TFII I (GTF2I) (NM_033001) Human Tagged ORF Clone

CAT#: RC210502L4

Lenti-ORF clone of GTF2I (mGFP-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: GTF2I
• Synonyms: BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC210502).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_033001
• ORF Size: 2931 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033001.2, NP_127494.1
• RefSeq Size: 4466 bp
• RefSeq ORF: 2934 bp
• Locus ID: 2969
• Cytogenetics: 7q11.23
• Domains: GTF2I
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 110.1 kDa
• Gene Summary: 'This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]'