RHCE (NM_020485) Human Tagged ORF Clone

CAT#: RC210415

RHCE (Myc-DDK-tagged)-Human Rh blood group, CcEe antigens (RHCE), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402791) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210415 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RHCE
• Synonyms: CD240CE; RH; Rh4; RH30A; RHC; RHCe(152N); RHE; RhIVb(J); RHIXB; RHNA; RHPI; RhVI; RhVIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210415 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCTAAGTACCCGCGGTCTGTCCGGCGCTGCCTGCCCCTCTGCGCCCTAACACTGGAAGCAGCTC
  TCATTCTCCTCTTCTATTTTTTTACCCACTATGACGCTTCCTTAGAGGATCAAAAGGGGCTCGTGGCATC
  CTATCAAGTTGGCCAAGATCTGACCGTGATGGCGGCCATTGGCTTGGGCTTCCTCACCTCGAGTTTCCGG
  AGACACAGCTGGAGCAGTGTGGCCTTCAACCTCTTCATGCTGGCGCTTGGTGTGCAGTGGGCAATCCTGC
  TGGACGGCTTCCTGAGCCAGTTCCCTTCTGGGAAGGTGGTCATCACACTGTTCAGTATTCGGCTGGCCAC
  CATGAGTGCTATGTCGGTGCTGATCTCAGCGGGTGCTGTCTTGGGGAAGGTCAACTTGGCGCAGTTGGTG
  GTGATGGTGCTGGTGGAGGTGACAGCTTTAGGCACCCTGAGGATGGTCATCAGTAATATCTTCAACACAG
  ACTACCACATGAACCTGAGGCACTTCTACGTGTTCGCAGCCTATTTTGGGCTGACTGTGGCCTGGTGCCT
  GCCAAAGCCTCTACCCAAGGGAACGGAGGATAATGATCAGAGAGCAACGATACCCAGTTTGTCTGCCATG
  CTGGGCGCCCTCTTCTTGTGGATGTTCTGGCCAAGTGTCAACTCTGCTCTGCTGAGAAGTCCAATCCAAA
  GGAAGAATGCCATGTTCAACACCTACTATGCTCTAGCAGTCAGTGTGGTGACAGCCATCTCAGGGTCATC
  CTTGGCTCACCCCCAAAGGAAGATCAGCATGACTTATGTGCACAGTGCGGTGTTGGCAGGAGGCGTGGCT
  GTGGGTACCTCGTGTCACCTGATCCCTTCTCCGTGGCTTGCCATGGTGCTGGGTCTTGTGGCTGGGCTGA
  TCTCCATCGGGGGAGCCAAGTGCCTGCCGGTGTGTTGTAACCGAGTGCTGGGGATTCACCACATCTCCGT
  CATGCACTCCATCTTCAGCTTGCTGGGTCTGCTTGGAGAGATCACCTACATTGTGCTGCTGGTGCTTCAT
  ACTGTCTGGAACGGCAATGGCATGATTGGCTTCCAGGTCCTCCTCAGCATTGGGGAACTCAGCTTGGCCA
  TCGTGATAGCTCTCACGTCTGGTCTCCTGACAGGTTTGCTCCTAAATCTCAAAATATGGAAAGCACCTCA
  TGTGGCTAAATATTTTGATGACCAAGTTTTCTGGAAGTTTCCTCATTTGGCTGTTGGATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210415 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSKYPRSVRRCLPLCALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAAIGLGFLTSSFR
  RHSWSSVAFNLFMLALGVQWAILLDGFLSQFPSGKVVITLFSIRLATMSAMSVLISAGAVLGKVNLAQLV
  VMVLVEVTALGTLRMVISNIFNTDYHMNLRHFYVFAAYFGLTVAWCLPKPLPKGTEDNDQRATIPSLSAM
  LGALFLWMFWPSVNSALLRSPIQRKNAMFNTYYALAVSVVTAISGSSLAHPQRKISMTYVHSAVLAGGVA
  VGTSCHLIPSPWLAMVLGLVAGLISIGGAKCLPVCCNRVLGIHHISVMHSIFSLLGLLGEITYIVLLVLH
  TVWNGNGMIGFQVLLSIGELSLAIVIALTSGLLTGLLLNLKIWKAPHVAKYFDDQVFWKFPHLAVGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020485
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020485.1, NM_020485.2, NM_020485.3, NM_020485.4, NP_065231.2, NP_065231.3
• RefSeq Size: 1618 bp
• RefSeq ORF: 1254 bp
• Locus ID: 6006
• Cytogenetics: 1p36.11
• Protein Families: Transmembrane
• MW: 45.4 kDa
• Gene Summary: 'The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Aug 2016]'