Orexin Receptor 1 (HCRTR1) (NM_001525) Human Tagged ORF Clone

CAT#: RC210381

HCRTR1 (Myc-DDK-tagged)-Human hypocretin (orexin) receptor 1 (HCRTR1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HCRTR1
• Synonyms: OX1R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210381 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCTCAGCCACCCCAGGGGCCCAGATGGGGGTCCCCCCTGGCAGCAGAGAGCCGTCCCCTGTGC
  CTCCAGACTATGAAGATGAGTTTCTCCGCTATCTGTGGCGTGATTATCTGTACCCAAAACAGTATGAGTG
  GGTCCTCATCGCAGCCTATGTGGCTGTGTTCGTCGTGGCCCTGGTGGGCAACACGCTGGTCTGCCTGGCC
  GTGTGGCGGAACCACCACATGAGGACAGTCACCAACTACTTCATTGTCAACCTGTCCCTGGCTGACGTTC
  TGGTGACTGCTATCTGCCTGCCGGCCAGCCTGCTGGTGGACATCACTGAGTCCTGGCTGTTCGGCCATGC
  CCTCTGCAAGGTCATCCCCTATCTACAGGCTGTGTCTGTGTCAGTGGCAGTGCTAACTCTCAGCTTCATC
  GCCCTGGACCGCTGGTATGCCATCTGCCACCCACTATTGTTCAAGAGCACAGCCCGGCGGGCCCGTGGCT
  CCATCCTGGGCATCTGGGCTGTGTCGCTGGCCATCATGGTGCCCCAGGCTGCAGTCATGGAATGCAGCAG
  TGTGCTGCCTGAGCTAGCCAACCGCACACGGCTCTTCTCAGTCTGTGATGAACGCTGGGCAGATGACCTC
  TATCCCAAGATCTACCACAGTTGCTTCTTTATTGTCACCTACCTGGCCCCACTGGGCCTCATGGCCATGG
  CCTATTTCCAGATATTCCGCAAGCTCTGGGGCCGCCAGATCCCCGGCACCACCTCAGCACTGGTGCGGAA
  CTGGAAGCGCCCCTCAGACCAGCTGGGGGACCTGGAGCAGGGCCTGAGTGGAGAGCCCCAGCCCCGGGCC
  CGCGCCTTCCTGGCTGAAGTGAAGCAGATGCGTGCACGGAGGAAGACAGCCAAGATGCTGATGGTGGTGC
  TGCTGGTCTTCGCCCTCTGCTACCTGCCCATCAGCGTCCTCAATGTCCTTAAGAGGGTGTTCGGGATGTT
  CCGCCAAGCCAGTGACCGCGAAGCTGTCTACGCCTGCTTCACCTTCTCCCACTGGCTGGTGTACGCCAAC
  AGCGCTGCCAACCCCATCATCTACAACTTCCTCAGTGGCAAATTCCGGGAGCAGTTTAAGGCTGCCTTCT
  CCTGCTGCCTGCCTGGCCTGGGTCCCTGCGGCTCTCTGAAGGCCCCTAGTCCCCGCTCCTCTGCCAGCCA
  CAAGTCCTTGTCCTTGCAGAGCCGATGCTCCATCTCCAAAATCTCTGAGCATGTGGTGCTCACCAGCGTC
  ACCACAGTGCTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210381 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPSATPGAQMGVPPGSREPSPVPPDYEDEFLRYLWRDYLYPKQYEWVLIAAYVAVFVVALVGNTLVCLA
  VWRNHHMRTVTNYFIVNLSLADVLVTAICLPASLLVDITESWLFGHALCKVIPYLQAVSVSVAVLTLSFI
  ALDRWYAICHPLLFKSTARRARGSILGIWAVSLAIMVPQAAVMECSSVLPELANRTRLFSVCDERWADDL
  YPKIYHSCFFIVTYLAPLGLMAMAYFQIFRKLWGRQIPGTTSALVRNWKRPSDQLGDLEQGLSGEPQPRA
  RAFLAEVKQMRARRKTAKMLMVVLLVFALCYLPISVLNVLKRVFGMFRQASDREAVYACFTFSHWLVYAN
  SAANPIIYNFLSGKFREQFKAAFSCCLPGLGPCGSLKAPSPRSSASHKSLSLQSRCSISKISEHVVLTSV
  TTVLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001525
• ORF Size: 1275 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001525.1, NM_001525.2, NP_001516.1
• RefSeq Size: 2001 bp
• RefSeq ORF: 1278 bp
• Locus ID: 3061
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 47.5 kDa
• Gene Summary: The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009]