MIDN (NM_177401) Human Tagged ORF Clone

CAT#: RC210528

MIDN (Myc-DDK-tagged)-Human midnolin (MIDN)

Product Images

Western blot validation of overexpression lysate (Cat# LY406151) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210528 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MIDN
• Synonyms: DKFZp547M072
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210528 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCAGCCCGGCGGCGCCCGGAGCTGCCGGCGCGGGGCCCCCGGCGGCGCCTGCGAGCTGGGCC
  CGGCGGCCGAGGCGGCGCCCATGAGCCTCGCCATCCACAGCACCACGGGCACCCGCTACGACCTGGCCGT
  GCCGCCCGACGAGACGGTGGAGGGGCTGCGCAAGCGGTTGTCCCAGCGCCTCAAAGTGCCCAAGGAGCGC
  CTGGCTCTTCTCCACAAAGACACCCGGCTCAGTTCGGGGAAGCTGCAGGAGTTCGGCGTGGGTGATGGCA
  GCAAGCTGACCTTGGTACCCACCGTGGAAGCGGGCCTCATGTCTCAGGCCTCAAGGCCGGAACAGTCCGT
  GATGCAAGCTCTCGAGAGTCTCACGGAGACGCAGGTCAGTGACTTCCTGTCGGGCCGTTCGCCACTGACA
  CTGGCCTTGCGTGTGGGCGACCACATGATGTTCGTGCAGCTGCAGCTCGCGGCCCAGCACGCTCCACTGC
  AACACCGCCATGTGCTGGCCGCTGCGGCCGCCGCCGCTGCTGCGCGGGGGGACCCCAGCATAGCCTCCCC
  CGTGTCCTCGCCCTGCCGGCCGGTGTCCAGTGCCGCCCGAGTCCCCCCGGTGCCCACCAGCCCGTCCCCT
  GCATCTCCCTCGCCCATCACAGCCGGCTCCTTCCGGTCCCACGCAGCCTCCACCACCTGCCCGGAGCAGA
  TGGACTGCTCCCCCACGGCCAGCAGCAGTGCCAGTCCTGGTGCCAGCACCACGTCTACCCCAGGGGCCAG
  CCCTGCCCCCCGCTCCCGAAAACCCGGCGCCGTCATCGAGAGCTTTGTGAATCACGCCCCGGGGGTCTTC
  TCAGGGACCTTCTCTGGCACGCTACACCCCAACTGCCAAGACAGCAGCGGGCGGCCGCGGCGTGACATCG
  GCACCATCCTGCAGATCCTGAACGACCTCCTGAGCGCCACCCGGCACTACCAGGGCATGCCCCCTTCGCT
  GGCCCAGCTCCGCTGCCACGCCCAGTGCTCCCCGGCCTCACCGGCCCCCGACCTGGCCCCCAGAACTACC
  TCCTGCGAGAAGCTCACGGCTGCCCCCTCAGCCTCCCTGCTGCAGGGCCAGAGCCAGATCCGCATGTGCA
  AGCCCCCGGGGGATCGGCTTCGGCAGACAGAAAACCGCGCCACGCGCTGCAAGGTGGAACGGCTGCAGCT
  GCTTCTGCAGCAGAAACGGCTCCGTAGAAAGGCCCGGCGGGACGCGCGGGGTCCGTACCACTGGTCACCC
  AGCCGCAAGGCCGGCCGCAGCGACAGCAGTAGCAGCGGGGGCGGCGGCAGCCCCAGCGAGGCCTCCGGCT
  TGGGCCTCGACTTCGAGGACTCCGTGTGGAAGCCAGAAGCCAACCCTGACATCAAGTCAGAGTTCGTGGT
  GGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210528 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPQPGGARSCRRGAPGGACELGPAAEAAPMSLAIHSTTGTRYDLAVPPDETVEGLRKRLSQRLKVPKER
  LALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQVSDFLSGRSPLT
  LALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAAAAARGDPSIASPVSSPCRPVSSAARVPPVPTSPSP
  ASPSPITAGSFRSHAASTTCPEQMDCSPTASSSASPGASTTSTPGASPAPRSRKPGAVIESFVNHAPGVF
  SGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLAQLRCHAQCSPASPAPDLAPRTT
  SCEKLTAAPSASLLQGQSQIRMCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWSP
  SRKAGRSDSSSSGGGGSPSEASGLGLDFEDSVWKPEANPDIKSEFVVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177401
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177401.4, NP_796375.3
• RefSeq Size: 3812
• RefSeq ORF: 1407
• Locus ID: 90007
• Protein Families: Druggable Genome
• MW: 49.2 kDa