCTCTGTATCAGTCCTGGGTGATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATGTGGG
  ACATGGAGACTGGTGACAGAAGATTTACTCTCTGGGCCAGCAGTGCACCACCACTTTCTGAATTACAGCC
  TTCTCCTCATAGCGTCCATGGTATCTACTGTAGTCCTGCAGATGGAAATCCTATCCTACTAACAGCTGGC
  TCAGATATGAAAATAAGGTTTTGGGACTTGGCTTACCCAGAAAGGTCCTATGTTGTTGCAGGAAGTACTA
  GTTCCCCATCTGTGTCCTACTACAGGAAAATAATTGAAGGCACTGAAGTTGTCCAGGAAATTCAGAATAA
  GCAGAAAGTAGGACCAAGTGATGACACCCCTCGAAGGGGCCCAGAGTCCCTGCCCGTGGGACATCATGAC
  ATCATCACTGATGTCGCCACATTCCAGACCACACAGGGCTTCATCGTAACTGCTTCTAGAGATGGGATTG
  TGAAGGTGTGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210308 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQE
  LEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAH
  KSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFAT
  ELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNK
  IEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDL
  GNIIHNLCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITP
  YLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDINIYPEYILPGIAHLAQDDATIVRLAYAENIALLA
  ETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTLMENG
  ITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDAEEF
  VIVKALYALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLD
  PYITQPIIQIERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNGSLPDCPPPEDPAIAQL
  LKKLLSQGMTEEEEDKLLALKDFMMKSNKAKANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEP
  DDKRARKHVKQDSNVNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICVPLSTSSQVPE
  VTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWESKPPPPGWR
  PKGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTF
  CQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGS
  LVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQCWLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRL
  SMHPLYQSWVIAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAG
  SDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHD
  IITDVATFQTTQGFIVTASRDGIVKVWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014602
• ORF Size: 4074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014602.1, NM_014602.2, NP_055417.1
• RefSeq Size: 5078 bp
• RefSeq ORF: 4077 bp
• Locus ID: 30849
• Domains: pkinase, WD40, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Regulation of autophagy
• MW: 153.1 kDa
• Gene Summary: Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). [UniProtKB/Swiss-Prot Function]