GMPR2 (NM_001002001) Human Tagged ORF Clone

CAT#: RC210599

GMPR2 (Myc-DDK-tagged)-Human guanosine monophosphate reductase 2 (GMPR2), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY424315) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210599 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GMPR2 protein (Cat# TP310599). The protein was produced from HEK293T cells transfected with GMPR2 cDNA clone (Cat# RC210599) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GMPR2
• Synonyms: GMPR 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210599 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCATATTGACAACGATGTGAAACTGGACTTCAAGGATGTCCTTTTGAGGCCCAAACGCAGTACCC
  TTAAGTCTCGAAGTGAGGTGGATCTCACAAGATCCTTTTCATTTCGGAACTCAAAGCAGACATACTCTGG
  GGTTCCCATCATTGCTGCCAATATGGATACTGTGGGCACCTTTGAGATGGCCAAGGTTCTCTGTAAGTTC
  TCTCTCTTCACTGCTGTCCATAAGCACTATAGCCTCGTTCAGTGGCAAGAGTTTGCTGGCCAGAATCCTG
  ACTGTCTTGAGCATCTGGCTGCCAGCTCAGGCACAGGCTCTTCTGACTTTGAGCAGCTGGAACAGATCCT
  GGAAGCTATTCCCCAGGTGAAGTATATATGCCTGGATGTGGCAAATGGCTACTCTGAACACTTTGTTGAA
  TTTGTAAAAGATGTACGGAAGCGCTTCCCCCAGCACACCATCATGGCAGGGAATGTGGTAACAGGAGAGA
  TGGTAGAAGAGCTCATCCTTTCTGGGGCTGACATCATCAAAGTGGGAATTGGGCCAGGCTCTGTGTGTAC
  TACTCGGAAGAAAACTGGAGTGGGGTATCCACAGCTCAGCGCAGTGATGGAGTGTGCAGATGCTGCTCAT
  GGCCTCAAAGGCCACATCATTTCAGATGGAGGTTGCAGCTGTCCTGGGGATGTGGCCAAGGCTTTTGGGG
  CAGGAGCTGACTTCGTGATGCTGGGTGGCATGCTGGCTGGGCACAGTGAGTCAGGTGGTGAGCTCATCGA
  GAGGGATGGCAAGAAGTACAAGCTCTTCTATGGAATGAGTTCTGAAATGGCCATGAAGAAGTATGCTGGG
  GGCGTGGCTGAGTACAGAGCCTCAGAGGGAAAGACAGTGGAAGTTCCTTTTAAAGGAGATGTGGAACATA
  CCATCCGAGACATCCTAGGAGGGATCCGCTCTACGTGTACCTATGTGGGAGCAGCTAAGCTCAAAGAGTT
  GAGCAGGAGAACTACCTTCATCCGAGTCACCCAGCAGGTGAATCCAATCTTCAGTGAGGCGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210599 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPHIDNDVKLDFKDVLLRPKRSTLKSRSEVDLTRSFSFRNSKQTYSGVPIIAANMDTVGTFEMAKVLCKF
  SLFTAVHKHYSLVQWQEFAGQNPDCLEHLAASSGTGSSDFEQLEQILEAIPQVKYICLDVANGYSEHFVE
  FVKDVRKRFPQHTIMAGNVVTGEMVEELILSGADIIKVGIGPGSVCTTRKKTGVGYPQLSAVMECADAAH
  GLKGHIISDGGCSCPGDVAKAFGAGADFVMLGGMLAGHSESGGELIERDGKKYKLFYGMSSEMAMKKYAG
  GVAEYRASEGKTVEVPFKGDVEHTIRDILGGIRSTCTYVGAAKLKELSRRTTFIRVTQQVNPIFSEAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002001
• ORF Size: 1044 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002001.1, NM_001002001.2, NP_001002001.1
• RefSeq Size: 1930 bp
• RefSeq ORF: 1047 bp
• Locus ID: 51292
• Protein Families: Druggable Genome
• Protein Pathways: Purine metabolism
• MW: 37.9 kDa
• Gene Summary: This gene encodes an enzyme that catalyzes the irreversible and NADPH-dependent reductive deamination of guanosine monophosphate (GMP) to inosine monophosphate (IMP). The protein also functions in the re-utilization of free intracellular bases and purine nucleosides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2017]