RHCE (NM_020485) Human Tagged ORF Clone
CAT#: RC210415
RHCE (Myc-DDK-tagged)-Human Rh blood group, CcEe antigens (RHCE), transcript variant 1
"NM_020485" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RHCE |
Synonyms | CD240CE; RH; Rh4; RH30A; RHC; RHCe(152N); RHE; RhIVb(J); RHIXB; RHNA; RHPI; RhVI; RhVIII |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210415 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCTAAGTACCCGCGGTCTGTCCGGCGCTGCCTGCCCCTCTGCGCCCTAACACTGGAAGCAGCTC TCATTCTCCTCTTCTATTTTTTTACCCACTATGACGCTTCCTTAGAGGATCAAAAGGGGCTCGTGGCATC CTATCAAGTTGGCCAAGATCTGACCGTGATGGCGGCCATTGGCTTGGGCTTCCTCACCTCGAGTTTCCGG AGACACAGCTGGAGCAGTGTGGCCTTCAACCTCTTCATGCTGGCGCTTGGTGTGCAGTGGGCAATCCTGC TGGACGGCTTCCTGAGCCAGTTCCCTTCTGGGAAGGTGGTCATCACACTGTTCAGTATTCGGCTGGCCAC CATGAGTGCTATGTCGGTGCTGATCTCAGCGGGTGCTGTCTTGGGGAAGGTCAACTTGGCGCAGTTGGTG GTGATGGTGCTGGTGGAGGTGACAGCTTTAGGCACCCTGAGGATGGTCATCAGTAATATCTTCAACACAG ACTACCACATGAACCTGAGGCACTTCTACGTGTTCGCAGCCTATTTTGGGCTGACTGTGGCCTGGTGCCT GCCAAAGCCTCTACCCAAGGGAACGGAGGATAATGATCAGAGAGCAACGATACCCAGTTTGTCTGCCATG CTGGGCGCCCTCTTCTTGTGGATGTTCTGGCCAAGTGTCAACTCTGCTCTGCTGAGAAGTCCAATCCAAA GGAAGAATGCCATGTTCAACACCTACTATGCTCTAGCAGTCAGTGTGGTGACAGCCATCTCAGGGTCATC CTTGGCTCACCCCCAAAGGAAGATCAGCATGACTTATGTGCACAGTGCGGTGTTGGCAGGAGGCGTGGCT GTGGGTACCTCGTGTCACCTGATCCCTTCTCCGTGGCTTGCCATGGTGCTGGGTCTTGTGGCTGGGCTGA TCTCCATCGGGGGAGCCAAGTGCCTGCCGGTGTGTTGTAACCGAGTGCTGGGGATTCACCACATCTCCGT CATGCACTCCATCTTCAGCTTGCTGGGTCTGCTTGGAGAGATCACCTACATTGTGCTGCTGGTGCTTCAT ACTGTCTGGAACGGCAATGGCATGATTGGCTTCCAGGTCCTCCTCAGCATTGGGGAACTCAGCTTGGCCA TCGTGATAGCTCTCACGTCTGGTCTCCTGACAGGTTTGCTCCTAAATCTCAAAATATGGAAAGCACCTCA TGTGGCTAAATATTTTGATGACCAAGTTTTCTGGAAGTTTCCTCATTTGGCTGTTGGATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210415 protein sequence
Red=Cloning site Green=Tags(s) MSSKYPRSVRRCLPLCALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAAIGLGFLTSSFR RHSWSSVAFNLFMLALGVQWAILLDGFLSQFPSGKVVITLFSIRLATMSAMSVLISAGAVLGKVNLAQLV VMVLVEVTALGTLRMVISNIFNTDYHMNLRHFYVFAAYFGLTVAWCLPKPLPKGTEDNDQRATIPSLSAM LGALFLWMFWPSVNSALLRSPIQRKNAMFNTYYALAVSVVTAISGSSLAHPQRKISMTYVHSAVLAGGVA VGTSCHLIPSPWLAMVLGLVAGLISIGGAKCLPVCCNRVLGIHHISVMHSIFSLLGLLGEITYIVLLVLH TVWNGNGMIGFQVLLSIGELSLAIVIALTSGLLTGLLLNLKIWKAPHVAKYFDDQVFWKFPHLAVGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020485 |
ORF Size | 1251 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020485.1, NM_020485.2, NM_020485.3, NM_020485.4, NP_065231.2, NP_065231.3 |
RefSeq Size | 1618 bp |
RefSeq ORF | 1254 bp |
Locus ID | 6006 |
Cytogenetics | 1p36.11 |
Protein Families | Transmembrane |
MW | 45.4 kDa |
Gene Summary | 'The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Aug 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC308833 | RHCE (untagged)-Human Rh blood group, CcEe antigens (RHCE), transcript variant 1 |
USD 760.00 |
|
RG210415 | RHCE (GFP-tagged) - Human Rh blood group, CcEe antigens (RHCE), transcript variant 1 |
USD 460.00 |
|
RC210415L1 | Lenti ORF clone of Human Rh blood group, CcEe antigens (RHCE), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC210415L2 | Lenti ORF clone of Human Rh blood group, CcEe antigens (RHCE), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC210415L3 | Lenti ORF clone of Human Rh blood group, CcEe antigens (RHCE), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC210415L4 | Lenti ORF clone of Human Rh blood group, CcEe antigens (RHCE), transcript variant 1, mGFP tagged |
USD 620.00 |
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